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Overexpression of PRAT1 protein is closely related to triple-negative breast cancer
Sang Eun Nam,Young-Sin Ko,Kyoung Sik Park,TongYi Jin,Young-Bum Yoo,Jung-Hyun Yang,Wook-Youn Kim,Hye-Seung Han,So-Dug Lim,Seung Eun Lee,Wan-Seop Kim 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.103 No.2
Purpose: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a poor prognosis and a lack of targeted therapy. Overexpression of PRAT1 is thought to be associated with this aggressive subtype of cancer. Here, we performed a comprehensive analysis and assessed the association between overexpression of PRAT1 and TNBC. Methods: First, using different web-based bioinformatics platforms (TIMER 2.0, UALCAN, and GEPIA 2), the expression of was assessed. Then, the expression of the PRAT1 protein and hormone receptors and HER2 status were assessed by immunohistochemical analysis. For samples of tumors with equivocal immunoreactivity, we performed silver in situ hybridization of the HER2 gene to determine an accurate HER2 status. Next, we used the R package and bc-GenExMiner 4.8 to analyze the relationship between PRAT1 expression and clinicopathological parameters in breast cancer patients. Finally, we determined the relationship between PRAT1 overexpression and prognosis in patients. Results: The expression of PRAT1 in breast cancer tissues is significantly higher than in normal tissue. PRAT1 expression was significantly related to worse overall survival (P < 0.05) and was correlated with these clinicopathological features: T stage, N stage, age, high histologic grade, estrogen receptor status, progesterone receptor status, Her-2 status, TNBC status, basal-like status, CK5/6 status, and Ki67 status. Conclusion: PRAT1 was overexpressed in breast cancer compared to normal tissue, and it may be involved in the progression of breast cancer malignancy. This study provides suggestive evidence of the prognostic role of PRAT1 in breast cancer and the therapeutic target for TNBC.
Improvement Strategy According to the Change of Hotel Environment
Lim, Heon-Wook,Seo, Dae-Sung The International Promotion Agency of Culture Tech 2021 International Journal of Advanced Culture Technolo Vol.9 No.2
This study is to develop a strategy to prepare an improvement strategy according to the environmental change of the hotel. Currently, domestic hotels are implementing marketing through food and beverage as a countermeasure against the sales decrease, and in order to develop effective marketing plan, 5 Force Model environmental analysis and STP analysis are analyzed. 5 Force Model Environmental Analysis showed that domestic hotels are facing various difficulties such as the expansion of accommodation sharing system, the decrease of Chinese tourists due to the THAAD problem, the increase of hotels, the introduction of PMS, the increase of minimum wage, the introduction of 52 hours work week, and the increase in product preference As an STP response strategy to correspond these difficulties, it is necessary to develop products for the main customers of the hotel food and beverage, such as those in the 20s-30s, the workers, smartphones and SNS users. And also hotels should seek ways to lower price of the product to the level desired by the user to compete against substitutes. In conclusion we suggest that hotels are committed to fulfilling their role by meeting guest safety and COVID-19 compliance requirements, but a focus on immediate cleanliness and quarantine against infectious diseases, like Airbnb, will enable greater growth.
Arabidopsis PYL8 Plays an Important Role for ABA Signaling and Drought Stress Responses
Lim, Chae Woo,Baek, Woonhee,Han, Sang-Wook,Lee, Sung Chul The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.4
Plants are frequently exposed to numerous environmental stresses such as dehydration and high salinity, and have developed elaborate mechanisms to counteract the deleterious effects of stress. The phytohormone abscisic acid (ABA) plays a critical role as an integrator of plant responses to water-limited condition to activate ABA signal transduction pathway. Although perception of ABA has been suggested to be important, the function of each ABA receptor remains elusive in dehydration condition. Here, we show that ABA receptor, pyrabactin resistance-like protein 8 (PYL8), functions in dehydration conditions. Transgenic plants overexpressing PYL8 exhibited hypersensitive phenotype to ABA in seed germination, seedling growth and establishment. We found that hypersensitivity to ABA of transgenic plants results in high degrees of stomatal closure in response to ABA leading to low transpiration rates and ultimately more vulnerable to drought than the wild-type plants. In addition, high expression of ABA maker genes also contributes to altered drought tolerance phenotype. Overall, this work emphasizes the importance of ABA signaling by ABA receptor in stomata during defense response to drought stress.
Lim, Mi-Sun,Chang, Mi-Yoon,Kim, Sang-Mi,Yi, Sang-Hoon,Suh-Kim, Haeyoung,Jung, Sung Jun,Kim, Min Jung,Kim, Jin Hyuk,Lee, Yong-Sung,Lee, Soo Young,Kim, Dong-Wook,Lee, Sang-Hun,Park, Chang-Hwan American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.28
<P>Recent groundbreaking work has demonstrated that combined expression of the transcription factors <I>Brn2</I>, <I>Ascl1</I>, and <I>Myt1L</I> (BAM; also known as Wernig factors) convert mouse fibroblasts into postmitotic neuronal cells. However, questions remain regarding whether trans-conversion is achieved directly or involves an intermediary precursor stage. Trans-conversion toward expandable neural precursor cells (NPCs) is more useful than direct one-step neuron formation with respect to yielding a sufficient number of cells and the feasibility of manipulating NPC differentiation toward certain neuron subtypes. Here, we show that co-expression of Wernig factors and <I>Bcl-xL</I> induces fibroblast conversion into NPCs (induced NPCs (iNPCs)) that are highly expandable for >100 passages. Gene expression analyses showed that the iNPCs exhibited high expression of common NPC genes but not genes specific to defined embryonic brain regions. This finding indicated that a regional identity of iNPCs was not established. Upon induction, iNPCs predominantly differentiated into astrocytes. However, the differentiation potential was not fixed and could be efficiently manipulated into general or specific subtypes of neurons by expression of additional genes. Specifically, overexpression of <I>Nurr1</I> and <I>Foxa2</I>, transcription factors specific for midbrain dopamine neuron development, drove iNPCs to yield mature midbrain dopamine neurons equipped with presynaptic DA neuronal functions. We further assessed the therapeutic potential of iNPCs in Parkinson disease model rats.</P>
Three-dimensional hierarchical Te-Si nanostructures.
Lim, Jae-Hong,Shin, Gyeong-Jin,Hwang, Tae-Yeon,Lim, Hyo-Ryoung,Lee, Young-In,Lee, Kyu-Hwan,Kim, Sung-Dae,Oh, Min-Wook,Park, Su-Dong,Myung, Nosang V,Choa, Yong-Ho RSC Pub 2014 Nanoscale Vol.6 No.20
<P>Three-dimensional hybrid nanostructures (i.e., Te 'nanobranches' on a Si 'nanotrunk' or Te 'nanoleaves' on a Si 'nanotrunk') were synthesized by combining the gold-assisted chemical etching of Si to form Si 'nanotrunks' and the galvanic displacement of Si to form Te 'nanobranches' or 'nanoleaves.' By adjusting the composition of the electrolyte used for the galvanic displacement reaction, the shape of the Te nanostructures could be changed from nanoleaves to nanobranches. The Si nanotrunks with Te nanobranches showed stronger luminescent emission in the visible region, with their Raman spectrum having a higher wave number, owing to their grain size being larger. This suggested that the optical and photoelectrochemical properties of Te-Si hybrid nanostructures depend on their shape and size. Using this approach, it should be possible to fabricate various hierarchical nanostructures for use in photoelectronic and photoelectrochemical devices.</P>
Lim, Chae Woo,Baek, Woonhee,Lim, Sohee,Han, Sang-Wook,Lee, Sung Chul APS Press 2015 Molecular plant-microbe interactions Vol.28 No.7
<P>A pepper bZIP transcription factor gene, CabZIP2, was isolated from pepper leaves infected with a virulent strain of Xanthomonas campestris pv. vesicatoria. Transient expression analysis of the CabZIP2-GFP fusion protein in Nicotiana benthamiana revealed that the CabZIP2 protein is localized in the cytoplasm as well as the nucleus. The acidic domain in the N-terminal region of CabZIP2 that is fused to the GAL4 DNA-binding domain is required to activate the transcription of reporter genes in yeast. Transcription of CabZIP2 is induced in pepper plants inoculated with virulent or avirulent strains of X. campestris pv. vesicatoria. The CabZIP2 gene is also induced by defense-related hormones such as salicylic acid, methyl jasmonate, and ethylene. To elucidate the in vivo function of the CabZIP2 gene in plant defense, virus-induced gene silencing in pepper and overexpression in Arabidopsis were used. CabZIP2-silenced pepper plants were susceptible to infection by the virulent strain of X. campestris pv. vesicatoria, which was accompanied by reduced expression of defense-related genes such as CaBPR1 and CaAMP1. CabZIP2 overexpression in transgenic Arabidopsis plants conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3000. Together, these results suggest that CabZIP2 is involved in bacterial disease resistance.</P>
A 5.8-Gb/s Adaptive Integrating Duobinary DFE Receiver for Multi-Drop Memory Interface
Lim, Hyun-Wook,Choi, Sung-Won,Ahn, Jeong-Keun,Min, Woong-Ki,Lee, Sang-Kyu,Baek, Chang-Hoon,Lee, Jae-Youl,Hwang, Gyoo-Cheol,Jun, Young-Hyun,Kong, Bai-Sun Institute of Electrical and Electronics Engineers 2017 IEEE journal of solid-state circuits Vol. No.
<P>This paper describes a 5.8 Gb/s adaptive integrating duobinary decision-feedback equalizer (DFE) for use in next-generation multi-drop memory interface. The proposed receiver combines traditional interface techniques like the integrated signaling and the duobinary signaling, in which the duobinary signal is generated by current integration in the receiver. It can address issues such as input data dependence during integration, need for precursor equalization, high equalizer gain boosting, and sensitivity to high-frequency noise. The proposed receiver also alleviates DFE critical timing to provide gain in speed, and embed DFE taps in duobinary decoding to provide gain in power and area. The adaptation for adjusting the equalizer common-mode level, duobinary zero level, tap coefficient values, and timing recovery is incorporated. The proposed DFE receiver was fabricated in a 45 nm CMOS process, whose measurement results indicated that it worked at 5.8 Gb/s speed in a four-drop channel configuration with seven slave ICs, and the bathtub curve shows 36% open for 10(-10) bit error rate.</P>