http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Eun-Jung,Oh, Se-Wook,Lee, Nam-Hyouck,Kim, Young-Ho,Lee, Dong-Un,Yamamoto, Katsuhiro,Kim, Yun-Ji Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.3
In order to reduce the increased hardness of beef bulgogi due to hydrostatic pressure (HP), kiwifruit (Actinidia chinensis) was applied. To understand the changes of shear force in beef bulgogi with kiwifruit induced by HP, changes in chemical properties of myofibril (Mf) with 10% kiwifruit induced by HP were investigated. From the SDS-PAGE patterns of Mf with 10% kiwifruit, there was an observed increase in the degradation of myosin heavy chain (MHC) by HP (300-500 MPa) to that by 0.1 MPa. This result indicates that HP may enhance enzyme action from a kiwifruit for the degradation of MHC, and the similar phenomenon occurred in the beef bulgogi with kiwifruit induced by HP. The shear force of beef bulgogi without a kiwifruit induced by 400 and 500 MPa significantly increased compared to that by 0.1 MPa (p<0.05). However, in the beef bulgogi with 10% or 20% kiwifruit, the shear force induced by 400 or 500 MPa was similar or slightly lower than that by 0.1 MPa. Consequently, adding kiwifruit to bulgogi could reduce the hardness of HP-induced beef bulgogi due to the enzyme action in the kiwifruit accelerated by HP.
Inhibition of Transglutaminase and Microbial Transglutaminase Activity by Garlic
Lee, Nam-Hyouck,Takeuchi, Atsuyoshi,Konno, Kunihiko Korean Society of Food Science and Technology 2007 Food Science and Biotechnology Vol.16 No.2
Ground garlic inhibited the cross-linking reaction of myosin and incorporation of monodansylcadaverine (MDC) in salted Alaska pollack surimi catalyzed by transglutaminase (TGase). The component responsible for the inhibition was a thermostable, low molecular weight compound. The component also inhibited microbial transglutaminase (MTGase). The inhibition by garlic was reversibly recovered upon addition of 2-mercaptoethanol. The inhibitory component was therefore hypothesized to contain sulfhydryl groups within its structure. Alliin itself did not inhibit the cross-linking reaction. However, the addition of alliin together with garlic increased the inhibition. This result suggested that compounds derived from alliin was responsible for the inhibition of TGase activity.
장류에 접종된 Bacillus cereus 포자의 저감화 기법에 관한 연구
이남혁(Nam-Hyouck Lee),조은지(Eun-Ji Jo),오세욱(Se-Wook Oh),홍상필(Sang-Pil Hong) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.12
된장과 고추장에 대하여 Bacillus cereus 포자를 105 spore/g 접종하고 이의 저감화에 적용 가능한 허들로서 additive(95% ethanol 3%, 1% oregano 추출물 0.3%), Joule 가열(95℃, 5분) 및 초고압처리(500 MPa, 45℃, 5분)를 적용하여 Bacillus cereus 포자의 살균효과와 저장성을 분석하였다. 된장에 있어 AJ(additive+Joule 가열) 및 AJP(additive+Joule 가열+초고압) 처리구는 각각 2.80 log 및 3.74 log 감소효과를 보였고 고추장의 경우에는 각각 4.71 log 및 5.60 log 감소를 보여 Joule 가열처리구는 된장과 고추장에 있어서 additive 처리구와 함께 Bacillus cereus 포자의 살균에 상당한 시너지 효과를 나타내는 것으로 판단되었다. 한편 30℃ 저장시험에서 additive, Joule 가열 및 초고압 병행 처리는 된장 및 고추장에서 낮은 수준의 포자를 유지하는 데에도 유효한 효과를 보여 AJ(additive+Joule 가열) 및 AJP(additive+Joule 가열+초고압) 처리기법은 된장 및 고추장의 Bacillus cereus 포자에 대한 효율적인 살균방법으로서 적용이 가능할 것으로 보였다. The effects of hurdle techniques on the reduction of Bacillus cereus spores in Doenjang and Gochujang were investigated. In our system, Bacillus cereus spores were artificially inoculated into Doenjang and Gochujang. Hurdle techniques used in this study were additives (3% ethyl alcohol-0.03% oregano extract), Joule heating (95℃ for 5 min), and hydrostatic pressure (500 MPa for 5 min at 45℃). Additive-Joule (AJ) and additive-Joulepressure (AJP) treatments for Doenjang resulted in a 2.80 log and 3.74 log reduction, respectively, while treatments for Gochujang resulted in a 4.71 log and 5.60 log reduction, respectively. This suggests a high synergistic effect of Joule heating with additive treatment in Doenjang and Gochujang. A combination of hurdles such as additives, Joule heating, and hydrostatic pressure also kept Bacillus cereus spore counts low during storage at 30℃. Therefore, Bacillus cereus spores inoculated into Doenjang and Gochujang can be effectively reduced through combined treatments, including AJ or AJP.
Jung, Sung Keun,Lee, Mee-Hyun,Lim, Do Young,Kim, Jong Eun,Singh, Puja,Lee, Sung-Young,Jeong, Chul-Ho,Lim, Tae-Gyu,Chen, Hanyong,Chi, Young-In,Kundu, Joydeb Kumar,Lee, Nam Hyouck,Lee, Charles C.,Cho, Y American Society for Biochemistry and Molecular Bi 2014 The Journal of biological chemistry Vol.289 No.52
<P>Non-small-cell lung cancer (NSCLC) is associated with diverse genetic alterations including mutation of epidermal growth factor receptor (EGFR). Isoliquiritigenin (ILQ), a chalcone derivative, possesses anticancer activities. In the present study, we investigated the effects of ILQ on the growth of tyrosine kinase inhibitor (TKI)-sensitive and -resistant NSCLC cells and elucidated its underlying mechanisms. Treatment with ILQ inhibited growth and induced apoptosis in both TKI-sensitive and -resistant NSCLC cells. ILQ-induced apoptosis was associated with the cleavage of caspase-3 and poly-(ADP-ribose)-polymerase, increased expression of Bim, and reduced expression of Bcl-2. <I>In vitro</I> kinase assay results revealed that ILQ inhibited the catalytic activity of both wild type and double mutant (L858R/T790M) EGFR. Treatment with ILQ inhibited the anchorage-independent growth of NIH3T3 cells stably transfected with either wild type or double-mutant EGFR with or without EGF stimulation. ILQ also reduced the phosphorylation of Akt and ERK1/2 in both TKI-sensitive and -resistant NSCLC cells, and attenuated the kinase activity of Akt1 and ERK2 <I>in vitro</I>. ILQ directly interacted with both wild type and double-mutant EGFR in an ATP-competitive manner. A docking model study showed that ILQ formed two hydrogen bonds (Glu-762 and Met-793) with wild type EGFR and three hydrogen bonds (Lys-745, Met-793, and Asp-855) with mutant EGFR. ILQ attenuated the xenograft tumor growth of H1975 cells, which was associated with decreased expression of Ki-67 and diminished phosphorylation of Akt and ERK1/2. Taken together, ILQ suppresses NSCLC cell growth by directly targeting wild type or mutant EGFR.</P>
Jung, Sung Keun,Ha, Su Jeong,Kim, Yeong A,Lee, Jihoon,Lim, Tae-Gyu,Kim, Yun Tai,Lee, Nam Hyouck,Park, Jun Seong,Yeom, Myeong-Hun,Lee, Hyong Joo,Lee, Ki Won Blackwell Publishing Ltd 2015 Journal of cellular and molecular medicine Vol.19 No.1
<P>Dehydroglyasperin D (DHGA-D), a compound present in licorice, has been found to exhibit anti-obesity, antioxidant and anti-aldose reductase effects. However, the direct molecular mechanism and molecular targets of DHGA-D during skin inflammation remain unknown. In the present study, we investigated the effect of DHGA-D on inflammation and its mechanism of action on UVB-induced skin inflammation in HaCaT human keratinocytes and SKH-1 hairless mice. DHGA-D treatment strongly suppressed UVB-induced COX-2 expression, PGE2 generation and AP-1 transactivity in HaCaT cells without affecting cell viability. DHGA-D also inhibited phosphorylation of the mitogen-activated protein kinase kinase (MKK) 3/6/p38, MAPK/Elk-1, MKK4/c-Jun N-terminal kinase (JNK) 1/2/c-Jun/mitogen, and stress-activated protein kinase (MSK), whereas phosphorylation of the mixed-lineage kinase (MLK) 3 remained unaffected. Kinase and co-precipitation assays with DHGA-D Sepharose 4B beads showed that DHGA-D significantly suppressed MLK3 activity through direct binding to MLK3. Knockdown of MLK3 suppressed COX-2 expression as well as phosphorylation of MKK4/p38 and MKK3/6/JNK1/2 in HaCaT cells. Furthermore, Western blot assay and immunohistochemistry results showed that DHGA-D pre-treatment significantly inhibits UVB-induced COX-2 expression <I>in vivo</I>. Taken together, these results indicate that DHGA-D may be a promising anti-inflammatory agent that mediates suppression of both COX-2 expression and the MLK3 signalling pathway through direct binding and inhibition of MLK3.</P>
Lee, Eun-Jung,Kim, Yun-Ji,Lee, Nam-Hyouck,Hong, Seok-In,Yamamoto, Katsuhiro John Wiley Sons, Ltd. 2007 Journal of the Science of Food and Agriculture Vol.87 No.1
<P>Hydrostatic pressure (HP) and heat treatments of myofibrillar proteins have both been shown to induce protein denaturation, but different gel formation properties result from these treatments. To characterise differences in the properties of proteins resulting from HP or heat treatment, Ca- and Mg-ATPase activities (ATP, adenosine triphosphate) and protein solubility in 0.1 and 0.6 mol L<SUP>−1</SUP> KCl buffers (pH 7) were evaluated in this study. The inactivation rate of Ca-ATPase of myofibrillar proteins (Mf) induced by HP was slower than that of Mg-ATPase at each of the tested pressures. However, the inactivation rate of Ca-ATPase induced by heating was faster than that of Mg-ATPase at each of the tested temperatures. The level of soluble proteins in Mf suspension induced by HP in 0.1 mol L<SUP>−1</SUP> KCl buffer increased with increasing pressure up to 400 MPa and then decreased slightly at 500 MPa. However, the level of soluble proteins in Mf suspension induced by heat treatment in 0.1 mol L<SUP>−1</SUP> KCl buffer increased with increasing temperature up to 55°C. According to the results of sodium dodecyl sulfate polyacrylamide gel electrophoresis, the levels of soluble myosin heavy chain and actin in Mf suspension induced by HP in 0.6 mol L<SUP>−1</SUP> KCl buffer decreased simultaneously at pressures higher than 300 MPa. The level of soluble MHC in 0.6 mol L<SUP>−1</SUP> KCl buffer decreased gradually with increasing temperature, but there were no changes in the level of soluble actin in 0.6 mol L<SUP>−1</SUP> KCl buffer with increasing temperature up to 50°C. These results showed that the mechanism of HP-induced protein denaturation was different from the mechanism underlying heat-induced protein denaturation. Copyright © 2006 Society of Chemical Industry</P>
Effects of Hydrostatic Pressure on Myofibrillar Protein Extracted from Bovine Semitendinosus
Lee. Eun-Jung,Kim. Yun-Ji,Lee. Nam-Hyouck,Yamamoto. Katsuhiro 한국축산식품학회 2004 심포지움 및 학술발표회 Vol.- No.33
To investigate hydrostatic pressure (HP) effect on myofibrillar protein (Mf) extracted from bovine Semitendinosus muscle, Ca- and Mg-ATPase activities to evaluate denaturation of myosin and actin, and soluble protein contents were observed. In Mf treated with 100 MPa for 5 min was not observed denaturation of myosin and actin. In Mf treated with 200 MPa for 5 min, denaturation of myosin and actin were observed but inactivation rate was low (0.0136 min〈sup〉-1〈/sup〉 ). Inactivation rate of myosin and actin was dramatically increased above 300 MPa treatment. However denaturation of myosin and actin was not that critical with duration time. By increasing pressure size, the amount of myosin and actin in soluble protein eluted in 20 mM potassium phosphate buffer (pH 7.0) containing 0.6 M NaCl were decreased. SDS-PAGE of soluble protein released from Mf suspension in 0.1 M NaCl buffer (pH 7.0) showed that low molecular weight proteins (15~ 36 KDa) were released by HP treatment above 200 MPa. From the results, denaturation of myosin and actin, and release of light molecule proteins of Mf were observed by HP treatment over 200 MPa.