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- 저자 : Kyunglyung Baek (검색결과 3 건)
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Optimization of Plant Structure for Increase Fruit Yield in Tomato (Solanum lycopersicum)
Ji Eun Choi,Hyeonseo Park,Kyunglyung Baek,Hyebhin Eo,Soon Ju Park,Chul Min Kim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Yield is a complex trait that is influenced by several genetic and environmental factors. In terms of environmental factors, tomatoes produce more flowers during low temperatures than at high temperatures, but the effect is insignificant. For many years, breeders have tried to increase the yield and improve the quality of crops by using traditional breeding methods. Today, after much research; we have succeeded in improving crop yield and quality through a genome editing technology called ‘CRISPR/Cas9’. In order to increase the production per unit area of tomatoes, in this experiment, a gene that controls the quantitative trait was used. Because the production had to be increased in a limited area. Quantitative traits work in a variety of ways, some may include- fruit size, quantity, height. Among them, Compound inflorescence (S) and SlERECTA (SlER), which control the quantity and height of crops, were made into transformants using the CRISPR/Cas9. In Arabidopsis thaliana, S, a homologous gene of WUSCHEL-HOMOBOX 9 (WOX9), promotes meristem maturation and regulates tomato inflorescence development. Accordingly, it creates a complex inflorescence with hundreds of flowers. SlER is a homologous gene of Arabidopsis ERECTA. ERECTA is a family gene to which ERECTA-Like1 and ERECTA-Like2 belong, and in particular, encodes leucine-rich repeat receptor-like kinases that control plant above ground organs. Therefore, a phenotype is seen to reduce the height of the crop by targeting and effectively reducing the vegetative growth. We multi-targeted the promoter region to obtain 59 T0 plants in which S and SlER were double edited and 55 T0 plants in which SlER was edited. Since the promoter region was multi-targeted, four randomly selected targets (A, B, C, D) were randomly edited. In order to know that the phenotype occurs according to the promoter sequence, each promoter of 2.2 kb for ‘S’ and 1.8 kb for SlER was sequenced. As a result, ‘S’ and SlER appeared in 6 patterns including biallelic. First, in ‘S’, the number of stem branches increased 10 times compared to WT when A-C parts were removed, and 7 times when A-D parts were removed. When B-D and C-D parts were removed, they came out similar to WT, as 1 or 2 more branches were generated. SlER was similar to or slightly different from WT when the A-D part was missing, and was 0.7 times smaller when the A-C part was missing, and 0.8 times smaller when the B-D part was missing. When only the A target disappeared, the plants were the smallest, 0.5 to 0.6 times that of the WT. In addition, the phenotype of SlER was strong, and it was observed that the smaller the plant, the rounder the leaf. It was found that both S and SlER had a core binding site showing a strong phenotype for the A target among the four targets. In this study, we tried to find out the conditions of plants that are easy to grow in a l imited space and have high yields by controlling the length of stem nodes and the number of inflorescences.
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