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- 저자 : Kyoung min Rheu (검색결과 3 건)
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Kyung-A Byun,Seyeon Oh,Sosorburam Batsukh,Kyoung-Min Rheu,이배진,Kuk Hui Son,Kyunghee Byun 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.6
Sarcopenia is an age-related, progressive skeletal muscle disorder involving the loss of muscle mass and strength. Previous studies have shown that γ-aminobutyric acid (GABA) from fermented oysters aids in regulatory T cells (Tregs) cell expansion and function by enhancing autophagy, and concomitantly mediate muscle regeneration by modulating muscle inflammation and satellite cell function. The fermentation process of oysters not only increases the GABA content but also enhances the content of branched amino acids and free amino acids that aid the level of protein absorption and muscle strength, mass, and repair. In this study, the effect of GABA-enriched fermented sarco oyster extract (FSO) on reduced muscle mass and functions via Treg modulation and enhanced autophagy in aged mice was investigated. Results showed that FSO enhanced the expression of autophagy markers (autophagy-related gene 5 [ATG5] and GABA receptor-associated protein [GABARAP]), forkhead box protein 3 (FoxP3) expression, and levels of anti-inflammatory cytokines (interleukin [IL]-10 and transforming growth factor [TGF]-β) secreted by Tregs while reducing pro-inflammatory cytokine levels (IL-17A and interferon [IFN]-γ). Furthermore, FSO increased the expression of IL-33 and its receptor IL-1 receptor-like 1 (ST2); well-known signaling pathways that increase amphiregulin (Areg) secretion and expression of myogenesis markers (myogenic factor 5, myoblast determination protein 1, and myogenin). Muscle mass and function were also enhanced via FSO. Overall, the current study suggests that FSO increased autophagy, which enhanced Treg accumulation and function, decreased muscle inflammation, and increased satellite cell function for muscle regeneration and therefore could decrease the loss of muscle mass and function with aging.
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Hyun You Chang,Geum Hui Seo,Yong Kuk Lee,Kyoung min Rheu 한국버섯학회 2018 버섯 Vol.22 No.1
Biological efficiency (BE), the ratio of fresh mushrooms harvested per dry substrate weight and expressed as percentage of Lentinula edodes, also known as the shiitake was identified using the Sanjo 701 strain stored in the Department of Mushroom of the Korea National College of Agriculture and Fisheries. The strains were grown in glass columns with varying level of moisture content and different length of mixing periods of 0.5,1,2, and 3 hours. The substrate was sterilized to avoid contamination using the pressure steam Autoclave Sterilizer between normal and high pressure sterilization. Results show that mycelial growth (126mm/15 days) is optimized at 55% moisture level content. Two (2) hours mixing time yields the best mycelial growth of 117mm/15 days. On the other hand, normal pressure sterilization yielded better result with mycelial growth of 96mm/15 days at 100°C sterilization as compare to 88mm /15 days sterilization at 121°C. Mycelial density is higher, 3(+++) at normal pressure sterilization as compare to 2(++) at high pressure sterilization. Further, the sawdust with mixture of 5% woodchips also increased the porosity of the substrate and yielded higher mycelial growth. Thus, at this point we have shown the importance on how to achieve optimum harvest or potential increased yield of shiitake considering its moisture content, mixing time period and substrate porosity.
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ScienceON
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