Quantitative analysis of NaI(Tl) gamma-ray spectrometry using an artificial neural network

Kim, Jinhwan,Lim, Kyung Taek,Kim, Junhyeok,Kim, Chang-jong,Jeon, Byoungil,Park, Kyeongjin,Kim, Giyoon,Kim, Hojik,Cho, Gyuseong Elsevier BV * North-Holland 2019 Nuclear Instruments & Methods in Physics Research. Vol. No.

<P><B>Abstract</B></P> <P>In this manuscript, we propose an algorithm based on an artificial neural network (ANN) for the analysis of the NaI(Tl) gamma-ray spectra with radioisotope (RI) mixtures to identify RIs and determine the relative activity levels of the identified RIs. The ANN was trained based on the spectra that were generated by synthesizing previously identified spectra from single RIs, considering the characteristics of the measurement environments, such as gain shift effects and statistical fluctuations in the spectrum. The proposed ANN was evaluated through several measured spectra that contained up to six certified reference materials for a quantitative analysis. We also evaluated the shift in the spectra due to temperature variations in the range of 0–50 °C and the low-count spectra with a one-second acquisition period. These results were compared with those from an ANN trained through simulated spectra to emphasize the importance of acquiring a high-quality training dataset. In addition, we show that complex low-resolution spectra can be accurately analyzed with the proposed ANN under various scenarios, in which the maximum root mean square error was found to be 2.8%.</P>

Curcumin inhibits hepatitis C virus replication via suppressing the Akt-SREBP-1 pathway

Kim, KyeongJin,Kim, Kook Hwan,Kim, Hye Young,Cho, Hyun Kook,Sakamoto, Naoya,Cheong, JaeHun Elsevier 2010 FEBS letters Vol.584 No.4

<P><B>Abstract</B></P><P>A polyphenolic compound from the curry spice turmeric, curcumin, is known to show anti-viral activity against the influenza virus, adenovirus, coxsackievirus, and the human immunodeficiency virus. However, it remains to be determined whether curcumin can inhibit the replication of hepatitis C virus (HCV). In this study, we showed that curcumin decreases HCV gene expression via suppression of the Akt-SREBP-1 activation, not by NF-κB pathway. The combination of curcumin and IFNα exerted profound inhibitory effects on HCV replication. Collectively, our results indicate that curcumin can suppress HCV replication in vitro and may be potentially useful as novel anti-HCV reagents.</P>

Hepatitis B virus X protein induces lipogenic transcription factor SREBP1 and fatty acid synthase through the activation of nuclear receptor LXRα

Kim, KyeongJin,Kim, Kook Hwan,Kim, Hyeong Hoe,Cheong, JaeHun Portland Press 2008 Biochemical journal Vol.416 No.2

<P>HBV (hepatitis B virus) is a primary cause of chronic liver disease, which frequently results in hepatitis, cirrhosis and ultimately HCC (hepatocellular carcinoma). Recently, we showed that HBx (HBV protein X) expression induces lipid accumulation in hepatic cells mediated by the induction of SREBP1 (sterol-regulatory-element-binding protein 1), a key regulator of lipogenic genes in the liver. However, the molecular mechanisms by which HBx increases SREBP1 expression and transactivation remain to be clearly elucidated. In the present study, we demonstrated that HBx interacts with LXRα (liver X receptor α) and enhances the binding of LXRα to LXRE (LXR-response element), thereby resulting in the up-regulation of SREBP1 and FAS (fatty acid synthase) in the presence or absence of the LXR agonist T0901317 in the hepatic cells and HBx-transgenic mice. Furthermore, HBx also augments the ability to recruit ASC2 (activating signal co-integrator 2), a transcriptional co-activator that controls liver lipid metabolic pathways, to the LXRE with LXRα. These studies place LXRα in a key position within the HBx-induced lipogenic pathways, and suggest a molecular mechanism through which HBV infection can stimulate the SREBP1-mediated control of hepatic lipid accumulation.</P>

Chemokine stromal cell-derived factor-1 induction by C/EBPβ activation is associated with all-<i>trans</i>-retinoic acid-induced leukemic cell differentiation

Kim, KyeongJin,Kim, Hyeong Hoe,Kim, Joon Hong,Choi, Yung Hyun,Kim, Young Hee,Cheong, JaeHun Wiley (John WileySons) 2007 Journal of Leukocyte Biology Vol.82 No.5

Evaluation of Serviceability Performance of Void- Free Asphalt Concrete for Heavy Duty Asphalt Pavement

Injung Kim,Kyeongjin Kim,Sungjin Lee,Kwang W. Kim 한국도로학회 2018 한국도로학회 학술대회 발표논문 초록집 Vol.2018 No.05

There are some places such as bridges in the heavily industrialized area where the pavement should have a strong resistance against heavy axle loading and waterproof function. In those places, many polymer-modified asphalt (PMA) pavements were applied to protect premature cracking, severe rutting and water intrusion without success. Therefore, a much tougher pavement material with waterproofing function was developed for those places. This study evaluated important properties of the special type asphalt mixture which is highly condensed to be almost void-free condition. A high-quality PMA binder with PG82-34 grade was used for preparing the mixture and the optimum binder content was determined to allow near 0% air void in the mix design. The deformation strength(SD) by Kim Test and rut depth by wheel tracking test were measured at 60℃ as high temperature properties. The flexural strength and fracture toughness was measured at -10℃ as low temperature property. The void-free AC showed the higher performance in all four properties than any other asphalt concretes which were prepared for comparison. Therefore, it was shown that the normal concern about limiting air voids within 3-5% was just an apprehension. The void-free AC can be applied for heavy duty pavement on the bridge where the water-proofing function and higher rutting and cracking resistance are required.

A sensory-motor neuron type mediates proprioceptive coordination of steering in <i>C</i> . <i>elegans</i> via two TRPC channels

Yeon, Jihye,Kim, Jinmahn,Kim, Do-Young,Kim, Hyunmin,Kim, Jungha,Du, Eun Jo,Kang, KyeongJin,Lim, Hyun-Ho,Moon, Daewon,Kim, Kyuhyung Public Library of Science 2018 PLoS biology Vol.16 No.6

<▼1><P>Animal locomotion is mediated by a sensory system referred to as proprioception. Defects in the proprioceptive coordination of locomotion result in uncontrolled and inefficient movements. However, the molecular mechanisms underlying proprioception are not fully understood. Here, we identify two transient receptor potential cation (TRPC) channels, <I>trp-1</I> and <I>trp-2</I>, as necessary and sufficient for proprioceptive responses in <I>C</I>. <I>elegans</I> head steering locomotion. Both channels are expressed in the SMDD neurons, which are required and sufficient for head bending, and mediate coordinated head steering by sensing mechanical stretches due to the contraction of head muscle and orchestrating dorsal head muscle contractions. Moreover, the SMDD neurons play dual roles to sense muscle stretch as well as to control muscle contractions. These results demonstrate that distinct locomotion patterns require dynamic and homeostatic modulation of feedback signals between neurons and muscles.</P></▼1><▼2><P><B>Author summary</B></P><P>Proprioception provides the nervous system with feedback about body posture in animals and is essential for the generation of coherent locomotive behaviors, such as walking, running, or crawling. However, little is known about the identity of proprioceptive receptors that sense body movement to regulate locomotion and the extent to which proprioception modulates sensorimotor coordination. Here, we analyze the molecular mechanisms that control head steering locomotion of <I>Caenorhabditis elegans</I>. We show that this movement is regulated by the transient receptor potential cation (TRPC) channels TRP-1 and TRP-2 and the SMDD proprioceptive neurons. We observe that mutant animals for both channels are defective in head steering locomotion and that ectopic expression of TRP-1 or TPR-2 in a <I>C</I>. <I>elegans</I> chemosensory neuron confers head bending–dependent responses, suggesting roles for these channels in proprioception. We also find that SMDD neurons are both necessary and sufficient to generate head steering locomotion via the two channels. Moreover, we demonstrate that the proprioceptive system mediates locomotion coordination by desynchronizing activities in motor systems. We conclude that two TRPC channels in collaboration with the proprioceptive receptor SMDD neurons control head steering in worms during forward locomotion.</P></▼2>

Application of Color Information to Facilitate Finding Books in the Library

Kyeongjin Park,Hyeon Chul Kim,Eun Hye Lee,Kyungdoh Kim 대한인간공학회 2017 大韓人間工學會誌 Vol.36 No.3

Objective: We propose to apply color information to facilitate finding books in the library. Background: Currently, books are classified in the basis of a decimal classification system and a call number in the library. Users find a book using the call number. However, this classification system causes various difficulties. Method: In a process analysis and survey study, we identify what the real problem is and where the problem is occurred. To solve the real problems, we derived a new search method using color information. We conducted a comparative experiment with 48 participants to see whether the new method can show higher performance. Results: The new method using color information showed faster time and higher subjective rating scores than current call number method. Also, the new method showed faster time regardless of the skill level while the call number method showed time differences in terms of the skill level. Conclusion: The effectiveness of the proposed method was verified by experiments. Users will be able to find the desired book without difficulty. This method can improve the quality of service and satisfaction of library use. Application: Our book search method can be applied as a book search tool in a real public library. We hope that the method can provide higher satisfaction to users.

Prolyl hydroxylation primes CMGC kinases for activation through Tyrosine autophosphorylation

KyeongJin Kim,Chung Kwon Kim,Sang Bae Lee 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Hepatitis C virus NS5A protein increases hepatic lipid accumulation via induction of activation and expression of PPARgamma

Kim, KyeongJin,Kim, Kook Hwan,Ha, Eunsin,Park, Jin Young,Sakamoto, Naoya,Cheong, JaeHun Elsevier 2009 FEBS letters Vol.583 No.17

<P><B>Abstract</B></P><P>Steatosis is an established risk factor for disease progression in cases of chronic hepatitis C. Recently it was demonstrated that Hepatitis C virus (HCV) core and non-structural (NS) 2 proteins (NS2) induce lipid accumulation in hepatic cells. However, it has yet to be determined whether other HCV proteins are associated with lipid metabolism. The NS5A augmented the transcriptional activity and gene expression of PPARγ. Furthermore, NS5A increased the ability to recruit the transcriptional coactivator PGC-1s to the PPRE with PPARγ, as well as the interaction with PPARγ2 and PGC-1α. Our results indicate that NS5A may exploit multiple strategies that enhance PPARγ-induced lipid accumulation.</P><P><B>Structured summary</B></P><P>MINT-7229685: <I>PPAR gamma 2</I> (uniprotkb:P37231-2) <I>physically interacts</I> (MI:0914) with <I>PGC1 alpha</I> (uniprotkb:Q9UBK2) by <I>pull down</I> (MI:0096)</P><P>MINT-7229712: <I>PPAR gamma 2</I> (uniprotkb:P37231-2) <I>physically interacts</I> (MI:0914) with <I>NS5A</I> (uniprotkb:P26662) by <I>pull down</I> (MI:0096)</P><P>MINT-7229698: <I>PPAR gamma 2</I> (uniprotkb:P37231-2) <I>physically interacts</I> (MI:0914) with <I>PGC1 alpha</I> (uniprotkb:Q9UBK2) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7229731: <I>PPAR gamma 2</I> (uniprotkb:P37231-2) <I>physically interacts</I> (MI:0914) with <I>NS5A</I> (uniprotkb:P26662) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P>

The orphan nuclear receptor SHP inhibits apoptosis during the monocytic differentiation by inducing p21WAF1

KyeongJin Kim,Yoon Ha Choi,김형회,정재훈 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.6

Small heterodimer partner (SHP) is an atypical member of nuclear receptor superfamily that lacks a DNA-binding domain. In previous study, we showed that SHP, c-jun, p65 of NF-κB subunits, and p21WAF1 expression was increased during monocytic differentiaton with the exposure of human leukemia cells to a differentiation agent, PMA. In this study, c-Jun and p65 were shown to mediate the transcriptional activation of the SHP promoter. In addition, SHP induced the cell cycle regulatory protein levels and cooperatively increased an induction of p21WAF1 expression with p65. Furthermore, SHP protected differentiated cells from etoposide-induced cellular apoptosis through the induction and cytoplasmic sequestration of p21WAF1. Complex formation between SHP and p21WAF1 was demonstrated by means of coimmunoprecipitation. These results suggest that SHP prolongs a cellular survival of differentiating monocytes through the transcriptional regulation of target genes of cell survival and differentiation.