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고지방식이 마우스에서 3,5-dicaffeoylquinic acid의 항비만 효과
강진용(Jin Yong Kang),박선경(Seon Kyeong Park),김종민(Jong Min Kim),박수빈(Su Bin Park),유슬기(Seul Ki Yoo),한혜주(Hye Ju Han),김대옥(Dae Ok Kim),허호진(Ho Jin Heo) 한국식품과학회 2019 한국식품과학회지 Vol.51 No.1
이연구는 고지방식이로 인한 비만 및 대사장애에 대한 3,5-diCQA의 효과를 확인하는 동시에 클로로겐산과의 상대적인 생리 활성을 확인하였다. 클로로겐산은 이전에 보고된 연구결과에서처럼 고지방식이를 섭취하는 쥐의 체중 증가를 효과적으로 억제하였으며, 3,5-diCQA와 비교하였을 때에도 그 효과가 상대적으로 우수하였다. 내장지방의 무게를 측정한 결과 3,5-diCQA와 클로로겐산 모두 내장지방의 축적을 효과적으로 억제하였으며, 이러한 이유는 두 샘플 모두 지방조직에서의 AMPK의 활성화가 증가된 것과 관련이 있는 것으로 보인다. 또한 이 두 샘플은 비만으로 인해 발생하는 산화적 스트레스로부터 간조직을 보호하는 효과가 있었다. 그러나 간조직에서의 지방축적을 확인한 결과에서 클로로겐산은 여전히 간의 지방축적을 억제하는 것으로 확인되었지만 3,5-diCQA는 오히려 간의 지방축적을 증가시킨 것이 확인되었다. 이러한 결과는 3,5-diCQA가 간조직에서 Akt의 활성을 증가시킨 것과 관련이 있는 것으로 보이며 이 증가된 Akt로 인해 3,5-diCQA 그룹의 내당능 개선이 클로로겐산 그룹보다도 우수하였지만 간에서의 지방 축적을 증가시킨 것으로 판단된다. This study was performed to confirm the influence of chlorogenic acid (CGA) and 3,5-dicaffeyolquinic acid (3,5-diCQA) intake on problems caused by high-fat diet. CGA was more effective in suppressing weight gain than 3,5-diCQA. In contrast, 3,5-diCQA was more effective in improving glucose tolerance than CGA. In the biopsy, it was confirmed that CGA inhibited visceral fat and liver fat accumulation. 3,5-diCQA also inhibited visceral fat accumulation, but 3,5-diCQA increased liver fat accumulation. The liver fat accumulation induced oxidative stress, but 3,5-diCQA reduced oxidative damage through its antioxidant activity. The increased liver fat accumulation was because a 3,5-diCQA greatly increased Akt phosphorylation and decreased AMPK phosphorylation in the liver. Consequently, CGA was effective in alleviating the problems caused by high-fat diets, while maintaining normal balance. 3,5-diCQA also showed a positive effect on problems caused by high-fat diets, but it increased liver fat accumulation and thereby had negative consequences.
Seung, Tae Wan,Park, Seon Kyeong,Kang, Jin Yong,Kim, Jong Min,Park, Sang Hyun,Kwon, Bong Seok,Lee, Chang Jun,Kang, Jeong Eun,Kim, Dae Ok,Lee, Uk,Heo, Ho Jin Elsevier 2018 Food Research International Vol.105 No.-
<P>The ameliorating effects of the ethyl acetate fraction from Hibiscus sabdariffa L. (EFHS)(2) against diabetes mellitus (DM)(3) and DM-induced cognitive impairment were investigated on streptozotocin (STZ)(4)-induced DM mice. The EFHS groups showed improved hyperglycemia and glucose tolerance compared to the STZ group. Furthermore, their liver and kidney function and lipid metabolic imbalance in the blood serum were effectively recovered. The EFHS groups significantly ameliorated STZ-induced cognitive impairment in Y-maze, passive avoidance, and Morris water maze (MWM)(6) tests. The EFHS groups showed significant improvement in the antioxidant and cholinergic systems of the brain tissue. In addition, EFHS had an excellent ameliorating effect on protein expression levels from the tau hyperphosphorylation pathways, such as phospho-c-Jun N-terminal kinases (p-JNK),(6) phospho-tau (p-tau),(7) and cleaved poly (ADP-ribose) polymerase (c-PARP).(8) The main compounds of EFHS were identified as various phenolic compounds, including hibiscus acid, caffeoylquinic acid (CQA)(9) isomers, and quercetin derivates. Therefore, EFHS containing various physiologically active materials can potentially be used for improving DM-induced cognitive impairment via its antioxidant activity, improvement of the cholinergic system, and hyperphosphorylation tau signaling.</P>
Lee, Chang Jun,Park, Seon Kyeong,Kang, Jin Yong,Kim, Jong Min,Yoo, Seul Ki,Han, Hye Ju,Kim, Dae-Ok,Heo, Ho Jin ELSEVIER 2019 INDUSTRIAL CROPS AND PRODUCTS Vol. No.
<P><B>Abstract</B></P> <P>Phenolics obtained from plants as natural compounds have been used in cosmetic industry as potential alternatives to synthetic chemicals due to its excellent antioxidant and whitening effects. This study investigated the possibility of using <I>Arctium lappa</I> L. leaf extracts as cosmetic substances by assessing whitening effects through its antioxidant and physiological activity in B16/F10 melanoma cells. The ethyl acetate fraction from <I>Arctium lappa</I> L. leaf showed the highest total phenolics (216.75 mg gallic acid equivalent (GAE)/g) relative to the other fractions (<I>n</I>-hexane, chloroform, and distilled water). The antioxidant activities of EAFA were evaluated based on 2,2′-azino-bis (3-ethyl benzthiazoline-6-sulfonic acid) diammonium salt (ABTS) /α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing/antioxidant power (FRAP), and malondialdehyde (MDA) inhibitory effects. The whitening effects of EAFA were examined using tyrosinase inhibitory activity and inhibition of α-melanocyte stimulating hormone (α-MSH) -induced melanogenesis in B16/F10 cells. The ethyl acetate fraction from <I>Arctium lappa</I> L. leaf effectively inhibited the tyrosinase activity and decreased melanin contents as following α-MSH stimulation. Based on these results, the inhibitory mechanism of melanogenesis was confirmed by measuring phosphorylated c-Jun N-terminal kinase (<I>p</I>-JNK), microphthalmia-associated transcription factor (MITF), tyrosinase-related protein 1 (TRP-1), and tyrosinase in α-MSH–induced B16/F10 cells. The ethyl acetate fraction from <I>Arctium lappa</I> L. leaf downregulated the levels of <I>p</I>-JNK, MITF, TRP-1, and tyrosinase. Finally, the main compounds of EAFA were analyzed using the ultra-performance liquid chromatography-quadrupole-time-of-flight (UPLC Q-TOF) MS system, and the components were identified as follows: trans-5-caffeoylqunic acid, rutin, kaempferol-3-O-rutinoside, 3,5-di-O-caffeoylqunic acid, and 4,5-di-<I>O</I>-caffeoylqunic acid.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ethyl acetate fraction from <I>Arctium lappa</I> L. leaf showed an effective antioxidant activity. </LI> <LI> Ethyl acetate fraction from <I>Arctium lappa</I> L. leaf showed a tyrosinase inhibitory effect. </LI> <LI> Ethyl acetate fraction from <I>Arctium lappa</I> L. leaf indicated a regulatory activity on melanogenesis. </LI> <LI> Major compounds were analyzed as rutin and hydroxycinnamic acid. </LI> </UL> </P>
Kim, Gi-Young,Ko, Woo-Shin,Lee, Jae-Yoon,Lee, Jeong-Ok,Ryu, Chung-Ho,Choi, Byung Tae,Park, Yeong-Min,Jeong, Young-Ki,Lee, Kyeong-Jun,Choi, Kwang-Sik,Heo, Moon-Soo,Choi, Yung Hyun Pharmaceutical Society of Japan 2006 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.29 No.2
<P>Water extract (WE) of <I>Cordyceps militaris</I> has been reported to produce antitumor and immunomodulatory activities <I>in vivo</I> and <I>in vitro</I>. However, the therapeutic mechanism has not been known. In this study, we investigated whether water extract of <I>C. militaris</I> induces the phenotypic and functional maturation of dendritic cells (DC). It profoundly increased CD40, CD54, CD80, CD86, and MHC class II expression in murine bone marrow (BM)-derived myeloid DC. Endocytosis was assessed by the uptake of FITC-dextran and FITC-albumin. The ability of unstimulated DC (UT-DC) to uptake dextran and albumin was higher than that of WE- or LPS-stimulated DC (LPS-DC). Also, UT-DC secreted a low concentration of IL-12, while WE- or LPS-DC secreted higher levels of IL-12 than UT-DC. WE not only formed morphologically mature DC and clusters, but also induced predominantly functional maturation. Moreover, WE is shown to promote the cytotoxicity of specific-cytotoxic T lymphocyte (CTL) induced by DC which were pulsed with P815 tumor-lysate during the stage of antigen presentation. These results suggest that DC maturation by WE can play a critical role in the improvement of the immunoregulatory function in patients with impaired host defense.</P>
Kim, Jong Min,Park, Chang Hyeon,Park, Seon Kyeong,Seung, Tae Wan,Kang, Jin Yong,Ha, Jeong Su,Lee, Du Sang,Lee, Uk,Kim, Dae-Ok,Heo, Ho Jin American Chemical Society, Books and Journals Divi 2017 Journal of agricultural and food chemistry Vol.65 No.13
<P>The ameliorating effects of ginsenoside Re (G Re) on high fat diet (HFD)-induced insulin resistance in CS7BL/6 mice were investigated to assess its physiological function. In the results of behavioral tests, G Re improved cognitive dysfunction in diabetic mice using Y-maze, passive avoidance, and Morris water maze tests. G Re also significantly recovered hyperglycemia and fasting blood glucose level. In the results of serum analysis, G Re decreased triglyceride (TG), total cholesterol (TCHQ), low-density lipoprotein cholesterol (LDLC), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) and :increased the ratio of high-density lipoprotein cholesterol (HDLC). G Re regulated acetylcholine (ACh), acetylcholinesterase (AChE), malondialdehyde (MDA), superoxide dismutase (SOD), and oxidized glutathione (GSH)/total GSH by regtilating the c-Jun N-terminal protein kinase (JNK) pathway. These findings suggest that G Re could be used to improve HFD-induced insulin resistance condition by ameliorating hyperglycemia via protecting the cholinergic and antioxidant systems in the mouse brains.</P>
Jung, Hee-Won,Chung, Chun Kee,Chi, Je G.,Bang, Yung Jue,Heo, Dae Seog,Ahn, Yoon-Ok,Kim, Il Han,Kim, Noe Kyeong,Choi, Kil Soo,Han, Dae Hee 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.9
악성 뇌교종환자에서 수술후 방사선 치료와 함께 시작하여 최소 2주기이상 시행한 ACNU 화학요법이 방사선치료만 시행하였을때보다 관해유지에 부가되는 치료효과가 있는지를 결정하기 위하여 전향성 무작위 임상시험을 시행하였다. 수술후 악성 뇌교종으로 확진된 환자들을 연령, 성별, 수술적출 정도 및 병리 소견으로 충화한 다음, 무작위로 방사선치료 단독군(단독군)(11명) 또는 방사선 및 화학요법 병용군(병용군)(11명)으로 할당하였다. 연령 분포상 50세 이상이 45% 이었고 남녀비는 1 : 1이었다. 전적출은 단독군에서 2례, 병용군에서 5례 시행하였으며 악성 성상세포종이 27%, 다형성교모세포종이 73%이었다. 이러한 연령, 성별, 수술적출의 정도 및 병리소견의 분포는 단독군과 병용군간에 차이가 없었다. 총관해율(완전 및 부분)은 단독군에서 36.4%, 병용군에서 27.3%였다. 환자의 생존기간 및 병의 진행기간을 카프란-마이어법으로 분석하였고 단독군과 병용군 간의 차이는 로그-순위법으로 비교하였다. 일년 누적생존율은 단독군에서 60.6%, 병용군에서 46.7%로 두군간의 차이는 관찰되지 않았다(0.25<p<0.50). 그러나 70주이상의 장기 누적 비진행율은 단독군(22.7%)에서보다 병용군(46.7%)에서 더 높았다. 병용군의 64%에서 부작용(주로 골수억제)이 관찰되었고, 단독군의 36%에서 오심이 관찰되었다. 화학요법제의 용량조절이 필요한 경우가 병용군의 64%에서 관찰되었으나, 골수억제기간이 일시적이었으므로 이로인하여 화학요법의 중단이 필요한 경우는 없었다. 본 초기보고상에서는 ACNU가 종양관해유도 및 관해유지에 통계학적으로 유의한 부가적 효과가 있다고 하기는 어렵다. 그러나, 장기적인 종양관해의 유지에 미치는 부가적 효과에 대한 판정은 환자수가 더 축적되고 추적관찰의 기간이 연장되면 가능할 것으로 판단되었다.
Ha, Jeong Su,Kim, Jong Min,Park, Seon Kyeong,Kang, Jin Yong,Lee, Du Sang,Lee, Uk,Kim, Dae-Ok,Choi, Sung-Gil,Heo, Ho Jin The Royal Society of Chemistry 2018 Food & Function Vol.9 No.6
<P>This study aimed to investigate the ameliorating effect of an ethyl acetate fraction from the fruit <I>Actinidia arguta</I> (EFAA) on amyloid beta (Aβ)-induced neurotoxicity and cognitive deficits in ICR mice. EFAA showed potent protective effects against Aβ-induced neurotoxicity through 2′,7′-dichlorofluorescein diacetate (DCF-DA), 2′,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release into the assay medium. EFAA treatment reduced the intracellular ROS level and lactate dehydrogenase (LDH) release in the mitochondria, and increased cell viability in Aβ-induced neuroblastoma MC-IXC cells. The administration of EFAA significantly attenuated Aβ-induced learning and memory deficits, which were evaluated by Y-maze, passive avoidance, and Morris water maze tests. Furthermore, EFAA showed the ameliorating effect of cholinergic functions by increasing acetylcholine (ACh) levels and decreasing acetylcholinesterase (AChE) activity, and protected antioxidant systems by increasing superoxide dismutase (SOD) and decreasing the oxidized glutathione (GSH)/total GSH and malondialdehyde (MDA) in the brain. Finally, EFAA prevented mitochondrial dysfunction <I>via</I> regulating apoptotic signaling molecules including phosphorylated Akt (p-Akt), phosphorylated tau (p-tau), Bax, and cytochrome c in the brain tissues. Therefore, the present study suggests that EFAA might be a potential source of natural antioxidants with the ability to ameliorate Aβ-induced amnesia.</P>