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Moon, Hyun-Ju,Kim, Kyoung-Nam,Kim, Kwang-Mahn,Choi, Seong-Ho,Kim, Chong-Kwan,Kim, Kee-Deog,LeGeros, Racquel Z.,Lee, Yong-Keun Wiley Publishers 2005 Journal of Biomedical Materials Research Part A Vol. No.
<P>The purpose of this study was to investigate the bone-regenerative effect of calcium phosphate glass in vivo. We prepared amorphous calcium phosphate glass powder having a mean particle size of 400 μm in the system CaO-CaF<SUB>2</SUB>-P<SUB>2</SUB>O<SUB>5</SUB>-MgO-ZnO. Calvarial critical-sized defects (8 mm) were created in 60 male Sprague-Dawley rats. The animals were divided into an experimental group and control group of 30 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As a control, the defect was left empty. The rats were sacrificed 2, 4, or 8 weeks postsurgery, and the results evaluated using radiodensitometric and histological studies; they were also examined histomorphometrically. When the calcium phosphate glass powders with 400-μm particles were grafted, the defects were nearly completely filled with new-formed bone in a clean healing condition after 8 weeks. It was observed that the prepared calcium phosphate glass enhanced new bone formation in the calvarial defect of Sprague-Dawley rats and could be expected to have potential for use as a hard tissue regeneration material. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005</P>
Hyun-Ju Seo,Kwang-Deog Moon,Seon-Min Jeon,Jun-Han Kim,Myung-Sook Choi 한국식품영양과학회 2003 Preventive Nutrition and Food Science Vol.8 No.1
The current study investigated the effect of Korean safflower (Carthamus tinctorius L.) seed powder, its water, ethanol extracts on bone metabolism during recovery from rib-fracture induced by surgical operation in rats. 10-week-old male Sprague-Dawley rats weighing about 320 g were divided into 9 groups after arrival: 10d control (AIN 76 semi-purified diet), 10d safflower seed powder (10d SS-powder), 10d safflower seed ethanol extract (10d SS-EtOH), 10d safflower seed water extract (10d SS-H2O), 20d control (AIN-76 semi-purified diet), 20d safflower seed powder (20d SS-powder), 20d safflower seed ethanol extract (20d SS-EtOH), 20d safflower seed water extract (20d SS-H2O),, 20d sham-operation (20d sham). The dietary level for all the supplements was 5% based on the raw material weight. The rats were fed the experimental diets for 10 days before the rib fracture operation, for a further 10 or 20 days after the operation. A number 9 rib was fractured surgically, a sham-operation also performed. The rats were then sacrificed on the 10th or 20th day after the operation. The body weight initially decreased after the operation in all the rib-fractured groups, then gradually recovered. The concentrations of plasma osteocalcin were higher in the control group than in all the safflower-supplemented groups 10, 20 days after the rib-fracture (p<0.05). The bone-specific ALP (alkaline phosphatase) activity was significantly higher in the SS-EtOH group than in the other groups 20 days after the rib-fracture (p<0.05). The level of urinary DPD (deoxypridinoline) was significantly higher in the SS-EtOH, SS-H₂O groups than in the other groups 10 days after the rib-fracture. When comparing the PTH (parathyroid hormone), calcitonin levels, the SS-H₂O group exhibited the highest PTH level among the groups 10, 20 days after the rib-fracture. Thus, it was concluded that the bone turnover during the fracture-healing period was more rapid in the rats supplemented with safflower seed powder or its fractions than in the control rats. Furthermore, the SS-H₂O fraction was identified as the most effective in stimulating bone remodeling, as bone resorption, bone formation were both significantly increased during fracture healing when compared to the control group.
손태화,문광덕,이병우 한국식생활문화학회 1990 韓國食生活文化學會誌 Vol.5 No.2
건시제조중 invertase의 활성 변화를 조사하고 정제된 invertase의 효소적 특성에 관하여 실험한 바 그 결과는 다음과 같다. 건조가 진행됨에 따라 invertase의 활성은 증가하여 건조 10일째 최고 활성을 나타내었으며 그 이후는 감소하였다. 건시에서 invertase를 250 mM potassium phosphate(pH 7.4)로 추출하여 (NH₄)₂SO₄염석, DEAE-cellulose 및 sephadex G-200 column chromatography의 과정으로 정제한 결과 정제도는 조효소액보다 약 27배 증가되었으며 회수율은 약 11%였다. 최적 pH는 sucrose, raffinose에 대하여 각각 5.0과 6.0으로 나타났으며 최적온도는 40℃였다. 열과 pH 안정성은 50℃까지 그리고 pH 5 부근에서 안정하였다. Sucrose에 대한 K_m값은 2.5 mM이었으며, 정제된 invertase는 polyacrylamide gel 전기영동상에서 하나의 band를 나타내었다. This study was conducted to determine invertase activity in persimmon during the drying process and characterize the purified enzyme. As drying proceeded, invertase activity increased until 10 days and decreased gradually afterwards. Invertase in persimmon fruit was extracted with 250 mM potassium phosphate sulfate buffer at pH 7.4. The enzyme was purified by means of ammonium sulfate fractionation, column chromatography on DEAE-cellulose and gel filtration on Sephadex G-200 column. The optimal temperature of enzyme was 40℃ and optimal pH was 5.0 and 6.0 for sucrose and raffinose, respectively. The enzyme was stable up to 50℃ and pH 3-6. The Km value of the enzyme, with sucrose as a substrate, was 2.5mM. Electrophoretic pattern of purified enzyme solution showed a single band.
밤의 Polyphenol물질과 Polyphenol Oxidase의 생화학적 특성
孫泰華,文廣德,尹基潤 慶北大學校農業科學技術硏究所 1991 慶北大農學誌 Vol.9 No.-
밤과실의 가공 및 저장중 갈변기구를 규명하는 일환으로 밤에 함유된 polyphenol 화합물 및 PPO를 분리, 동정하고 PPO의 생화학적 특성에 대해 연구한 결과는 다음과 같다. 밤의 total phenol 함량은 6.5㎍/g이었고 ferulic acid, caffeic acid, synapic acid, pcoumaric acid, gallic acid, salicylic acid 순으로 함량이 높았다. PPO를 분리, 정제하여 전기영동한 결과 단일의 단백질 및 효소활성밴드를 확인하였으며 정제된 효소의 비활성도는 260.9이고 조효소액보다 11.7배 정제되었다. PPO의 최적 pH와 온도는 각각 5.9, 45℃였으며 효소활성은 80℃에서 15분 처리시 거의 실활되었다. 무기염의 영향을 본 결과 ??, ??, ??들은 효소활성을 증가시켰으나 ??, ??, ??는 활성을 저해시켰다. 저해제로는 L-ascorbic acid, thiourea, sodium chloride, L-cysteine의 저해작용이 강했다. 밤의 PPO는 o-diphenol에 대한 강한 활성을 나타냈으며 특히 catechol에 대해 가장 강했으며 monophenol에는 활성을 나타내지 않았다. PPO의 Km값은 catechol에 대해 5mM이었다. This study was conducted to understand browning characteristics of Chinese Chestnut during processing and storage. For this, the isolation and identification of polyphenolic compounds and the biochemical characteristics of polyphenol oxidase(PPO) were investigated. The content of total phenol was 6.5㎍/g and it was consisted of ferulic acid, caffeic acid, synapic acid, p-coumaric acid, gallic acid and salicylic acid in order. PPO was purified 11.7 fold through ammonium sulfate fractionation, DEAE-cellulose column chromatography and Sephadex G-200 column chromatography. Purified enzyme showed single protein and activity band by polyacrylamide gel electrophoresis. The optimum pH and temperature of PPO were 5.9 and 45℃, respectively. The activity of PPO was lost 93% by exposing at 80℃ for 15minutes. ??, ??, ?? increased the activity of PPO, but ??, ??, ?? inhibited PPO at 10mM concentration. L-ascorbic acid, thiourea, sodium chloride and L-cystein were effective inhibitors of PPO. The activity of PPO was higher for o-diphenols than other polyphenols. The Km value of PPO for catechol was 5mM.
Inheritable Histone H4 Acetylation of Somatic Chromatins in Cloned Embryos
Wee, Gabbine,Koo, Deog-Bon,Song, Bong-Seok,Kim, Ji-Su,Kang, Man-Jong,Moon, Seung-Ju,Kang, Yong-Kook,Lee, Kyung-Kwang,Han, Yong-Mahn American Society for Biochemistry and Molecular Bi 2006 The Journal of biological chemistry Vol.281 No.9