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Important Features for Protein Foldings in Two Acyl Carrier Proteins from Enterococcus faecalis
Yoo Seoyeong,Yeon Jiwon,Kim Eunhee,Kim Yangmee 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.1
The emergence of multi-drug resistant Enterococcus faecalis raises a serious threat to global public health. E. faecalis is a gram-positive intestinal commensal bacterium found in humans. E. faecalis can endure extreme environments such as high temperature, pressure, and high salt, which facilitates them to cause infection in hospitals. E. faecalis has two acyl carrier proteins, AcpA (EfAcpA) in de novo fatty acid synthesis (FAS) and AcpB (EfAcpB) which utilizes exogenous fatty acids. Previously, we determined the tertiary structures of these two ACPs and investigated their structure-function relationships. Solution structures revealed that overall folding of these two ACPs is similar to those of other bacterial ACPs. However, circular dichroism (CD) experiments showed that the melting temperature of EfAcpA is 76.3°C and that of EfAcpB is 79.2°C, which are much higher than those of other bacterial ACPs. In this study, to understand the origin of their structural stabilities, we verified the important residues for stable folding of these two ACPs by monitoring thermal and chemical denaturation. Hydrogen/deuterium exchange and chemical denaturation experiments on wild-type and mutant proteins revealed that Ile10 of EfAcpA and Ile14 of EfAcpB mediate compact intramolecular packing and promote high thermostability and stable folding. E. faecalis may maximize efficiency of FAS and increase adaptability to the environmental stress by having two thermostable ACPs. This study may provide insight into bacterial adaptability and development of antibiotics against multi-drug-resistant E. faecalis.
패션 큐레이션의 서비스 품질이 사용자 만족, 신뢰, 지속사용의도에 미치는 영향
김서영 ( Seoyeong Kim ),김은혜 ( Eunhye Kim ),이진화 ( Jin Hwa Lee ) 한국의류산업학회 2020 한국의류산업학회지 Vol.22 No.6
This study tried to investigate the effect of the service quality attributes of the curation on the satisfaction, trust, and intention to continuous use of consumers in that curation services are derived in various forms and being greatly activated, there are not many academic discussions centered on them. Besides, differences between curation services by each service provider were also verified in this study. Data collection was conducted for one month in August 2019, with 373 men and women in their 20s. The results of the study are as follows. First, according to the result of investigating the variables that make effect on user satisfaction of curation service, it was identified that satisfaction has increased when figures of fulfillment, responsiveness, personalization, design, ease of use, and safety were higher. Second, among the variables that influence the reliability of the curation service, trust increases when user satisfaction, design, ease of use, and safety are higher. Third, satisfaction has a positive effect on trust, and both satisfaction and reliability affect the intention to continue use. Fourth, as a result of examining the difference in quality between the curation services divided by Curating subject, it was found that satisfaction, ubiquitous connectivity, and responsive quality were measured higher in the business operator service than in the user curation service.
인공지능 기반 시선 추적 기법을 활용한 모바일 커머스 사용자 시선 데이터 분석
김서영(Seoyeong Kim),장지인(Jiin Jang),박태정(Taejung Park) 한국디지털콘텐츠학회 2023 한국디지털콘텐츠학회논문지 Vol.24 No.5
In this study, we implemented a system where the user gaze data were collected while they engaged in mobile commerce, and these data were analyzed based on the area of interest. The system is based on a technology that enables gaze tracking with general mobile devices and artificial intelligence without dedicated hardware for eye tracking. We conducted experiments to prove that the results can be expressed as quantitative indicators for consumer analysis and intuitive information visualization. The proposed method involves pre-processing of gaze data, classification according to the gaze characteristics, processing of interest-specific regions of gazed points, and information dimension. In addition, the proposed method is accessible at a low cost compared with existing methods, which require purchasing, installation, and use of expensive hardware. The proposed method allows a large number of users with general smartphones or tablets to use the mobile commerce environment naturally without time and space limitations in gathering target information. In conclusion, our method can collect and analyze more specific gaze information-based data for large-scale purchasing processes that are previously impossible.
『전운옥편(全韻玉篇)』에 나타난 정·속음의 성격 고찰 상(上) ― 『동국정운(東國正韻)』 음운 체계와의 비교를 통하여
김은희 ( Kim Eunhee ),김민경 ( Kim Mingyeong ),김서영 ( Kim Seoyeong ) 한국중국언어학회 2020 중국언어연구 Vol.0 No.86
It is common knowledge that Chinese characters have three elements of form, sound and meaning. In the past, Korea has imported and utilized these Chinese characters since ancient times. However, the way the Chinese pronounce these words and the Koreans pronounce these characters are drastically different due to the different phonological system that exist between the two countries. It has been actually been proven that the traditional Korean reading sounds of the Chinese characters derive from the Chinese traditional pronunciation guide dictionary, the Yunshu(韻書). The “Jeon-un okpyun(『全韻玉篇』)” which is a Sino-Korean dictionary actually covers about 620 Chinese characters with attachments of both the correct sounds and popular reading sounds. What is interesting is that when the most common reading sounds recorded in this dictionary were compared with the ones in yunshu, they could not be found in the Fanqie(反切: Traditional Chinese lexicography) of the yunshu. Therefore, based on some of the findings including the above fact, it may be possible to draw a conclusion that the popular reading sounds in the “Jeon-un okpyun“ are sounds which have been created by the Koreans themselves.