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Jina SON,Kei-Anne BARITUGO,Yu Jung SOHN,Kyoung Hee KANG,Hee Taek KIM,Jeong Chan JOO,Si Jae PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Gamma-aminobutyrate (GABA) is a valuable amino-carboxylic acid that is used in food and pharmaceutical industry. Biosynthesis of GABA involves the decarboxylation of l-glutamate using glutamate decarboxylase (GAD). Thus, C. glutamicum with heterologous expression of GAD has been used for direct fermentative production of GABA from glucose. However, GABA production is not efficient since there is a significant difference between optimal pH for GAD activity (pH 4.0) and cell growth (pH 7.0). Thus, we aimed to develop recombinant C. glutamicum strains with expression of GAD from various microorganisms and compared their ability to produce GABA at near neutral pH. Here, we will present the development of metabolically engineered C. glutamicum strains for one-step fermentative GABA production from glucose at optimally balanced neutral pH.
Recent Advances in Systems Metabolic Engineering Strategies for the Production of Biopolymers
손유정,김희택,조서영,송혜민,Kei-Anne Baritugo,표지원,최종일,주정찬,박시재 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.6
Biopolymers consisting of at least one monomer, which are produced from renewable carbon sources, are being highly sought out since ubiquitous plastics are mainly produced from petrochemical processes causing severe environmental pollution. Therefore, the development of microbial cell factories, which can efficiently synthesize diverse types of monomers and polymers, is also becoming increasingly important. The applicability of traditional metabolic engineering strategies has extended with the combination of systems biology, synthetic biology, and evolutionary engineering in a systemic and versatile manner, and are collectively termed as systems metabolic engineering. Accordingly, recent advances in biotechnology have paved the way for enabling the production of an increasing number of monomers and polymers by providing several tools and strategies associated with systems metabolic engineering. In this review, we have focused on the substantial efforts made on the development of different approaches of systems metabolic engineering, particularly based on synthetic biology and evolutionary engineering, for the efficient production of monomers and polymers.