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Oh, Jisun,Daniels, Gabrielle J,Chiou, Lawrence S,Ye, Eun-Ah,Jeong, Yong-Seob,Sakaguchi, Donald S Wiley 2014 Biotechnology Journal Vol.9 No.7
<P>Adult hippocampal progenitor cells (AHPCs) are generally maintained as a dispersed monolayer population of multipotent neural progenitors. To better understand cell-cell interactions among neural progenitors and their influences on cellular characteristics, we generated free-floating cellular aggregates, or neurospheres, from the adherent monolayer population of AHPCs. Results from in vitro analyses demonstrated that both populations of AHPCs were highly proliferative under maintenance conditions, but AHPCs formed in neurospheres favored differentiation along a glial lineage and displayed greater migrational activity than the traditionally cultured AHPCs. To study the plasticity of AHPCs from both populations in vivo, we transplanted green fluorescent protein (GFP)-expressing AHPCs via intraocular injection into the developing rat eyes. Both AHPC populations were capable of surviving and integrating into developing host central nervous system, but considerably more GFP-positive cells were observed in the retinas transplanted with neurosphere AHPCs, compared to adherent AHPCs. These results suggest that the culture configuration during maintenance for neural progenitor cells (NPCs) influences cell fate and motility in vitro as well as in vivo. Our findings have implication for understanding different cellular characteristics of NPCs according to distinct intercellular architectures and for developing cell-based therapeutic strategies using lineage-committed NPCs.</P>
Oh, Jisun,Yoon, Hyo-Jin,Jang, Jeong-Hoon,Kim, Do-Hee,Surh, Young-Joon The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3
Background: The ginsenoside Rg3, one of active components of red ginseng, has chemopreventive and anticancer potential. Cancer stem cells retain self-renewal properties which account for cancer recurrence and resistance to anticancer therapy. In our present study, we investigated whether the standardized Korean Red Ginseng extract (RGE) and Rg3 could modulate the manifestation of breast cancer stem cell-like features through regulation of self-renewal activity. Methods: The effects of RGE and Rg3 on the proportion of $CD44^{high}/CD24^{low}$ cells, as representative characteristics of stem-like breast cancer cells, were determined by flow cytometry. The mammosphere formation assay was performed to assess self-renewal capacities of breast cancer cells. Aldehyde dehydrogenase activity of MCF-7 mammospheres was measured by the ALDEFLUOR assay. The expression levels of Sox-2, Bmi-1, and P-Akt and the nuclear localization of hypoxia inducible $factor-1{\alpha}$ in MCF-7 mammospheres were verified by immunoblot analysis. Results: Both RGE and Rg3 decreased the viability of breast cancer cells and significantly reduced the populations of $CD44^{high}/CD24^{low}$ in MDA-MB-231 cells. RGE and Rg3 treatment attenuated the expression of Sox-2 and Bmi-1 by inhibiting the nuclear localization of hypoxia inducible $factor-1{\alpha}$ in MCF-7 mammospheres. Suppression of the manifestation of breast cancer stem cell-like properties by Rg3 was mediated through the blockade of Akt-mediated self-renewal signaling. Conclusion: This study suggests that Rg3 has a therapeutic potential targeting breast cancer stem cells.
Oh, Jisun,Kim, Jungeun,Jang, Jin Ho,Lee, Sangwoo,Park, Chul Min,Kim, Woo-Keun,Kim, Jong-Sang MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.4
<P>The present study aimed to evaluate the anti-melanogenic activity of 1,6-diphenyl-1,3,5-hexatriene and its derivatives in B16F10 murine melanoma cells and zebrafish embryos. Twenty five (1<I>E</I>,3<I>E</I>,5<I>E</I>)-1,6-<I>bis</I>(substituted phenyl)hexa-1,3,5-triene analogs were synthesized and their non-cytotoxic effects were predictively analyzed using three-dimensional quantitative structure-activity relationship approach. Inhibitory activities of these synthetic compounds against melanin synthesis were determined by evaluating melanin content and melanogenic regulatory enzyme expression in B16F10 cells. The anti-melanogenic activity was verified by observing body pigmentation in zebrafishes treated with these compounds. Compound <B>#2</B>, <B>#4</B>, and <B>#6</B> effectively decreased melanogenesis induced by α-melanocyte-stimulating hormone. In particular, compound <B>#2</B> remarkably lowered the mRNA and protein expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), and TYRP2 in B16F10 cells and substantially reduced skin pigmentation in the developed larvae of zebrafish. These findings suggest that compound <B>#2</B> may be used as an anti-melanogenic agent for cosmetic purpose.</P>
Jisun Oh,Hyo-Jin Yoon,Jeong-Hoon Jang,Do-Hee Kim,Young-Joon Surh 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.3
Background: The ginsenoside Rg3, one of active components of red ginseng, has chemopreventive and anticancer potential. Cancer stem cells retain self-renewal properties which account for cancer recurrence and resistance to anticancer therapy. In our present study, we investigated whether the standardized Korean Red Ginseng extract (RGE) and Rg3 could modulate the manifestation of breast cancer stem cellelike features through regulation of self-renewal activity. Methods: The effects of RGE and Rg3 on the proportion of CD44<SUP>high</SUP>/CD24<SUP>low</SUP> cells, as representative characteristics of stem-like breast cancer cells, were determined by flow cytometry. The mammosphere formation assay was performed to assess self-renewal capacities of breast cancer cells. Aldehyde dehydrogenase activity of MCF-7 mammospheres was measured by the ALDEFLUOR assay. The expression levels of Sox-2, Bmi-1, and P-Akt and the nuclear localization of hypoxia inducible factor-1α in MCF-7 mammospheres were verified by immunoblot analysis. Results: Both RGE and Rg3 decreased the viability of breast cancer cells and significantly reduced the populations of CD44<SUP>high</SUP>/CD24<SUP>low</SUP> in MDA-MB-231 cells. RGE and Rg3 treatment attenuated the expression of Sox-2 and Bmi-1 by inhibiting the nuclear localization of hypoxia inducible factor-1α in MCF-7 mammospheres. Suppression of the manifestation of breast cancer stem cellelike properties by Rg3 was mediated through the blockade of Akt-mediated self-renewal signaling. Conclusion: This study suggests that Rg3 has a therapeutic potential targeting breast cancer stem cells.
Chae, Oh B.,Kim, Jisun,Park, Inchul,Jeong, Hyejeong,Ku, Jun H.,Ryu, Ji Heon,Kang, Kisuk,Oh, Seung M. American Chemical Society 2014 Chemistry of materials Vol.26 No.20
<P>A vanadium pentoxide electrode is prepared in the amorphous form (<I>a</I>-V<SUB>2</SUB>O<SUB>5</SUB>), and its electrode performances are compared to those for its crystalline counterpart (<I>c-</I>V<SUB>2</SUB>O<SUB>5</SUB>). The <I>a</I>-V<SUB>2</SUB>O<SUB>5</SUB> electrode outperforms <I>c-</I>V<SUB>2</SUB>O<SUB>5</SUB> in several ways. First, it is free from irreversible phase transitions and Li trapping, which evolve in <I>c</I>-V<SUB>2</SUB>O<SUB>5</SUB>, probably due to the lack of interactions between the inserted Li<SUP>+</SUP> ions/electrons and V<SUB>2</SUB>O<SUB>5</SUB> matrix. Second, the absence of Li trapping allows a reversible capacity amounting to >600 mA h g<SUP>–1</SUP>, which is larger than that given by <I>c-</I>V<SUB>2</SUB>O<SUB>5</SUB>. Third, it shows an excellent rate property. The notably high reversible capacity and rate capability seem to be due to Li storage at vacant sites that are ill-defined but numerous in <I>a</I>-V<SUB>2</SUB>O<SUB>5</SUB>, which Li<SUP>+</SUP> ions can easily access. However, irreversible capacity of <I>a</I>-V<SUB>2</SUB>O<SUB>5</SUB> is appreciable in the first cycle due to a parasitic Li reaction with surface hydroxyl groups. Treatment with <I>n</I>-butyllithium can suppress the irreversible capacity by removing the surface hydroxyl groups.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2014/cmatex.2014.26.issue-20/cm502268u/production/images/medium/cm-2014-02268u_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm502268u'>ACS Electronic Supporting Info</A></P>