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        Multidrug Resistance Mechanisms of Carbapenem Resistant Klebsiella pneumoniae Strains Isolated in Chongqing, China

        Jinrong Yan,Shuli Pu,Xiaojiong Jia,XiuYu Xu,Shuangshuang Yang,Jing Shi,Shan Sun,Liping Zhang 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.5

        Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is considered a serious global threat. However, little is known regarding the multidrug resistance (MDR) mechanisms of CRKP. This study investigated the phenotypes and MDR mechanisms of CRKP and identified their clonal characteristics. Methods: PCR and sequencing were utilized to identify antibiotic resistance determinants. Integron gene cassette arrays were determined by restriction fragment length polymorphism (RFLP) analysis. Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used for epidemiological analysis. Plasmids were typed by using a PCR-based replicon typing and analyzed by conjugation and transformation assays. Results: Seventy-eight strains were identified as resistant to at least one carbapenem; these CRKP strains had a high prevalence rate (38.5%, 30/78) of carbapenemase producers. Additionally, most isolates harbored MDR genes, including Extended spectrum β-lactamases (ESBLs), AmpC, and quinolone and aminoglycoside resistance genes. Loss of porin genes was observed, and Class 1 integron was detected in 66.7% of the investigated isolates. PFGE and MLST results excluded the occurrence of clonal dissemination among these isolates. Conclusions: A high prevalence of NDM-1 genes encoding carbapenem resistance determinants was demonstrated among the K. pneumoniae isolates. Importantly, this is the first report of blaNDM-1 carriage in a K. pneumoniae ST1383 clone in China and of a MDR CRKP isolate co-harboring blaNDM-1, blaKPC-2, blaCTX-M, blaSHV, acc(6’)-Ib, rmtB, qnrB, and acc(6’)-Ib-cr.

      • A SDR Prototype for Backscatter Sensor Networks

        Jinrong Mo,Shengbo Hu,Feng Lv,Yanfeng Shi,Tingting Yan 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.10

        Low cost and low power is one of the important reasons in the wide application of backscatter sensor networks. Based on LabVIEW and USRP platforms, this paper presents and implements a simple and configurable SDR prototype for backscatter sensor networks. The SDR prototype can apply to the multi-sensor environment with low power. The prototype consists of configurable backscatter transceiver with homodyne receiver and sensor nodes. It can reduce the power consumption of system efficiently through harvesting wireless RF energy and waking sensor nodes. The frequency division multiple access method with configurable sub-carrier is proposed, and the constraint conditions of sensing data pulse period for avoiding collision among sensors are presented and proved in this paper. The performance of the prototype is analyzed, and the closed-form solution of the probability of outage under the log-normal channel is also given and proved. Finally, the experiment and simulation show the SDR prototype is effective and feasible. The EVM value is less than 2.8%, and the probability of outage error between numerical analysis and Monte Carlo simulation is less than 1.86%.

      • Nonlegumes Respond to Rhizobial Nod Factors by Suppressing the Innate Immune Response

        Liang, Yan,Cao, Yangrong,Tanaka, Kiwamu,Thibivilliers, Sandra,Wan, Jinrong,Choi, Jeongmin,Kang, Chang ho,Qiu, Jing,Stacey, Gary American Association for the Advancement of Scienc 2013 Science Vol.341 No.6152

        <P><B>Stealth Nod Factor Recognition</B></P><P>Legumes' symbiotic interaction with nitrogen fixing bacteria supplies the plant with nitrogen. Many important crop plants, however, cannot establish these symbioses and, thus, agriculture depends on externally applied fertilizers. Surprisingly, <B>Liang <I>et al.</I></B> (p. 1384, published online 5 September) found that several nonleguminous plants, including <I>Arabidopsis</I>, tomato, and corn, were able to respond to the same Nod factors that initiate the microbial symbiosis in soybean.</P>

      • KCI등재

        Evaluation of Six Phenotypic Methods for the Detection of Carbapenemases in Gram-Negative Bacteria With Characterized Resistance Mechanisms

        Kunling Sun,XiuYu Xu,Jinrong Yan,Liping Zhang 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.4

        Background: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. Methods: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains. The collection included 117 non-carbapenemase, 18 Klebsiella pneumoniae carbapenemases (KPC) producers, 46 New Delhi metallo-β-lactamases (NDM) producers, 11 imipenemases (IMP) producers, and 51 oxacillinases (OXA) producers, and 13 strains harboring two different carbapenemase genes. Results: The specificity of the THT (91.5%) was significantly lower than other methods, each of which had 100% specificity (P<0.003). This can be attributed to the false detection of Ampler class C β-lactamases (AmpC) carriers. The CNPt-direct and CIM yielded the highest sensitivities (P<0.003), which were comparable (92.8% vs 93.5%, P>0.999). Because of improved detection of NDM carriers, THT showed significantly higher sensitivity than the MHT (84.9% vs 75.5%, P<0.001). However, poor performances in detecting OXA still influenced the sensitivities of the CNPt (66.2%) and BCT (82.0%), as well as the MHT and THT. Conclusions: CNPt-direct and CIM demonstrated the best performance for the efficient detection of carbapenemase among the six evaluated methods. Except the MHT and THT, the detection of carbapenemase-producing Enterobacteriaceae by all the other methods was acceptable, when the OXA-type carbapenemase was not prevalent.

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