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Jie Wu,Zefu Wu 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7
We integrate institutional theory and the resource-based view of capability to examine the interplay of institutional environments and firm-specific capabilities on value-creating synergy by emerging market firms (EMFs). We argue that EMFs expand into countries with better-developed institutions as a means to overcome poor institutions at home. EMFs that develop strong absorptive capacity and knowledge transfer capability are better able to benefit from a portfolio of complementary sources of knowledge across multiple markets. We analyze data on a sample of manufacturing firms’ international expansion to demonstrate that expansion into a host country with a better-developed institutional environment promotes innovation success. This effect was found to be stronger for firms with strong absorptive capacity and well-developed knowledge transfer capability.
( Wu Jie Su ),( Yan Wu ),( Hui Ling Wu ),( Wen Bing Wang ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.15 No.2
Open reading frame 4 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm4, is a gene whose function is completely unknown. With the recently developed BmNPV bacmid and a modified pFastBac1 whose polyhedrin promoter was replaced with ie1 promoter, a recombinant bacmid expressing Bm4-EGFP fusion protein under the control of ie1 promoter in BmN cells was successfully constructed. The result not only showed that the polyhedrin promoter can be replaced efficiently with other promoters to direct the expression of foreign gene in BmN cells by using Bac-to-Bac/BmNPV baculovirus expression system but also laid the foundation for rescue experiment of Bm4 deletion mutant due to the ability of ie1 promoter to direct gene expression throughout the infection cycle.
Novel Phage Display-Derived H5N1-Specific scFvs with Potential Use in Rapid Avian Flu Diagnosis
( Jie Wu ),( Xian Qiao Zeng ),( Hong Bin Zhang ),( Han Zhong Ni ),( Lei Pei ),( Li Rong Zou ),( Li Jun Liang ),( Xin Zhang ),( Jin Yan Lin ),( Chang Wen Ke ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5
The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.
Jie Wu,Jing Yin,Baixing Zhuang,Mingjie Wang,Jitao Zhang 한국자기학회 2019 Journal of Magnetics Vol.24 No.4
At present, almost all hybrid excitation machines (HEMs) apply DC field current to control the air-gap flux. However, the DC field control mode results in a problem that the capability of field-weakening is not equal to that of field-strengthening. This paper briefly explains the mechanism of asymmetric bidirectional field control capability in present HEMs, proposes a consequent pole hybrid excitation synchronous (CPHES) machine with AC field control mode to solve the asymmetric problem. The structure and principles of CPHES machine are presented. Additionally, the equivalent magnetic circuit model of CPHES machine is derived to reduce the computational complexity of analyzing CPHES structure by using three-dimensional finite element analysis (3DFEA). The proposed equivalent model and 3D-FEA are compared, the results verify the proposed model is basically consistent with the 3D-FEA, and the proposed model is able to reduce the computational complexity greatly.
Jie, Wu 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
A new method of design of the highpass filter using OTA-C is presented. The method is based on the mixed integrator and nodal voltage simulation for the passive LC ladder network. The result structure has electronic tunable ability with a single current.
Incidence and Mortality of Female Breast Cancer in Jiangsu, China
Wu, Li-Zhu,Han, Ren-Qiang,Zhou, Jin-Yi,Yang, Jie,Dong, Mei-Hua,Qian, Yun,Wu, Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6
Objectives: The aim of this study was to describe and analyze the incidence and mortality of female breast cancer in Jiangsu Province of China. Methods: Incidence and mortality data for female breast cancer and corresponding population statistics from eligible cancer registries in Jiangsu from 2006 to 2010 were collected and analyzed. Crude rates, age-specific rates and age-standardized rates of incidence and mortality were calculated, and annual present changes (APCs) were estimated to describe the time trends. Results: From 2006 to 2010, 11,013 new cases and 3,068 deaths of female breast cancer were identified in selected cancer registry areas of Jiangsu. The annual average crude incidence and age-standardized incidence by world population (ASW) were 25.2/ and 17.9/100,000 respectively. The annual average crude and ASW for mortality rates were 7.03/ and 4.81/100,000. The incidence was higher in urban areas than that in rural areas, and this was consistent in all age groups. No significant difference was observed in mortality between urban and rural areas. Two peaks were observed when looking at age-specific rates, one at 50-59 years and another at over 85 years. During the 5 years, incidence and mortality increased with APCs of 4.47% and 6.89%, respectively. Compared to the national level, Jiangsu is an area with relatively low risk of female breast cancer. Conclusion: Breast cancer has become a main public health problem among Chinese females. More prevention and control activities should be conducted to reduce the burden of this disease, even in relatively low risk areas like Jiangsu.
Wu, Di,Su, Liang-Cheng,Wu, Xiao-Jie,Zhao, Guo-Dong The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.2
An optimized space vector pulse width modulation (SVPWM) method with common mode voltage elimination and neutral point potential balancing is proposed for an open-end winding induction motor. The motor is fed from both of the ends with two neutral point clamped (NPC) three-level inverters. In order to eliminate the common mode voltage of the motor ends and balance the neutral point potential of the DC link, only zero common mode voltage vectors are used and a balancing control factor is gained from calculation in the strategy. In order to improve the harmonic characteristics of the output voltages and currents, the balancing control factor is regulated properly and the theoretical analysis is provided. Simulation and experimental results show that by adopting the proposed method, the common mode voltage can be completely eliminated, the neutral point potential can be accurately balanced and the harmonic performance for the output voltages and currents can be effectively improved.
Application of Dynamic Regulation to Increase L-Phenylalanine Production in Escherichia coli
( Jie Wu ),( Yongfei Liu ),( Sheng Zhao ),( Jibin Sun ),( Zhaoxia Jin ),( Dawei Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.6
Current strategies of strain improvement processes are mainly focused on enhancing the synthetic pathways of the products. However, excessive metabolic flux often creates metabolic imbalances, which lead to growth retardation and ultimately limit the yield of the product. To solve this problem, we applied a dynamic regulation strategy to produce L-phenylalanine (LPhe) in Escherichia coli. First, we constructed a series of Phe-induced promoters that exhibited different strengths through modification of the promoter region of tyrP. Then, two engineered promoters were separately introduced into a Phe-producing strain xllp1 to dynamically control the expression level of one pathway enzyme AroK. Batch fermentation results of the strain xllp3 showed that the titer of Phe reached 61.3 g/l at 48 h, representing a titer of 1.36- fold of the strain xllp1 (45.0 g/l). Moreover, the L-Phe yields on glucose of xllp3 (0.22 g/g) were also greatly improved, with an increase of 1.22-fold in comparison with the xllp1 (0.18 g/ g). In summary, we successfully improved the titer of Phe by using dynamic regulation of one key enzyme and this strategy can be applied for improving the performance of strains producing other aromatic amino acids and derived compounds.
Simulating the construction process of steel-concrete composite bridges
Jie Wu,Dan M. Frangopol,Mohamed Soliman 국제구조공학회 2015 Steel and Composite Structures, An International J Vol.18 No.5
This paper presents a master-slave constraint method, which may substitute the conventional transformed-section method, to account for the changes in cross-sectional properties of composite members during construction and to investigate the time-dependent performance of steel-concrete composite bridges. The time-dependent effects caused by creep and shrinkage of concrete are considered by combining the age-adjusted effective modulus method and finite element analysis. An efficient computational tool which runs in AutoCAD environment is developed to simulate the construction process of steel-concrete composite bridges. The major highlight of the developed tool consists in a very convenient and user-friendly interface integrated in AutoCAD environment. The accuracy of the proposed method is verified by comparing its results with those provided by using the transformed-section method. Furthermore, the computational efficiency of the developed tool is demonstrated by applying it to a steel-concrete composite bridge.