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- 저자 : Jiandong Cui (검색결과 4 건)
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Ying Hou,Fengping Wang,Zhilei Tan,Jiandong Cui,Shiru Jia 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.3
ε-Poly-L-lysine (ε-PL) is a natural antimicrobial cationic peptide. Antimicrobial activity of ε-PL is closely related to its molecular weight (Mw). However, the antimicrobial mechanisms of ε-PL with different Mws are still vague. In this study, Saccharomyces cerevisiae was used as the model system to analyze the mechanism from these three aspects: cell wall, cell membrane, and metabolism. The results showed that high-Mw ε-PL (1-3 kDa and >3 kDa) and commercial ε-PL product caused cell wall lesions, and significantly improved cell membrane permeability compared to low-Mw ε-PL (<1 kDa), resulting in leaking of the protoplasm through the pores and cell death. Furthermore, metabolomics analysis showed that high-Mw ε-PL (1-3 kDa and >3 kDa) and product displayed higher inhibition effect on the glycolysis pathway and tricarboxylic cycle than that of low-Mw ε-PL (<1 kDa).
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Design and bio-applications of biological metal-organic frameworks
Baoting Sun,Muhammad Bilal,Shiru Jia,Yunhong Jiang,Jiandong Cui 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.12
Biological metal-organic frameworks (bioMOFs) are a new subclass of the MOF family. In comparison with traditional MOFs, the bioMOFs are made of multifunctional biologically related ligands (bio-ligand) and metal ions. The bio-ligands confer biological compatibility for traditional MOFs, thus providing many opportunities for a wide array of biological applications. This review highlights the recent advances in the synthesis of bioMOFs comprising multifunctional bio-ligands and metal ions. These bio-ligands include nucleobases, amino acids, peptides, proteins, cyclodextrin, saccharides, and other biomolecules. Furthermore, the potential bio-applications of bioMOFs in several fields such as biomedicine, biosensing and bioimaging, antimicrobial applications, biomimetic catalysis, chiral separation, and environmental protection are also demonstrated.
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Efficient Immobilization of Enzymes on Amino Functionalized MIL-125-NH2 Metal Organic Framework
Zichen Wang,Yang Liu,Jinhong Li,Guoqing Meng,Daoyu Zhu,Jiandong Cui,Shi Ru Jia 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.1
As a series of metal organic framework (MOFs), materials of institute lavoisier frameworks (MILs) are expected to be excellent supports for enzyme immobilization due to their good water-stability and acid tolerance. However, enzyme loading on MIL-125-NH2 is very low due to small pore size and few amino groups on the surface of the MIL-125-NH2. In this work, catalase (CAT) was immobilized on the MIL-125-NH2 and amino functionalized MIL-125-NH2 by adsorption (CAT@MIL-125-NH2) and covalent binding (CAT@Amino MIL-125-NH2), respectively. Compared with the CAT@MIL-125-NH2 and free CAT, the CAT@Amino MIL-125-NH2 displayed high activity recovery, good pH stability, stability against denaturants, and thermostability. Furthermore, activity recovery of CAT@Amino MIL-125-NH2 was 56% higher than CAT@MIL-125-NH2. The CAT@Amino MIL-125-NH2 still retained 50% residual activity for 14 days at room temperature, whereas free CAT lost activity after storage for 1 day at the same storage conditions. Furthermore, the CAT@Amino MIL-125-NH2 maintained 62% of its initial activity after 4 consecutive uses, showing good reusability. The results showed that the amino functionalized MIL- 125-NH2 is an excellent carrier of enzyme immobilization.
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Hongjie Bian,Gaoyang Wang,Mengfei Cao,Ziyuan Wang,Jiandong Cui 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.6
Polyvinyl alcohol (PVA) is a synthetic polymer that is difficult to degrade in nature. In this study, we synthesized PVA-degrading enzymes (PVAase)-Cu3(PO4)2 hybrid nanoflowers by using crude PVAase from Bacillus niacini for PVA degradation. The influences of PVAase concentration, Cu2+ concentration, and incubation time on the nucleation and activity of the PVAase hybrid nanoflower were investigated. The maximal activity recovery of the PVAase hybrid nanoflower was approximately 85% at 0.25 mg/mL of PVAase, 0.36mM Cu2+, and 72 h incubation time. The optimum temperature and pH of PVAase did not change before and after immobilization. Compared with free PVAase, the PVAase hybrid nanoflower showed high thermal stability and storage stability. Additionally, the PVAase hybrid nanoflower displayed excellent reusability after eight cycles and promising PVA degradability, indicating its potential application in PVA degradation.
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