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고추 탄저병 저항성 계통육성을 위한 종간교잡 및 배배양
박효근 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-
Hot pepper is one of the most important vegetable crops in Korea. Pepper production, however, has been severely damaged by several destructive diseases, of which anthrac- nose caused by Colletotrichum gloeosporioides is the most serious one. We and other researchers in Korea and abroad were not able to identify good resistant source from Capsicum annuum, the only cultivated species in Korea, despite of many attempts. Fortunately we obtained very strong resistant germplasm, which is belong to Capsicum baccatum from AVRDC. Since we could not find any significantly resistant genetic source to this disease from C. annuum, we have decided to introduce the resistant gene(s) of C. baccatum into C. annuum through interspecific hybridization. However, we found out that some serious genetic barriers were existed between these two species. The first one was embryo abortion occurred during post-fertilization, resulting in no viable interspecific hybrid seeds obtained. So, we carried out embryo rescue with some success. We were able to get some true interspecific hybrid plants between these two species. However, there was second serious genetic barrier, which was turned out to be sterility of the interspecific F1 plants. We are trying to overcome this barrier by both backcrossing and chromosome doubling. In the same time, we are attempting to overcome this genetic barrier by using a bridge crossing. We found out that C. chinense and C. frutescens are the best candidate because these two species are fairly interspecifically compatible with both C. baccatum and C. annuum, respectively. We are able to get viable and partially fertile F1's, F2's and BC1 plants between C. baccatum with either C. chinense or C. frutescens. The resistant response in all these interspecific hybrids indicate that the resistance of C. baccatum is appeared to be controlled by a or more dominant allele(s).
박효근 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-
Plant transformation technique is thought to be powerful tools for plant breeding. Because it enables foreign genes from bacteria, viruses, and animals, which cannot be crossed with plants, to be inserted into the plant genome. We transferred herbicide resistance gene (bar) into the parental inbreds of commercial F1 hybrid variety of hot pepper, Kalag-kimjang No. 2, by Agrobacterium tumefaciens. Shoots from cotyledons were selected on medium containing 0.5 mg/L phosphinotricin. Transformants were confirmed by PCR analysis, Southern blot analysis, and bioassay. They showed tolerance to 0.2% Basta solution during bioassay. These transformants are expected to be applied to development of practical purity test of F1 hybrid variety.
Molecular Breeding for Plant Disease Resistance : Prospects and Problems
Park, Hyo-Guen The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.1
The technique of plant transformation has started to show off its great power in the area of plant breeding by commercially successful introduction of transgenic varieties such as herbicide tolerant soybean and insect resistant corn in USA with an unimaginable speed. However, in contrast with the great success in the commercialization of herbicide tolerance and insect resistance, the transformation works on disease resistance has not yet reached the stage of full commercialization. This review surveys the current status of molecular breeding for plant disease resistance and their limits and problems. Some novel ideas and approaches in molecular breeding for disease resistance are introduced.
Park, Hyo-Jin,Park, Soo-Yong,Kim, Jin-Woo,Yang, Seul-Gi,Kim, Min-Ji,Jegal, Ho-Guen,Kim, In-Su,Choo, Young-Kug,Koo, Deog-Bon MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.11
<P>Bisphenol A (BPA) is synthetic organic compound that exhibits estrogen-like properties and it induces mitochondrial superoxide production. Melatonin (Mela) protects against BPA-mediated cell damage and apoptosis. However, the antioxidative effects of Mela against BPA-induced superoxide production in porcine oocytes are still not known. In this study, we investigated the antioxidative effects of Mela against BPA-derived superoxide on oocyte maturation in pigs. To investigate the effects of the superoxide specific scavenger, Mito-TEMPO, on porcine oocyte maturation in response to BPA exposure apoptosis proteins, we treated the oocytes with Mito-TEMPO (0.1 µM) after pre-treating them with BPA (75 µM) for 22 h. As expected, the reduction in meiotic maturation and cumulus cell expansion of cumulus-oocyte-complexes (COCs) in the BPA (75 µM) treated group was recovered (<I>p</I> < 0.01) by treatment with Mito-TEMPO (0.1 µM). An increase in the levels of mitochondrial apoptotic proteins (AIF, cleaved Cas 3 and cleaved Parp1) in response to BPA-induced damage was also reduced by Mito-TEMPO treatment in porcine COCs. Interestingly, we confirmed the positive effects of Mela with respect to superoxide production upon BPA exposure during oocyte maturation and also confirmed the reduction in mitochondrial apoptosis in Mela (0.1 µM)-treated porcine COCs. These results provide evidence for the first time that antioxidative effects of Mela on BPA-derived superoxide improve porcine oocyte maturation.</P>
Hyo Guen Park,Inok Jang,Ji-Hye Moon,윤재복,Jae-Heung Yoo,Tae Jin Yang 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.3
A simpler and better method for purity testing of hybrid pepper seed was developed. The simplest method for extracting genomic DNA, the NaOH method, was chosen. Two RAPD markers identifying male and female parents were also developed, and the PCR products of male- and female-specific RAPD markers were cloned and sequenced. From these sequences, new longer primers were constructed for conversion into SCAR markers. In blind tests the RAPD and SCAR markers were able to reliably detect contaminating exotic seeds. These PCR-based markers are therefore directly applicable for purity testing by seed companies. In addition, the PCR products of the SCAR markers could be identified by direct staining methods such as ethidium bromide and pellet painting without electrophoresis.
Hyo-Jin Park,Jin-Woo Kim,Seul-Gi Yang1,Min-Ji Kim,Ho-Guen Jegal,Joung Jun Park,Deog-Bon Koo 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Bisphenol-A (BPA) as an organic synthetic compound of exhibiting estrogen-mimicking and hormone-like properties, which is commonly used to induce cellular stress or female reproductive toxicity. In addition, BPA induces the increasing of mitochondrial derived reactive oxygen species (ROS) such as superoxide, and production of these ROS affects to the meiotic maturation and cumulus cells expansion on in vitro maturation (IVM) of porcine cumulus-oocyte complexes (COCs). However, anti-oxidative effect of melatonin for reduction of BPA-induced superoxide on porcine oocyte maturation has not been reported. Therefore, in present study, we confirmed that the reduction of BPA-derived superoxide by melatonin related to the reducing of mitochondria mediated apoptosis on meiotic maturation and cumulus cells expansion of porcine COCs. Then, to investigate the effects of superoxide specific scavenger, Mito-TEMPO, during porcine oocyte maturation progression, COCs cultured in maturation medium with Mito-TEMPO (0.1 μM) after pre-treatment of BPA (75 μM) for 22 h. Reduced meiotic maturation rate and cumulus cells expansion of COCs in the BPA (75 μM) treated group were recovered (p<0.01) by Mito-TEMPO treatment. Also, increasing of mitochondria derived apoptotic factors (AIF, Cleaved Caspase 3 and Cleaved PARP 1) protein levels by BPA treatment were reduced by Mito-TEMPO treatment in porcine COCs maturation. Positive effects of Mito-TEMPO for superoxide reduction on oocyte maturation and reducing mitochondrial apoptosis showed the same pattern in melatonin (0.1 μM) treated COCs. In case of supplemented with BPA and melatonin, superoxide production in COCs was not changed compared to control or melatonin treated groups. Based on these results, we concluded that melatonin as a regulator of superoxide such as Mito-TEMPO improves oocyte maturation through reduction of mitochondria derived apoptosis during IVM of porcine COCs.