환경 오염물질의 진보된 독성 평가 기법

류재천,최윤정,김연정,김형태,방형애,송윤선 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.1

Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk^(+/-) gene assay (MOLY) using L5178Y tk+i- mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk^(+/-) gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells. Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D, C, in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.

도시지역 여자고등학생들의 체형과 체형에 대한 의식 및 자각증상과의 관계

류광하,권인선,김형선,조영채 한국학교보건학회 2008 韓國學校保健學會誌 Vol.21 No.2

Purpose: This study investigated the relationship between body type subjective symptoms and health awareness in female high school students Methods: The subjects were 393 students at the female high school in Taejon City by self-recorded questionnaires in May, 2006. Results: In the classification of body type based on the degree of obesity low weight group was 20.9% normal weight group was 58.0% over weight group was 11.7% and obesity group was 9.4% In the level of body type awareness those who consider themselves to be obesity type 13.7% standard type were 43.8% and leptosomic type 8.1% but there was a clear tendency to be leptosomic type that the rate was 76.3% In the complaint rates of subjective symptoms "fatigue" was the highest followed by "neck pain(stiffness)", "dizziness", "stomachache" in the descending order In the complaint rates of subjective symptoms by the degree of obesity dizziness irregular menstruation and anorexia were higher according to the lower obesity but constipation and wearied eyes were the higher the more obesity Conclusion: The study results showed that the recent female high school students were clear tendency to be leptosomic type and leptosomic and obesity type were related with subjective symptoms Considering these findings it appeared that being excessively thin is related to symptoms and young women's thinness-oriented attitudes are unhealthful.

초등학교 학생들의 공기에 대한 선개념 조사

조선형,유효진 청주교육대학교 과학교육연구소 2001 科學과 數學敎育論文集 Vol.22 No.-

The preconception is socalled the naive theories was made according to the general experience, direct investigation, wrong explanation on textbook or bibliography material and different of culture. By Champagne and klopfer(1983) find out that the preconception are latent in those blains long time, so they cause serious influences to the general teaching method without special healing. The researches on the air unit in primary school are first, the concept of air volume and its weight, secondly the difference of concepts between urban and local area, and third the distinction of sex. In this work, there are formed preconception in the third grade children primary school, urban children thought are more than local, and last male children have more right thought than female both urban and local area.

Acute oral toxicity of the ethyl acetate fraction of <i>Orostachys japonicus</i> in mice

Kim, Seon-Hee,Ryu, Deok-Seon,Lee, Hyeong-Seon,Shin, Hye-Ryoung,Kwon, Ji-Hye,Lee, Dong-Seok Informa Healthcare USA, Inc. 2014 PHARMACEUTICAL BIOLOGY Vol.52 No.10

<P><I>Context</I>: <I>Orostachys japonicus</I> (Crassulaceae) is referred to as Wa-song in Korea. It is used as an anti-inflammatory, antifebrile, hemostatic, and anti cancer agent, and as an antidote.</P><P><I>Objective</I>: The purpose of this study was to evaluate the acute toxicity of the ethyl acetate fraction of <I>O. japonicus</I> (OJE) after the oral administration in Balb/c mice of both sexes.</P><P><I>Materials and methods</I>: Mice were oral administered a single doses of 500, 1000, and 2000 mg/kg of body weight and were monitored for 14 d. Biochemical parameters [aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein (TP), globulin (GB), total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN), and creatinine (CR)] and histopathological examination of liver were performed.</P><P><I>Results and conclusion</I>: No animals died and no toxic changes were observed in clinical signs, body weight, and organ weight. The LD<SUB>50</SUB> of orally administered OJE was higher than 2000 mg/kg/d in both sexes. No toxicological findings were found in biochemical parameters. In histophathological examination, neutrophilic infiltration was observed at a dose of 2000 mg/kg group in both sexes. These finding suggest that oral administration of OJE does not produce acute toxicity. Therefore, these results could provide satisfactory preclinical evidence of safety to launch clinical trials on standardized formulation of OJE to be a biohealth product.</P>

A Repeated-dose Oral Toxicity Study of Orostachys japonicus Extract in Sprague-Dawley Rats

Deok-Seon Ryu,Mi-Young Lee,Hyeong-Seon Lee,Seon-Hee Kim,Gyeong-Seon Lee,Ji-Hye Kwon,Dong-Seok Lee 대한의생명과학회 2012 Biomedical Science Letters Vol.18 No.1

A 12-week repeated-dose oral toxicity study of water-soluble Orostachys japonicus extract (WOJ) was performed in Sprague-Dawley (SD) rats of both genders. Each group of ten rats was orally administered in doses of either 0 or 250 mg/day over a 12-week period. As a result, no WOJ-related changes were observed in terms of survival rate, clinical signs, body weight, or food intake. In addition, no difference in organ weight between the control and treated groups was detected. Furthermore, serum biochemistry parameters revealed some changes within normal ranges although significant decreases in total-bilirubin in the females. In spite of some alterations in serum biochemistry, the clinical signs, body weight changes from food intake, and autoptical remarks indicated that WOJ was not toxic. This study suggests that repeated treatment of O. japonicus very low toxicity and the NOAEL (no observed adverse effect dose) of WOJ exceeds 250 mg/kg in the SD rats.

연평도 일대의 식물상 연구

유혜선 ( Hye Seon Ryu ),박수현 ( Su Hyun Park ),장계선 ( Kae Sun Chang ),최형선 ( Hyeong Seon Choi ),하상교 ( Sang Gyo Ha ),이혜정 ( Hye Jeong Lee ),이유미 ( You Mi Lee ) 한국환경생태학회 2013 한국환경생태학회지 Vol.27 No.2

인천광역시 옹진군에 속한 서해 5도 중 한 곳인 연평도일대에 자생하는 관속식물들을 밝히고 주요 식물들을 파악하였다. 2010년 5월부터 2010년 10월까지 총 4차례 현지조사를 통해 밝혀진 소산식물은 90과 315속 459종 4아종 53변종 8품종의 524분류군으로 조사되었다. 이 중 한반도 고유종은 총 2분류군, 산림청 지정 한국 희귀식물은 8분류군이 조사되었다. 구계학적 특정식물은 Ⅴ등급이 2분류군, Ⅳ등급이 2분류군, Ⅲ등급이 4분류군, Ⅱ등급이 3분류군, Ⅰ등급에 27분류군 등 총 38분류군이 분포하고 있는 것을 알 수 있었다. 귀화식물은 총 66분류군으로 조사되었으며, 귀화율은 12.6%로 높게 나타났다. The vascular plants of Is. Yeonpyeongdo regions, located in Ongjin-gun, Incheon were surveyed from May to October 2010. The vascular plants collected 4 times were consisted of total 524 taxa; 90 families, 315 genera, 459 species, 4 subspecies, 53 varieties and 8 formas respectively. Among them, 2 taxa endemic species of Korea and 8 taxa of Korean rare plants were investigated in addition to 38 taxa of specially designated plants announced by the Ministry of Environment. The naturalized plants were 66 taxa, therefore naturalized ratio was12.6% highly.

대학 내 학문 목적 한국어 학습자를 위한 교양한국어 교재 개발 기초 연구

류선숙 ( Ryu Seon-suk ),김민경 ( Kim Min-kyung ),전형길 ( Jeon Hyeong-gil ) 한국어문학국제학술포럼 2021 Journal of Korean Culture Vol.55 No.-

본 연구의 목적은 국내 대학에 재학 중인 외국인 유학생들의 학문적 한국어 숙달도 신장을 위한 교재 개발 방향을 모색하는 데 있다. 이를 위하여 국내 주요 대학의 한국어 교과목 개설 및 교재 개발 현황을 파악하고 서울 소재 A대학의 외국인 학부생 345명을 대상으로 교양한국어 교재 개발에 대한 요구 조사를 실시하였다. 조사 결과, 숙달도를 고려한 등급별 교재 개발과 기능 수행 및 학문적 과제 활동을 위한 언어지식 강화형 교재에 대한 요구가 높은 것을 확인하였다. 이에 본고에서는 등급별 교양한국어 교재 개발과 대학 생활 적응 및 학업 수행 지원, 대학 교과 내용 및 기초지식 함양이라는 교재 개발의 방향성을 제시하였으며 학문 맥락의 실제적인 의사소통 과제 속에서 자연스럽게 언어 지식을 익히도록 하는 ‘의사소통 과제 중심의 언어지식 강화형’ 교재 모형을 도출하였다. This study aimed to explore the direction of development of textbooks to improve Korean language competency of foreign students studying in Korean universities. For this, the author explored and identified opening of Korean language subjects in major Korean universities and the status of textbook development. The author conducted a demand survey on the development of textbooks of Korean language as liberal arts for 345 foreign undergraduates. Based on the results, the author could confirm the necessity for development of a textbook for step-by-step and concentrative Korean language education conducted within universities currently. It was found that the demand for development of textbooks by grades in consideration of differences of competency, functioning and linguistic knowledge-strengthening textbooks was high among undergraduates. Therefore, this paper suggested a direction for development of textbooks for Korean language as liberal arts, adaptation to university lives and support for studying as well as textbook contents and basic knowledge enhancement. It derived ‘communication-based linguistic knowledge strengthening’ that would enable the students to naturally acquire linguistic knowledge in actual communication tasks in the context of learning.

도시철도 전력설비 이상검출용 장치 공장시험에 대한 고찰

임형길(Hyeong-Gil Im),유기선(Ki-Seon Ryu),정호성(Ho-Sung Jung),최광범(Kwang-Bum Choi),이기승(Gi-Seung Lee) 한국철도학회 2010 한국철도학회 학술발표대회논문집 Vol.2010 No.7

Because of the environmental matters the importance of the city railroad is as time goes by increasing. The case of obstacle of the power equipment which supplies electric power to city railroad will occur social and economical enormous loss. Thus, I studied on the preventing method in advance which makes it possible for us to maintain facilities efficiently. The main check points of the power facilities are voltage, current, humidity, partial discharge, move current. These points are gathered by sensor and transmitting to data acquisition device. These data are used to check equipment status in real time. In this paper I described in brief test process and results of the detection system.

SF₆의 플라즈마 분해로부터 생성된 입자상 물질에 관한 연구

김선우(Seon-Woo Kim),김종범(Jong-Bum Kim),김재환(Jae-Hwan Kim),김래형(Rae-Hyeong Kim),류재용(Jae-Yong Ryu) 한국대기환경학회 2017 한국대기환경학회지 Vol.33 No.4

도시철도용 몰드변압기 부분방전 신호특성 분석

임형길(Hyeong-Gil Im),유기선(Ki-Seon Ryu),최광범(Kwang-Bum Choi),이기승(Gi-Seung Lee) 한국철도학회 2010 한국철도학회 학술발표대회논문집 Vol.2010 No.10