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        Novel Method for Calculating the Effective Stress Coefficient in a Tight Sandstone Reservoir

        Yinghao Shen,Guohua Luan,Haiyong Zhang,Qian Liu,Junjing Zhang,Hongkui Ge 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.6

        Determining the influence of effective stress on rock deformation is essential for geotechnical stability analysis in oil and gas production. There is no universal effective stress coefficient for all rock properties, and different values of effective stress coefficient apply for different physical quantities (Gurevich, 2004). Although the effective stress law and its application is not new, frequently overlooked or misapplied. Hence, a novel method was proposed for measuring and calculating the effective stress coefficient in this work. Firstly, pore compressibility under different confine pressure values was measured using reservoir fluid or experimental fluid. Secondly, effective stress was calculated by comparing pore compressibility under different confine pressure and then, the range of effective stress coefficients was determined eventually. Finally, the reliability of the proposed method was validated via the stresssensitive curves of tight sandstone core samples and by comparing the results with those of two other calculation methods for the effective stress coefficient. This work suggests that the stress-sensitive curves of the two core samples from the same location and with similar physical properties have given similar effective stress coefficient (η = 0.201) calculated using the proposed method, which indicates that the calculation is reasonable. The comparison of the proposed method with other methods also indicate that the proposed technique is reliable.

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        Analysis of the DNA Methylation of Maize (Zea mays L.) in Response to Cold Stress Based on Methylation-sensitive Amplified Polymorphisms

        Xiaohui Shan,Xiaoyu Wang,Guang Yang,Ying-Wu Lan,Shengzhong Su,Shipeng Li,Hongkui Liu,Yaping Yuan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1

        DNA methylation plays a vital role in tuning geneexpression in response to environmental stimuli. Here,methylation-sensitive amplified polymorphisms (MSAP)were used to assess the effect of cold stress on the extent andpatterns of DNA methylation in maize seedlings. Overall,cold-induced genome-wide DNA methylation polymorphismsaccounted for 32.6 to 34.8% of the total bands at the differenttreatment time-points. It was demonstrated that the extentand pattern of DNA methylation was induced by cold stressthrough the cold treatment process and that thedemethylation of fully methylated fragments was the maincontributor of the DNA methylation alterations. The sequencesof 28 differentially amplified fragments relevant to stresswere successfully obtained. Under the cold stress, demethylationwas detected in most fragments. BLAST results indicate thatthe homologues of these fragments are involved in manyprocesses, including hormone regulation, cold response,photosynthesis, and transposon activation. The expressionanalysis demonstrated an increase in the transcription of fivedemethylated genes. Despite the fact that DNA methylationchanges and cold acclimation are not directly associated, ourresults may indicate that the specific demethylation of genesis an active and rapid epigenetic response to cold in maizeduring the seedling stage, further elucidating the mechanismof maize adaptation to cold stress.

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