http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ishibashi, Hiroshi,Hirano, Masashi,Kim, Eun-Young,Iwata, Hisato American Chemical Society 2019 Environmental science & technology Vol.53 No.4
<P>In this study, we assessed the binding affinities of perfluoroalkyl substances (PFASs), including perfluoroalkyl carboxylates (PFCAs) and perfluoroalkyl sulfonates (PFSAs), to the ligand-binding domains (LBDs) of Baikal seal (<I>Pusa sibirica</I>; bs) and human (h) peroxisome proliferator-activated receptor alpha (PPARα). An in vitro competitive binding assay showed that six PFCAs and two PFSAs could bind to recombinant bs and hPPARα LBD proteins in a dose-dependent manner. The relative binding affinities (RBAs) of PFASs to bsPPARα were as follows: PFOS > PFDA > PFNA > PFUnDA > PFOA > PFHxS > PFHpA > PFHxA. The RBAs to bsPPARα showed a significant positive correlation with those to hPPARα. In silico PPARα homology modeling predicted that there were two ligand-binding pockets (LBPs) in the bsPPARα and hPPARα LBDs. Structure-activity relationship analyses suggested that the binding potencies of PFASs to PPARα might depend on LBP binding cavity volume, hydrogen bond interactions, the number of perfluorinated carbons, and the hydrophobicity of PFASs. Interspecies comparison of the in vitro binding affinities revealed that bsPPARα had higher preference for PFASs with long carbon chains than hPPARα. The in silico docking simulations suggested that the first LBP of bsPPARα had higher affinities than that of hPPARα; however, the second LBP of bsPPARα had lower affinities than that of hPPARα. To our knowledge, this is the first evidence showing interspecies differences in the binding of PFASs to PPARαs and their structure-activity relationships.</P> [FIG OMISSION]</BR>
CONSTRAINTS ON A-DECAYING COSMOLOGY FROM OBSERVATIONAL POINT OF VIEW
KOMIYA ZEN,KAWABATA KIYOSHI,HIRANO KOICHI,BUNYA HIROSHI,YAMAMOTO NAOTAKA The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.2
To constrain the values of the model parameters for the cosmological models involving the time-decaying $\Lambda$ term, we have computed sets of theoretical predictions for the N-m relation of galaxies as well as the CMB angular power spectrum: three types of variation, viz., ${\Lambda}{\propto} T^{-1},\;a^{-m}$, and $H^n$ are thereby assumed following Overduin & Cooperstock (1998), although we concentrate here on the discussion of the results obtained from the first type. Our results for the N-m relation indicate that the observed excess of the galaxy counts N in the faint region beyond the blue apparent magnitude 24 can be reasonably well accounted for with the value of ${\iota}$ in the range between 0.2 and 1. Furthermore, a comparison of our computational results of the CMB spectra with the observational data shows that the models with a mild degree of the $\Lambda$ term decay, viz., with the value of ${\iota}{\le}$0.4, are favorable. In this case, the age of our universe turns out to be larger than or equal to 14 Gyr, the lower limit inferred from some Uranium datings.
Dau, Pham Thi,Sakai, Hiroki,Hirano, Masashi,Ishibashi, Hiroshi,Tanaka, Yuki,Kameda, Kenji,Fujino, Takahiro,Kim, Eun-Young,Iwata, Hisato Academic Press 2013 Toxicological sciences Vol.131 No.1
<P>The constitutive androstane receptor (CAR) not only displays a high basal transcriptional activity but also acts as a ligand-dependent transcriptional factor. It is known that CAR exhibits different ligand profiles across species. However, the mechanisms underlying CAR activation by chemicals and the species-specific responses are not fully understood. The objectives of this study are to establish a high-throughput tool to screen CAR ligands and to clarify how CAR proteins from the Baikal seal (bsCAR) and the mouse (mCAR) interact with chemicals and steroid receptor coactivator 1 (SRC1). We developed the surface plasmon resonance (SPR) system to assess quantitatively the interaction of CAR with potential ligands and SRC1. The ligand-binding domain (LBD) of bsCAR and mCAR was synthesized in a wheat germ cell-free system. The purified CAR LBD was then immobilized on the sensor chip for the SPR assay, and the kinetics of direct interaction of CARs with ligand candidates was measured. Androstanol and androstenol, estrone, 17β-estradiol, TCPOBOP, and CITCO showed compound-specific but similar affinities for both CARs. The CAR-SRC1 interaction was ligand dependent but exhibited a different ligand profile between the seal and the mouse. The results of SRC1 interaction assay accounted for those of our previous in vitro CAR-mediated transactivation assay. In silico analyses also supported the results of CAR-SRC1 interaction; there is little structural difference in the ligand-binding pocket of bsCAR and mCAR, but there is a distinct discrimination in the helix 11 and 12 of these receptors, suggesting that the interaction of ligand-bound CAR and SRC1 is critical for determining species-specific and ligand-dependent transactivation over the basal activity. The SPR assays demonstrated a potential as a high-throughput screening tool of CAR ligands.</P>
Rino Hasegawa,Kenshi Yao,Shoutomi Ihara,Masaki Miyaoka,Takao Kanemitsu,Kenta Chuman,Go Ikezono,Akikazu Hirano,Toshiharu Ueki,Hiroshi Tanabe,Atsuko Ota,Seiji Haraoka,Akinori Iwashita 대한소화기내시경학회 2018 Clinical Endoscopy Vol.51 No.6
Background/Aims: While the occurrence of multiple whitish flat elevated lesions (MWFL) was first reported in 2007, no studieson MWFL have been published to date. The present retrospective observational study aimed to clarify the endoscopic findings andclinicopathological features of MWFL. Methods: Subjects were consecutive patients who underwent upper gastrointestinal endoscopy as part of routine screening betweenApril 2014 and March 2015. The conventional white-light, non-magnifying and magnifying narrow-band images were reviewed. Clinical features were compared between patients with and without MWFL. Results: The conventional endoscopic findings of MWFL include multiple whitish, flat, and slightly elevated lesions of various sizes,mainly located in the gastric body and fundus. Narrow-band imaging enhanced the contrast of MWFL and background mucosa,and magnifying narrow-band imaging depicted a uniformly long, narrow, and elliptical marginal crypt epithelium with an unclearmicrovascular pattern. Histopathological findings revealed hyperplastic changes of the foveolar epithelium, and parietal cell protrusionsand oxyntic gland dilatations were observed in the fundic glands, without any intestinal metaplasia. The rate of acid-reducing drug usewas significantly higher in patients with MWFL than in those without (100% [13/13] vs. 53.7% [88/164], p<0.001). Conclusions: The present study indicated a relationship between the presence and endoscopic features of MWFL and history of acidreducingdrug use.