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        Chrysanthemum extract and extract prepared silver nanoparticles as biocides to control Aedes aegypti (L.), the vector of dengue fever

        Hamed A. Ghramh,Kh.M. Al-Ghamdi,Jazem A. Mahyoub,Essam H. Ibrahim 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

        Mosquitoes play a key role in the transmission of some important diseases. The need for controlling these insectsis critical to reduce their risks to human and domesticated animals. Recently the trend to explore effectivechemical compounds from local plants has begun as a safe means of control. The present study aimed to evaluatethe anti-larval activity of Chrysanthemum extract and the prepared silver nanoparticle (AgNPs) against the Aedesaegypti mosquito, the dengue vector in Saudi Arabia. A series of different concentrations of ethanol extract andextract prepared AgNPs against the fourth-life larvae was tested. The effective concentrations of crude extractand AgNPs ranged from 50 to 250 and 10 to 30 ppm respectively, and the death percentages corresponding tothese concentrations ranged from 18 to 92 and 36 to 96% respectively. According to the LC50 values of treatedlarvae, AgNPs (12.754 ppm) is more effective against A. aegypti mosquito larvae than the crude extract(228.345 ppm) at about 17.9 times. The mixing of the plant extract with the silver nitrate has led to potentiation. This is due to the synergy that occurs between the extract and the silver particles during the reduction process. The compounds in the extract are related to the surface of the particles, increasing the strength of their effects. Itis recommend to separate the active elements in the Chrysanthemum plant and its preparation in the form ofnanoparticles as a promising compound in mosquito control programs with least damage to human kind and theenvironment.

      • KCI등재

        Diversity of pollinators and their role in the pollination biology of chickpea, Cicer arietinum L. (Fabaceae)

        Abdul Latif,Saeed Ahmad Malik,Shafqat Saeed,Naeem Iqbal,Qamar Saeed,Khalid Ali Khan,Chen Ting,Hamed A. Ghramh 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

        Chickpea (Cicer arietinum L.) is an important source of food for people worldwide. In the current study, we studied its pollination biology with special reference to floral visitors along with their visitation rate, frequency and pollen load during 2012 and 2013. We also explored the effect of floral visitors on the capsule weight, seed weight, seed numbers and seed germination. Results revealed three bees, two wasps, five flies, one moth and three butterfly species on the flowers of chickpea. Apis dorsata, A. florea, Amegilla sp. and Eristalinus aeneus were the major species with 434–474, 223–311, 69–74 and 81–136 individuals, respectively in both years. Floral visitors differed significantly in term of visitation frequency with A. florea as the most frequent visitor (9.13–9.86 visits/flower/5 min) followed by E. aeneus (5.43–5.58 visits/flower/5 min) and A. dorsata (1.72–2.31 visits/ flower/5 min) in both years. Similarly, A. florea had statistically highest visitation rate (16.85–19.99 flowers visited/min) followed by E. aeneus (9.73–10.68 flowers visited/min). A. dorsata had significantly higher pollen load on its body (84629–85,104 pollen grains) followed by A. florea (64940–65,135 pollen grains) and Amegilla sp. (64020–65,120 pollen grains). The open-pollinated flowers had significantly higher capsule weight (0.27 ± 0.01 g), seed weight (0.18 ± 0.01 g), seed numbers (1.67 ± 0.07 seeds) and seed germination (95 ± 1.38%) as compared to flowers deprived of pollinators in cages. The results suggested A. florea, A. dorsata and E. aeneus could be effective pollinators of chickpea. Hence these three species can be properly utilized on commercial scale to increase crop yield.

      • KCI등재

        In-vitro assessment of food consumption, utilization indices and losses promises of leafworm, Spodoptera litura (Fab.), on okra crop

        Ahmad Nawaz,Habib Ali,Muhammad Sufyan,Muhammad Dildar Gogi,Muhammad Jalal Arif,Abid Ali,Muhammad Qasim,Waqar Islam,Noman Ali,Imran Bodla,Madiha Zaynab,Khalid Ali Khan,Hamed A. Ghramh 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

        The lepidopteran insect pests have significant importance in vegetable production. The present study was performed to investigate the baseline studies about the assessment of feeding and consumption potential, utilization indices and losses promises of leafworm, Spodoptera litura (Fab.) on Okra. The data regarding feeding potential, food utilization and consumption indices as well as losses of different larval instars were recorded and subjected to appropriate statistical analysis. The results showed that, in the beginning, the approximate digestibility of various instars was increase, e.g. third instar (51.36%–64.03%), fourth instar (63.42%–69.45%) and fifth instar (70.25%–76.10%). However, after a certain period, the digestibility was decreased and efficiency to convert the ingested food into biomass varied significantly. The consumption index values increased with an increase in time but the consumption and growth rate was declined of fourth instar larvae. The ingestion and digestion increased of third (10.01–13.06, 8.32–11.91 mg), fourth (11.27–17.28, 10.96–14.03 mg) and fifth (12.60–19.40, 11.93–15.28 mg) larval instars. The corrected weight of consumed leaves increased with a gain in body weight. However, in the third instar, a decline was observed on the last day of feeding. Maximum leaf area was consumed by fifth instar larvae (44.66 cm 2 ) followed by fourth (35.41 cm 2 ) and third (27.98 cm 2 ) instars. In conclusion, all the dependent parameters, including food utilization potential, consumption indices and losses were higher for fifth instar larvae than others. These results emphasized the re-establishment of fundamental (economic threshold level: ETL, economic injury level: EIL) integrated pest management concepts.

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