Impact of Latrogenic Biliary Injury during Laparoscopic Cholecystectomy on Surgeon’s Mental Distress A Nationwide Survey from China

( Tian Yang ),( Hai-su Dai ),( Lei Liang ),( Cheng-cheng Zhang ),( Zhang-jun Cheng ),( Yong-hai Peng ),( Yao-ming Zhang ),( Zhi-yu Chen ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Iatrogenic biliary injury (IBI) following laparoscopic cholecystectomy (LC) is the most common and recognized iatrogenic complications. Little is known whether LC-IBI would lead to surgeon’s mental distress. This study reports the incidence of surgeon’s mental distress who have caused LC-IBI and risk factors of surgeon’s severe mental distress (SMD). Methods: A cross-sectional survey in the form of electronic questionnaire was conducted among Chinese general surgeons who have caused LC-IBI. The six collected clinical features relating to mental distress included: 1) feeling burnout, anxiety, or depression, 2) avoiding performing LC, 3) having physical reactions when recalling the incidence, 4) having the urge to quit surgery, 5) taking psychiatric medications, and 6) seeking professional psychological counseling. Univariable and multivariable analyses were performed to identify risk factors of SMD, which was defined as meeting ≥3 of the above-mentioned clinical features. Results: Among 1,466 surveyed surgeons, 1,236(84.3%) experienced mental distress following LC-IBI, and nearly half (49.7%, 614/1236) had SMD. Multivariable analyses demonstrated that surgeons from non-university affiliated hospitals (OR:1.873), patients who required multiple repair operations (OR:4.075), patients who required hepaticojejunostomy/partial hepatectomy (OR:1.859), existing lawsuit litigation (OR:10.491), existing violent doctor-patient conflicts (OR:4.995), needing surgeons’ personal compensation (OR:2.531), and additional administrative punishment by hospitals (OR:2.324) were independent risk factors of surgeon’s SMD. Conclusions: Four out of five surgeons experienced mental distress following LC-IBI, and nearly half had SMD. Several independent risk factors of SMD were identified, which could help to make strategies to improve mental well-being of these surgeons.

Intervention Effects of Nedaplatin and Cisplatin on Proliferation and Apoptosis of Human Tumour Cells in Vitro

Su, Xiang-Yu,Yin, Hai-Tao,Li, Su-Yi,Huang, Xin-En,Tan, Hua-Yang,Dai, Hong-Yu,Shi, Fang-Fang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To study synergistic effects of nedaplatin and cisplatin on three human carcinoma cell lines (esophageal carcinoma cell line Eca-109, ovarian carcinoma Skov-3 and cervical carcinoma Hela). Methods: Inhibition effects were evaluated by MTT assay and cell apoptosis was detected by flow cytometry. In addition, changes of Ki-67, Bax and Bcl-2 at mRNA and protein levels were quantified by RT-PCR and Western blotting. Results: Growth inhibition in each cell lines was dose-dependent after exposure to nedaplatin or cisplatin alone. The interaction of the two drugs was synergistic at higher concentrations according to the median-effect principle. The inhibition rates with nedaplatin, cisplatin and combined treatment were $41.9{\pm}4.1%$, $47.4{\pm}2.9%$, $52.5{\pm}0.9%$(Eca-109), $39.0{\pm}1.26%$, $45.0{\pm}1.45%$, $56.2{\pm}1.44%$ (Skov-3) and $44.8{\pm}2.11%$, $46.9{\pm}0.99%$, $56.6{\pm}1.83%$ (Hela) respectively, with increase in apoptosis. Compared with the nedaplatin or cisplatin alone treatment group, the combinative treatment group's Ki-67 and bcl-2 mRNA (protein) expression was decreased while that of Bax mRNA (protein) was increased. Conclusion: Compared to the effects of nedaplatin or cisplatin alone at high concentrations, combination of nedaplatin and cisplatin at low concentrations proved to be much more effective for inhibition of proliferation and the induction of apoptosis in the Eca-109, Skov-3 and Hela cell lines.

Design and transmittance measurement of the Ge1-xCx antireflection coatings

Chang Yong Zhan,Li Wu Wang,Hai Yang Dai,N. K. Huang 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.-

Ge1-xCx is used as high antireflection and protection coating on ZnS and ZnSe. Ge1-xCx coatings with a type of design of gradient index layers can be used in very broad bands. A technique combining medium frequency (MF) with direct-current (DC) magnetron reactive sputtering was used to deposit Ge1-xCx films based on our theoretical designs. The results according to experimental measurements show that the highest transmittance of Ge1-xCx coated on ZnSe slab can reach 94% at 8.5 μm, to transmittance value above 80\% can be obtained within the whole region of 5.8-12 μm and an average transmittance of 82.4% can be obtained within the range of 2.3 -- 14 μm. The average transmittances of ZnSe slab deposited with Ge1-xCx are 20 -- 30% higher than that of ZnSe in the corresponding band area. Ge1-xCx is used as high antireflection and protection coating on ZnS and ZnSe. Ge1-xCx coatings with a type of design of gradient index layers can be used in very broad bands. A technique combining medium frequency (MF) with direct-current (DC) magnetron reactive sputtering was used to deposit Ge1-xCx films based on our theoretical designs. The results according to experimental measurements show that the highest transmittance of Ge1-xCx coated on ZnSe slab can reach 94% at 8.5 μm, to transmittance value above 80\% can be obtained within the whole region of 5.8-12 μm and an average transmittance of 82.4% can be obtained within the range of 2.3 -- 14 μm. The average transmittances of ZnSe slab deposited with Ge1-xCx are 20 -- 30% higher than that of ZnSe in the corresponding band area.

Stereo-digital image correlation in the behavior investigation of CFRP-steel composite members

Xiao-Yuan He,Yun-Tong Dai,Hai-Tao Wang,Tian-Yuan Ge,Gang Wu,Jian-Xiao Wan,Shuang-Yin Cao,Fu-Jun Yang 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.23 No.6

The application of carbon fiber reinforced polymer (CFRP) in steel structures primarily includes two categories, i.e., the bond-critical application and the contact-critical application. Debonding failure and buckling failure are the main failure modes for these two applications. Conventional electrometric techniques may not provide precise results because of the limitations associated with single-point contact measurements. A nondestructive full-field measurement technique is a valuable alternative to conventional methods. In this study, the digital image correlation (DIC) technique was adopted to investigate the bond behavior and buckling behavior of CFRP-steel composite members. The CFRP-to-steel bonded joint and the CFRPstrengthened square hollow section (SHS) steel column were tested to verify the suitability of the DIC technique. The stereo- DIC technique was utilized to measure continuous deformation. The bond-slip relationship of the CFRP-to-steel interface was derived using the DIC data. Additionally, a multi-camera DIC system consisting of four stereo-DIC subsystems was proposed and applied to the compressive test of CFRP-strengthened SHS steel column. The precise buckling location and CFRP delamination of the CFRP-strengthened SHS steel column were identified. The experimental results confirm that the stereo-DIC technique can provide effective measurements for investigating the behaviors of CFRP-steel composite members.

Transcriptome analysis of flower colour reveals the correlation between SNP and differential expression genes in Phalaenopsis

Ding Yu,Wang Ma-Yin,Yang Ding-Hai,Hao Dai-Cheng,Li Wei-Shi,Ling Peng,Xie Shang-Qian 한국유전학회 2023 Genes & Genomics Vol.45 No.12

Background Phalaenopsis is an important ornamental plant that has great economic value in the world flower market as one of the most popular flower resources. Objective To investigate the flower colour formation of Phalaenopsis at the transcription level, the genes involved in flower color formation were identified from RNA-seq in this study. Methods In this study, white and purple petals of Phalaenopsis were collected and analyzed to obtained (1) differential expression genes (DEGs) between white and purple flower color and (2) the association between single nucleotide polymorphisms (SNP) mutations and DEGs at the transcriptome level. Results The results indicated that a total of 1,175 DEGs were identified, and 718 and 457 of them were up- and down-regulated genes, respectively. Gene Ontology and pathway enrichment showed that the biosynthesis of the secondary metabolites pathway was key to color formation, and the expression of 12 crucial genes (C4H, CCoAOMT, F3’H, UA3’5’GT, PAL, 4CL, CCR, CAD, CALDH, bglx, SGTase, and E1.11.17) that are involved in the regulation of flower color in Phalaenopsis. Conclusion This study reported the association between the SNP mutations and DEGs for color formation at RNA level, and provides a new insight to further investigate the gene expression and its relationship with genetic variants from RNA-seq data in other species.

OrMKK3 Influences Morphology and Grain Size in Rice

Ying Hua Pan,Li Jun Gao,Yun Tao Liang,Yan Zhao,Hai Fu Liang,Wei Wei Chen,Xing Hai Yang,Dong Jin Qing,Ju Gao,Hao Wu,Juan Huang,Wei Yong Zhou,Cheng Cui Huang,Gao Xing Dai,Guo Fu Deng 한국식물학회 2023 Journal of Plant Biology Vol.66 No.3

Although morphology and grain size are important to rice growth and yield, the identity of abundant natural allelic variations that determine agronomically important differences in crops is unknown. Here, we characterized the function of mitogen-activated protein kinase 3 from Oryza officinalis Wall. ex Watt encoded by OrMKK3. Different alternative splicing variants occurred in OrMKK3. Green fluorescent protein (GFP)–OrMKK3 fusion proteins localized to the cell membrane and nuclei of rice protoplasts. Overexpression of OrMKK3 influenced the expression levels of the grain size-related genes SMG1, GW8, GL3, GW2, and DEP3. Phylogenetic analysis showed that OrMKK3 is well conserved in plants while showing large amounts of variation between indica, japonica, and wild rice. In addition, OrMKK3 slightly influenced brassinosteroid (BR) responses and the expression levels of BR-related genes. Our findings thus identify a new gene, OrMKK3, influencing morphology and grain size and that represents a possible link between mitogen-activated protein kinase and BR response pathways in grain growth.