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임윤규(Y.K. Lim),K. Okazaki,S. Matsuki,K. Ogasawara,H. Kida,이영순(Y.S. Lee) 한국예방수의학회 1998 예방수의학회지 Vol.22 No.3
Synthetic petide vaccines prepared according to the cassette theory (Naruse et al., 1994), formalin-inactivated virus and ether-split vaccine of influenza virus A/Aichi/68 (H3N2) were administered intranasally to the pigs. The peptide vaccines contained single or double sets of the peptide sequence of hemagglutinin 127~133 of influenza virus A/Aichi/2/68(H3N2) inserted into the H-2 class allele specific amino acid motif of the agretope composed of residues 43-58 of pigeon cytochrome c in the mouse bearing Ak or Ab. After 4 times immunization, pigs were challenged with 10⁴⁻⁵ PFU of virus intranasally. Intranasal immunization of the pigs with inactivated virus vaccine and split virus vaccine induced antibody production against A/Aichi/2/68(H3N2) virus, protecting them from infection. Synthetic peptide vaccine which contains 2 sets of epitope amino acid sequence evoked protective immune response although antibody response was not detected in the pigs. The present results indicated that intranasal route can be applied for inducing not only mucosal immunity but also systemic immunity against influenza virus infection. Based on the present finding we assume that synthetic peptide vaccine prepared according to Cassette theory may be applicable to the heterogenous species including human.
Lim, Y.K.,Okazaki, K.,Matsuki, S.,Ogasawara, K.,Kida, H.,Lee, Y. S. 濟州大學校 農科大學 動物科學硏究所 1998 動物科學論叢 Vol.13 No.1
Synthetic petide vaccines prepared according to the cassette theory(Naruse et al., 1994), formalin-inactivated virus and ether-split vaccine of influenza virus A/Aichi/68(H3N2) were administered intranasally to the pigs. The peptide vac-cines contained single or double sets of the peptide sequence of hemagglutinin 127-133 of influenza virus A/Aichi/2/68(H3N2) inserted into the H-2 class allele specific amino acid motif of the agretope composed of residues 43-58 of pigeon cytochrome c in the mouse bearing A^(k) or A^(b). After 4 times immuniza- tion, pigs were challenged with 10^(4-5) PFU of virus intranasally. Intranasal immunization of the pigs with inactivated virus vaccine and split virus vaccine induced antibody production against A/Aichi/2/68( H3N2) virus, protecting them from infection. Synthetic peptide vaccine which contains 2 sets of epitope amino acid sequence evoked protective immune response although antibody response was not detected in the pigs. The present results indicated that intranasal route can be applied for inducing not only mucosal immunity but also systemic immunity against influenza virus infection. Based on the present finding we assume that synthetic peptide vaccine prepared according to Cas-sette theory may be applicable to the heterogenous species including human.
M. Tanamura,Yukio Watanabe,H. Kida,Naotake Toyama 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.IV
A new approach of the ion milling is proposed, utilizing the mass selectivity of the sputtering process. As model experiments, we performed etching of conducting perovskite oxide lms that are often used as electrode of ferroelectric lms and are known to be extremely structure-sensitive. By using He, N2, Ar and Kr as an etching gas, various etching characteristics are found to depend evidently on the molecular weight of the gas. Substantial improvements are demonstrated by using Kr that has the heaviest molecular weight. To explain the results, a semi-empirical extension of Sigmund theory is proposed. The present approach provides a new way to etch metal oxides including ferroelectrics by utilizing the atomic/molecular mass eect in the sputtering.
Identification of the DSPP mutation in a new kindred and phenotype–genotype correlation
Lee, S‐,K,Lee, K‐,E,Hwang, Y‐,H,Kida, M,Tsutsumi, T,Ariga, T,Park, J‐,C,Kim, J‐,W Blackwell Publishing Ltd 2011 Oral diseases Vol.17 No.3
<P> <I>Oral Diseases</I> (2011) <B>17</B>, 314–319</P><P><B>Objective: </B> Hereditary dentin defects can be grouped into three types of dentinogenesis imperfecta (DGI) and two types of dentin dysplasia. Tooth enamel is considered normal in patients with hereditary dentin defects, but is easily worn down and fractured due to <I>DSPP</I> mutation‐induced altered dentin properties. The purposes of this study were to identify genetic cause of a family with type II DGI and enamel defects.</P><P><B>Materials and methods: </B> We identified a family with type II DGI and a unique form of hypoplastic enamel defect affecting occlusal third of the crown. Family members were recruited for the genetic analysis and DNA was obtained from peripheral whole blood.</P><P><B>Results: </B> Mutational analysis revealed a T to A transversion in exon 3 of the <I>DSPP</I> (c.53T>A, p.V18D). Haplotype analysis showed that the same mutation arose separately in two different families having DGI with similar enamel defects, indicating that this phenotype is associated with this specific <I>DSPP</I> mutation. Clinical features suggest that enamel formation was affected in the affected individuals during early amelogenesis, in addition to the dentin defect.</P><P><B>Conclusions: </B> We observed that a <I>DSPP</I> gene mutation not only influences dentinogenesis but also affects early stage amelogenesis.</P>
Pen, B.,Iwama, T.,Ooi, M.,Saitoh, T.,Kida, K.,Iketaki, T.,Takahashi, J.,Hidari, H. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.9
The effect of substituting potato by-products based silage (PBS) for concentrates on ruminal fermentation, methane production and nitrogen utilization in Holstein steers was evaluated. Three growing Holstein steers ($490{\pm}19kg$, initial body weight) were used in a $3{\times}3$ Latin square experiment with three experimental diets in which PBS was included at (1) 0, (2) 19 and (3) 27%, on a dry matter basis, replacing concentrates and wheat bran. Increasing replacement levels of PBS slightly increased dry matter (DM), organic matter (OM), NDF and ADF intakes by the steers (p<0.05). Inclusion of PBS at 19% increased crude protein (CP) digestibility of the experimental diets compared with the control (p<0.05). Substitution with PBS increased ADF digestibility and nitrogen retention (p<0.05), but did not affect energy retention. Energy loss as methane ranged between 5.0 and 6.1% of the total gross energy intake. There were no significant differences in carbon dioxide and methane production among all PBS levels, while daily methane production numerically increased with PBS inclusion. Substituting PBS for concentrates did not significantly affect ruminal pH and ammonia N concentration. Total VFA concentration, VFA molar proportions and blood metabolites were also unaffected by PBS replacement. These results suggest that substitution of PBS up to 27% of diet dry matter did not significantly increase methane production and was equal or superior to concentrates in ADF digestibility and nitrogen retention for growing steers.