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Gwak, Woo-Seok,Zhang, Qun,Roy, Animesh The Ichthyological Society of Korea 2019 韓國魚類學會誌 Vol.31 No.4
This study was conducted to know the genetic diversity and population structure of Japanese halfbeak (Hyporhamphus sajori) in the Northeast Asia, using mitochondrial DNA control region. In the present study, a total of 70 individuals were collected from three locations of China (Liaoning), Korea (Tongyeong) and Japan (Wakasa Bay), and 47 individuals sequences from three locations of Japan (Wakasa Bay, Toyama Bay and Mikawa Bay) were downloaded from genbank. A total of 7 haplotypes were identified with 7 polymorphic sites from 358 bp length sequences. Haplotype and nucleotide diversity were very low and ranged from 0 to 0.295±0.156 and 0 to 0.0009±0.0011, respectively. Ancestral haplotype was shared by 94% individuals. An extremely low haplotype and nucleotide diversity, and starlike minimum spanning tree indicated that the species have undergone a recent population expansion after bottleneck. Pairwise F<sub>ST</sub> values were low and there was no significant differences among populations suggesting a gene flow among the populations. Dispersal of the eggs with the aid of drifting seaweed and currents might be the major responsible factor for the genetic homogeneity. 학꽁치(Hyporhamphus sajori)의 유전적 다양성과 집단구조를 조사하기 위해 동북아시아에서 시료를 채집하여 mitochondrial DNA control region (mtDNA CR)을 분석하였다. 시료는 중국(Liaoning), 한국(통영), 일본(Wakasa Bay) 3곳에서 총 70개체를 채집했고, 일본 3곳(Wakasa Bay, Toyama Bay and Mikawa Bay)에서 분석된 47개체의 mtDNA CR 염기서열을 Genbank에서 다운로드했다. 분석결과 총 358 bp가 나타났고, 7개의 변이와 함께 haplotype이 7개 확인되었다. Haplotype diversity과 nucleotide diversity는 각각 0~0.295±0.156 및 0~0.0009±0.0011이고, main haplotype을 94%의 개체가 공유했다. 매우 낮은 haplotype diversity와 nucleotide diversity 그리고 starlike minimum spanning tree는 집단이 최근에 병목현상을 거친 후, 팽창되었음을 나타낸다. 집단 간에 Pairwise F<sub>ST</sub> 값은 낮고 유의하지 않은 것으로 나타났고, 이것은 집단 간 gene flow가 있음을 시사한다. 학꽁치의 genetic homogenity는 부유조와 해류가 주요 원인으로 생각된다.
Gwak Woo-Seok,Tanaka Masaru The Korean Society of Fisheries and Aquatic Scienc 2004 Fisheries and Aquatic Sciences Vol.7 No.4
The effects of temperatures, starvation, and kind of foods on growth, RNA/DNA ratio and protein contents during metamorphosis and early juvenile stage of olive flounder (Paralichthys olivaceus) were examined. During metamorphosis, warm-acclimated fish showed higher RNA and DNA content than those of the cold-acclimated fish, excepting H stage (28 DAH) at which the ratio was higher at cold temperature. RNA/DNA ratio during metamorphosis showed similar values at two temperatures tested. However, after 42 DAH warm-acclimated juveniles had higher DNA content compared with cold-acclimated fish, resulted in marked decreases in RNA/DNA ratios. Higher RNA content at H stage of cold-acclimated fish was consistent with an increase in protein content. Growth of fish rearing at warm temperature was higher than those of fish at cold temperature during all experiments. In starvation experiment, contents of DNA, RNA and protein significantly decreased. Even though there were no significant differences in total length (TL) and body weight between the live mysid-fed and artificial pellet-fed fish at 35 mm TL, both RNA/DNA and protein/DNA ratios of the former group was significantly higher than those of the latter due primarily to lower DNA content of the live mysid-fed group. The results from this study suggest that temperature, starvation and kind of foods should be considered when RNA/DNA ratio applied to assessing the cultured larval and juvenile fish condition.
Analysis of Phenolic compound and isoflavone in various medicinal soybeans
Jae-woo Kim,Eun-young Kang,Su-hyun Seo,Eun-hye Kim,Tae-sic Gwak,Hyun-seok Jeon,Joung-kuk Ahn,Woo-seok Jung,Ill-min Chung 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Medicinal soybean, which has been proven to be effective in preventing cancer, heart disease, osteoporosis, and renal failure, is drawing people’s attention among other beans in recent days. This experiment was to examine the concentration of phenolic compounds and isoflavone in 53 kinds of soybeans cultivated and collected in Yeo-Ju, Gyeonggi-do region. The total average content of phenol is 29424.34㎍/g and the most and least phenol were detected in No. 35(814.98㎍/g) and No. 28(353.66㎍/g) respectively. Myricetin was detected the most resveratrol whereas detected the least amount which was less than 0.2% of the total amount among all medicinal soybean samples. Total average isoflavone content was 35910.56㎍/g and the most and least isoflavone was detected in No. 15 (996.66 ㎍/g ) and No.9 (476.18㎍/g )respectively . Malonylgenistin was detecteded the most genistein whereas detected the least amount which was less than 0.02 % of the total amount in all medicinal soybean samples. The result of this experiment is considered to be the basic data of the functional food manufacturing by utilizing domestic medicinal soybean.
곽우석 ( Woo Seok Gwak ),최병언 ( Byung Eon Chol ),이소광 ( So Gwang Lee ) 한국어류학회 2012 韓國魚類學會誌 Vol.24 No.2
진해만 대구의 산란기 수온, 부화일과 초기성장을 추정하 였다. 2006년부터 2009년 사이 11월에서 5월까지 매월 8개 의 정점의 수온을 측정하여 분석한 결과 주 산란기의 수온 은 7~9˚C였다. 2006~2008년의 주산란기인 12월에서 2월 사이 산란어의 생식소숙도지수(GSI)는 수컷에 비하여 암컷 이 유의하게 컸으며, 1월에 최대값을 보였다. 2008년 5~6 월에 채집된 치어의 전장은 37.5~94.9 mm (63.2±11.0 mm; mean±SD) 범위였으며, 이석의 부화 이후 일륜수의 평균(±SD)은 5월 17일 표본에서는 102±15, 5월 29일에 는 119±16, 6월 3일에는 116±18였다. 이석의 일륜수로부 터 추정한 부화일은 12월 하순에서 3월 중순이었으며, 주 부화 시기는 1월 하순에서 2월 중순이었다. 부화 이후 일 령(t)에 대한 전장(TL, mm)의 성장은 Gompertz 성장식, TLt=123.2 exp{-exp[-0.0165(t-81.814)]} (r2=0.93, n= 273)로 나타낼 수 있었다. Hatching date and early growth of Pacific cod Gadus macrocephalus were estimated by examination of otolith microstructure of Pacific cod juveniles collected in Jinhae Bay of Korea from May to June, 2008. Water temperature during the main spawning time ranged from 7 to 9˚C according to the geographic distribution of temperature measured from November to May between 2006 and 2009. The spawners were collected from December to February between 2006 and 2008, and the gonadosomatic index of spawners was larger in females than in males, showing a peak in January. Total length of juveniles ranged from 37.5 to 94.9 mm (63.2±11.0 mm; mean±SD). The number of daily growth increments in juvenile otoliths were on average 102±15 in May 17 samples, 119±16 in May 29 ones and 116±18 in June 3 ones. Hatching dates estimated from the number of daily growth increments ranged from late December to mid-March, showing the peak between late January and mid-February. Daily growth in total length (TL, mm) can be adjusted to the Gompertz curve: TLt= 123.2exp {-exp [-0.0165(t- 81.8)]} (r2=0.93, n=273).