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      • KCI등재

        Enhancement Effects of Urea-Phosphate, Paclobutrazol, Indole-3-Butyric Acid, and Their Combinations on Rooting of Neem (Azadirachta indica) Stem Cuttings

        Gholamreza Abdi,Mohammad Hedayat 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.3

        Neem (Azadirachta indica) is an important tropical tree species with many uses. To improve the rooting response of neem cuttings, urea-phosphate (UP) and paclobutrazol (PBZ) were tested in combination with indole-3-butyric acid (IBA). UP alone did not stimulate rooting of neem cuttings; however, when applied together with IBA it significantly enhanced the rooting of cuttings. PBZ alone had a weak effect on rooting of cuttings, but in combination with IBA it improved the rooting of cuttings. A triple combination of IBA, UP, and PBZ provided the highest effect on the improvement of rooting percentage. IBA treatments increased the number of roots per cutting in comparison with the control, but decreased the root length of cuttings. IBA plus UP or PBZ further increased the number of newly formed roots. In addition, IBA plus UP increased the average root length. The combined effect of the three compounds on root number per cutting was not different from that of IBA plus PBZ, but the roots were longer than in the IBA-treated and the control cuttings. The survival of rooted cuttings treated with IBA was relatively low. The triple combination of IBA, UP, and PBZ improved the survival of the rooted neem plants compared with IBA alone. Thus, UP and PBZ were shown to enhance the effect of IBA in stimulation of rooting and survival of neem cuttings.

      • KCI등재

        Callus Induction and Plant Regeneration in Tanacetum cinerariaefolium (Trevir.) Schultz-Bip: an Important Medicinal Plant

        Gholamreza Abdi,Mohammad Hedayat 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.4

        Pyrethrum is an important medicinal perennial herb belonging to the family Asteraceae. A method for rapid micropropagation of pyrethrum through plant regeneration from leaf and petiole explant derived calli has been developed. The petiole and leaf segments were cultured on MS, SH and B5 media supplemented with the combination of auxins and BA for callus induction. All petiole explants on SH medium containing 2 ㎎ㆍL?¹ NAA and 0.2 ㎎ㆍL?¹ BA produced callus. The highest rate of callus growth was observed with the MS medium supplemented with 0.6 ㎎ㆍL?¹ BA and 4 ㎎ㆍL?¹ NAA. Shoot regeneration was obtained successfully by using step-by-step method. Firstly, callus was subcultured on MS medium containing 0.2 ㎎ㆍL?¹ 2,4-D and then, the calli were transferred to MS medium containing 4 ㎎ㆍL?¹ BA, 1 ㎎ㆍL?¹ NAA and 0.5 ㎎ㆍL?¹ folic acid. In the latter step, some protuberances appeared on compact calli. These protuberances produced shoots on MS media containing 1 ㎎ㆍL?¹ BA and 1 ㎎ㆍL?¹ NAA or 1 ㎎ㆍL?¹ BA and 2 ㎎ㆍL?¹ NAA and 0.4 ㎎ㆍL?¹ GA₃. The optimal rooting response was observed on B5 medium supplemented with 2 ㎎ㆍL?¹ NAA, on which 100% of the regenerated shoots developed roots with an average of 16 roots per shoot within 3 weeks. The plantlets were acclimatized and transferred to the greenhouse with 80% survival. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this medicinal plant.

      • KCI등재

        Effect of Natural Zeolite and Paclobutrazol on Reducing Salt Stress in Kentucky Bluegrass (Poa pratensis L.)

        Gholamreza Abdi,Hassan Salehi,Saeid Eshghi 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

        A greenhouse experiment was conducted to study the effect of zeolite and drench application of paclobutrazol (PBZ) on the salt stress response. Kentucky bluegrass (Poa pratensis L.) turfs were grown in boxes filled with sand (100%), sand + 15% zeolite, sand + 30 ㎎ PBZ, and sand + 15% zeolite + 30 ㎎ PBZ, and were irrigated with 0.24 (control irrigation), 3.4 and 6.4 dS · m?¹ saline water daily for 6 months in greenhouse condition. Saline water reduced TQ, TFW, TDW, CY, PR, transpiration, SC, ME, and WUE, compared to control condition, Paclobutrazol and salinity treatments reduced growth and development, progressively produced plant that were too small especially at the highest saline concentration. Amendment of sand with zeolite increased TQ, TFW, TDW, CFW, CDW, PR, transpiration, SC, ME, and WUE during both second and third months of the experiment at both salinity levels. The beneficial effects of zeolite on turf quality and other parameters diminished 3 months after treatment. Amendment of sand with zeolite reduced leaching of Na and K but increased leaching of Ca?? and Mg??. Using zeolite in medium increased SAR value compared to control. Results indicated that amending with zeolite may buffer soil solution Na+ concentration in short-term. In the long-term, however, a substantial amount of Na? may be retained concurrent with Ca?? and Mg?? exchange, thereby increasing sodicity and salinity problems. Furthermore, results indicated that PBZ can reduce salinity effect via reducing the availability of salt ions in the medium.

      • KCI등재

        Turf Seedling Height and Quality in Paclobutrazol-treated Seeds of Lolium perenne L. ‘Barbal’ Sown in the Soil Mixed with Zeolite

        Mahsa Shahrokhi,Hassan Salehi,Saeid Eshghi,Gholamreza Abdi 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.6

        To determine the effect of paclobutrazol on seedling height and visual quality, seeds of Lolium perenne L. ‘Barbal’ were soaked with 15, 30 and 45 ㎎?L<SUP>-1</SUP> paclobutrazol for 24 h on shaker during imbibitions stage of seed germination. Treated seeds were potted in the soil mixtures containing 5, 10, 15 and 30 percent zeolite with gardening soil and sand. 30 ㎎?L<SUP>-1</SUP> paclobutrazol followed by 15% zeolite resulted in the lowest seedling height. After the first mowing, lower heights were obtained in 30 ㎎?L<SUP>-1</SUP> paclobutrazol with 30% zeolite and in 45 ㎎?L<SUP>-1</SUP> paclobutrazol without zeolite. Paclobutrazol and zeolite combined at the highest concentrations resulted in the lowest height and clipping dry weight at second mowing. Treatment of 5 % zeolite had lower color quality but paclobutrazol at concentration of 15 ㎎?L<SUP>-1</SUP> produced the highest turf quality. It can be concluded that using zeolite in the media can lower the height of seedlings previously treated with paclobutrazol.

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