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G. Satheesh kumar,T. Kiran Reddy,B. Madhavi,P. Charan Teja,M. Subhosh Chandra,Yong Lark Choi 한국생명과학회 2010 생명과학회지 Vol.20 No.5
폐식용유에서 지방분해효소를 생산하는 세균을 분리하였고, PIBWIN 세균동정 방법으로 생리 생화학적 특성을 조사하여 확인한 결과 Pseudomonas sp. OME로 동정하였다. 여러 기질로 지방분해효소 생산을 조사한 결과 올리브유에서 6.1 U/㎖의 생산력을 나타내었다. 물리적 인자인 배양시간, 온도. pH 및 올리브유와 효모 추출액의 영양인자에 의한 지방분해효소 생산 조건을 조사 하였다. 효소의 분비는 배양시간. 올리브유 와 효모 추출액의 농도에 강한 영향을 받았으며, RSM을 이용한 최적화는 이들 인자를 가지고 조사하였다. RSM을 이용한 지방분해효소 생산은 배양시간. 올리브유와 효모 추출액의 농도가 48 hr, 0.3 g, 및 0.9 ㎖에서 최적 생산조건을 나타냈다. Lipolytic enzyme-producing bacteria were isolated from edible oil mill effluents on tributyrin agar medium. The shake-flask-scale studies yielded a promising isolate and it was identified as Pseudomonas sp. An OME using various microbiological observations such as cultural, microscopic, and biochemical tests was undertaken and confirmed using PIBWIN bacterial identification software. Lipolytic enzyme production was screened with oils such as sunflower, caster, coconut, tributyrin, and olive. Amongst these, olive oil showed an increased lipase production 6.1 U/㎖. In view of the highest lipolytic enzyme production with olive oil, further optimizations were carried out using olive oil as a carbon source. Lipolytic enzyme production was optimized by a conventional ‘one variable at a time’ approach and the significant factors were further analyzed statistically using response surface methodology (RSM). The effect of physical factors such as incubation time, temperature, initial medium pH, and nutritional factors such as concentration of olive oil and yeast extract were examined for lipase production. Lipolytic enzyme secretion was strongly affected by three variables (incubation time, concentration of yeast extract and olive oil). Therefore, the interaction of these three factors was further optimized using response surface methodology. The optimized conditions of lipase production using response surface methodology yielded a maximum of 9.62 U/㎖ with optimum conditions for incubation, yeast extract and olive oil concentrations were found to be 48 hr, 0.3 g. and 0.9 ㎖. respectively.
G. Satheesh kumar,P. Sreenivasulu,M. Subhosh Chandra,Yong-Lark Choi,K. V. Mallaiah 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.3
In this study, the production of extracellular thermostable α-amylase by newly isolated thermophilic Alicyclobacillus acidocaldarius was detected on LB agar plates containing 1.0% soluble potato starch and incubated at 60oC. This extracellular α-amylase was purified to homogeneity by ammonium sulphate precipitation followed by Sephadex and ion-exchange chromatography. The α-amylase was purified to 8.138 fold homogeneity with a final recovery of 58% and a specific activity of 3,239 U/mg proteins. The purified α-amylase appeared as a single protein band on SDS-PAGE with a molecular mass of 94.5 kDa. Non-denaturing PAGE analysis showed one major band associated with enzyme activity, indicating the absence of isoenzymes. A TLC analysis showed maltose as major end product of the enzyme. The optimum assay temperature and pH for enzyme activity were 60oC and 6.0respectively; however, the enzyme activity was stable over a wide range of pH and temperatures. The α-amylase retained its activity in the presence of the denaturing agents - SDS, Triton X-100, Tween-20, Tween-80, and was significantly inhibited by EDTA and urea. Calcium ions increased the enzyme activity, while Hg2+, Zn2+, and Co2+had inhibitory effects. The Km and Vmax values were found to be 2.9 mg/mL and 7936 U/mL respectively.
Kumar, G. Satheesh,Chandra, M. Subhosh,Sumanth, M.,Vishnupriya, A.,Reddy, B. Rajasekhar,Choi, Yong-Lark The Korean Society for Applied Biological Chemistr 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.1
Newly isolated strains Bacillus sp. FME 1 and FME 2 were evaluated for the cellulolytic enzymes production during submerged fermentation (SmF) of different substrates including rice husk, Whatman filter paper and cellulose powder CF 11. Extracellular enzyme assays for CMCase, FPase and ${\beta}$-glucosidase were examined up to 8 days of submerged fermentation. Among the three substrates, rice husk was the most suitable substrate for higher production of cellulolytic enzymes. Maximum titers of 100, 45, and 3.5 U/mL in respect of CMCase, FPase and ${\beta}$-glucosidase in Bacillus sp. FME 2 were recovered as against 45, 12, and 0.39 U/mL in Bacillus sp. FME 1 respectively, at their respective peak time intervals. Bacillus sp. FME 2 was found to produce higher cellulolytic enzyme activities than Bacillus sp. FME 1.
G. Satheesh Kumar,M. Subhosh Chandra,M. Sumanth,A. Vishnupriya,B. Rajasekhar Reddy,Yong-Lark Choi 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.1
Newly isolated strains Bacillus sp. FME 1 and FME 2 were evaluated for the cellulolytic enzymes production during submerged fermentation (SmF) of different substrates including rice husk, Whatman filter paper and cellulose powder CF 11. Extracellular enzyme assays for CMCase, FPase and β-glucosidase were examined up to 8 days of submerged fermentation. Among the three substrates, rice husk was the most suitable substrate for higher production of cellulolytic enzymes. Maximum titers of 100, 45, and 3.5 U/mL in respect of CMCase, FPase and β-glucosidase in Bacillus sp. FME 2 were recovered as against 45, 12, and 0.39 U/mL in Bacillus sp. FME 1 respectively, at their respective peak time intervals. Bacillus sp. FME 2 was found to produce higher cellulolytic enzyme activities than Bacillus sp. FME 1.
G. Satheesh Kumar(사티시 쿠마르),M. Subhosh Chandra(수보쉬 찬드라),K. V. Mallaiah(브이 말라이아),P. Sreenivasulu(스리니바슐루),Yong Lark Choi(최용락) 한국생명과학회 2012 생명과학회지 Vol.22 No.4
고온성 α-아밀라제를 생산하는 내열성 Alicyclobacillus acidocaldarius 균을 인도 Tirupati, Andhra Pradesh 지역의 가열한 미강 열수 추출물에서 분리하였다. 분리균인 내열성 Alicyclobacillus acidocaldarius가 생산하는 세포 외 α-아밀라제의 생산과 성장에 미치는 배양조건을 실험실 규모로 조사하였다. 그 결과 α-amylase의 고생산 최적 조건은 온도 60oC, pH 6.0 및 배지의 전분농도 1.0%, yeast extract와 tryptone은 0.2%를 나타냈다. Surfactantslike Tween-20과 SDS 같은 계면활성제는 0.02%까지 균주의 성장과 효소 생산을 증가 시켰으나, 그 이상의 농도에서는 α-amylase 효소의 생산이 현저하게 감소하였다. A thermophilic Alicyclobacillus acidocaldarius, which produces thermostable α-amylase, was isolated from the hot water effluent of a boiled rice mill near Tirupati, Andhra Pradesh, India. The effect of different culture conditions on the growth and production of extracellular α-amylase by thermophilic A. acidocaldarius was investigated in laboratory scale. The results showed that the optimum conditions for the production of α-amylase are a temperature of 60°C, pH of 6.0, and medium starch concentration of 1.0%, and yeast extract and tryptone of 0.2%. Surfactants, like Tween-20 and SDS, up to 0.02%, were found to increase the bacterial growth and enzymes. Further increase in their concentration resulted in significantly decreased enzyme production.
ON STRONGLY REGULAR NEAR-SUBTRACTION SEMIGROUPS
Dheena, P.,Kumar, G. Satheesh Korean Mathematical Society 2007 대한수학회논문집 Vol.22 No.3
In this paper we introduce the notion of strongly regular near-subtraction semigroups (right). We have shown that a near-subtraction semigroup X is strongly regular if and only if it is regular and without non zero nilpotent elements. We have also shown that in a strongly regular near-subtraction semigroup X, the following holds: (i) Xa is an ideal for every a $\in$ X (ii) If P is a prime ideal of X, then there exists no proper k-ideal M such that P $\subset$ M (iii) Every ideal I of X fulfills $I=I^2$.
WEAKLY PRIME LEFT IDEALS IN NEAR-SUBTRACTION SEMIGROUPS
Dheena, P.,Kumar, G. Satheesh Korean Mathematical Society 2008 대한수학회논문집 Vol.23 No.3
In this paper we introduce the notion of weakly prime left ideals in near-subtraction semigroups. Equivalent conditions for a left ideal to be weakly prime are obtained. We have also shown that if (M, L) is a weak $m^*$-system and if P is a left ideal which is maximal with respect to containing L and not meeting M, then P is weakly prime.