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NANOSIZED HYDROXYAPATITE/COLLAGEN COMPOSITE
Feng, Feng, Q,L,Cui, Cui, F.Z,Wang, Wang, R.Z,Du, Du, C,Li, Li, H.D 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.1 No.2
This paper reported the synthesis of a nano-Hydroxyapatite(HAp)/collagen biocomposite and its biological performances. A thoroughly mixed slurry of type I collagen in supersaturated HAp solution was obtained. HAp was then controlled to precipitate on collagen by adjusting the solution properly. SEM, XRD and TEM analysis reveals that HAp in the composite was nanometer sized and uniformly dispersed oncollagen matrix. In vitro biocompatibility of this new biomaterial was evaluated in terms of its biodegradation and bioactivity. The mice macrophage and osteoblast were used for the study, and the behavior of the cells in contact with the composite was investigated by means of SEM. The observations showed that the composite can be degraded through extracellular resorption process mediated by macrophage, and they can also be well-contact with osteoblast cells, in vitro.
An Orbital Design Method for Satellite Formation Flying
Cui Hai-Ying,Li Jun-Feng,Gao Yun-Feng The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.2
An orbital design method of the formation initialization based on the relative orbital element method is presented. It firstly constructed the relative motion equation of the satellite formation flying in terms of the leader and followers' orbital elements. Then the equation was simplified when the orbit eccentricity of the leader satellite was small. And according to the satellites' mission, a general design method for the relative trajectory was proposed. The advantage of this method is that one can get a very simple analytical formula of each follower satellite's orbital elements when the orbital eccentricity of the leader satellite is zero. The simulation results show that the method is effective.
Cui-Feng Jiang,Zhuojian Li,Yinxing Wu,Jinshan Wang,Qiong Jiang 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.5
Hg2+ is a serious environmental contaminant that can cause severe damage to the immune and nervous systems, and Hg2+ monitoring is critical. Herein, we report a novel, simple, and sensitive Hg2+ sensor based on the enhanced peroxidase?like activity of chitosan?gold nanoparticles (CS?AuNPs). The peroxidase?like activity of the CS?AuNPs can be markedly enhanced by Hg2+ as seen by the deeper color and increased absorbance at 652?nm. This improved catalytic ability was used to develop a sensitive and selective colorimetric sensor for Hg2+ detection. The limit of detection via the absorption spectra is as low as 0.02??M. The mechanism underlying the enhanced catalytic ability is discussed. This approach is label?free, simple, and offers detection with the naked eye. Thus, it has potential applications for the detection of Hg2+ in environmental and food samples.
Cui Feng(崔峰) 원광대학교 법학연구소 2015 圓光法學 Vol.31 No.2
Because of group portrait right's specificity, when one exercising its own personal portrait right, he or she can't neglect the group portrait right. But because of the group portrait right also belongs to the right of portrait, so it also having portrait right's features. Personal portrait whether covered by group portrait, should be based on its identifiable characteristics. Then we could classify the group portrait as following two kinds: having distinct person and don't have distinct person. From the view of portrait right's content and the dispute of group portrait, exercising the group portrait is a right of privacy. But when the right's exercise is aiming at social public benefits, the privacy right should be limited. 集体肖像因形式上的特殊性使得个人肖像的使用不得忽略集体肖像的整体性,但其仍属于肖像的范畴应具备肖像的特点。个人肖像是否被集体肖像涵盖应基于肖像的可辨认性特点判断,基于此可以分为能够辨认突出人物的集体肖像和无法清晰辨认突出人物的集体肖像。 从肖像权的内容与集体肖像使用纠纷的现实纬度看,集体肖像的使用是一种自我决定权的行使,但在以社会公共利益为目的使用时应限制其自我决定权。
미세분진이 흰쥐의 폐포대식세포에서 Nitric Oxide 생성 및 iNOS 발현과 Nitrotyrosilated-protein의 형성에 미치는 효과
최봉희 ( Feng Ji Cui ),리천주 ( Tian Zhu Li ),이수진 ( Soo Jin Lee ),박세종 ( Se Jong Park ),임영 ( Young Lim ),김경아 ( Kyung A Kim ),장병준 ( Byung Joon Chang ),이종환 ( Jong Hwan Lee ),이명헌 ( Myoung Heon Lee ),최농훈 ( Nong 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.60 No.4
연구배경 : 본 연구는 교통량이 많은 서울의 한 대로변에서 채취된 복합적인 미세분진이 염증반응이 일어날 때 형성되는 것으로 알려진 NO와 NO를 형성하는 iNOS발현 및 NO에 의하여 생성이 증가되는 nitro-tyrosilated-protein의 발현에 미치는 영향을 알아보고자 SPE 흰쥐와 염증성 흰쥐에서 분리된 폐포대식세포와 그람음성 세균의 세포벽 성분인 LPS에 감작시켜 분석하였다. 방법 : SPE 흰쥐와 염증성 흰쥐의 폐포대식세포에서 PM을 농도별로 처리하였을 때와 동일한 농도에서 배양시간을 달리하였을 때 분비되는 NO를 Griess reaction 방법으로 측정하였고, iNOS와 nitrotyrosilated-protein의 발현을 세포면역화학염색법과 western blot 분석법으로 확인하였다. 결과 : PM의 단독투여 및 LPS와 PM을 혼합하여 투여하였을 때 NO의 생성 및 iNOS와 nitrotyrosilated-protein의 발현을 확인할 수 있었고, 그 효과는 LPS를 단독투여 하였을 때보다 발현이 증가함을 확인할 수 있었다. 한편, 폐 내 자연적 염증성 증상이 있는 흰쥐에서 분리된 폐 내 대식세포는 PM의 농도증가에 따라 비례하여 NO의 형성을 증가시켰다. 결론 : 본 실험에 사용된 PM은 그 자체만으로도 SPF 흰쥐와 폐렴증상이 있는 흰쥐 BAL cell에서 NO의 생성을 증가시켰고, LPS가 처리된 세포들에 PM을 병용하여 투여하였을 경우에는 NO의 생성과 iNOS와 nitrotyrosilated-protein의 발현이 유의하게 상승되었다. Background : Particulate matters (PM) when inhaled is known to induce pulmonary diseases including asthma and chronic bronchitis when inhaled. Despite the epidemiological proofevidence, the pathogenesis of PM-related pulmonary diseases is unclearremain poorly understood. Methods : Primary alveolar macrophages were harvested from the SPF and inflammatory rats by bronchioalveolar lavage (BAL). The cultured primary alveolar macrophages were treated with the medium only, PM only (5~40㎍/㎠), LPS (5ng/ml) only, and PM with LPS for 24 and 48 hours. The level of secreted nitric oxide (NO) was assayed from the cultured medium by using the Griess reaction. The cultured cells were utilized for the western blotting against the inducible nitric oxide synthase (iNOS) proteins. Immunocyto- chemical staining against the iNOS and NT-proteins were performed in cells that cultured in the Lab-Tek(R) chamber slide after treatments. Results : The PM that utilizein this experiments induced NO formation with iNOS expression in the cultured SPF and inflammatory rats alveolar macrophages, by itself. When the cells were co-treated with PM and LPS, there was a statistically significant synergistic effect on NO formation and iNOS expression over the LPS effect. The cells from the sham control showed minimal immunoreactivity for the NT-proteins. Significantly higher quantities of NT-proteins were detected in the PM and PM with LPS co-treated cells than from the sham control. Conclusion : Increased iNOS expression and NO formation with increased NT-proteins formation might be involved in the pathogenesis of PM-induced lung injury. (Tuberc Respir Dis 2006; 60: 426-436)