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Chunyu Zhang,박인애,Fangsen Xu,Maoteng Li,임용표,Jinling Meng 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.4
Lacking P protein of glycine decarboxylase (GDCP) from the mesophyll cells was one of the major steps for C_3species to evolve into C_3-C_4 species. Previous studies indicated that the lack of P protein in the mesophyll cells of C_3-C_4species of Flaveria is regulated by gene different transcription. In the family of Brassicaceae, most plants show a typical C_3photosynthetic characteristic, which includes some important vegetables and oil crops, while few plants in this family exhibit a C_3-C_4 type of character. To understand the mechanism of difference in distribution of P protein between the 2 different photosynthetic types, a C_3 type of 1.6 kb BnGDCP promoter from Brassica napus was used for detailed analysis in this study. This promoter exhibited the ability to drive beta-glucuronidase gene (GUS) expression in both mesophyll and the bundle sheath cells of C_3 species, Arabidopsis. However, the same promoter was also found to drive GUS expression in both the mesophyll and bundle sheath cells of a typical of C_3-C_4 species such as Moricandia arvensis, which loses the P protein from the mesophyll cells. This implies that in absence of P protein from the mesophyll cells of Moricandia (C_3-C_4 species) may be regulated by differential transcription of the P protein gene as well. And then, a region, which determines a mesophyll cells specific expression, was narrowed down to 135 bp in length through detailed promoter/reporter gene assay. DNA sequences alignment of the 5′-flanking sequences from either a C3 or C_3-C_4 species in Brassicaceae indicated that 2 different nucleotide acids only conserved to C_3 species were revealed. Phylogenetic analysis of those 5′-flanking sequences of GDCP indicated that C_3-C_4 species in the genus of Moricandia might have been evolved from different C_3 ancestors; interestingly, a C_3-C_4species, D. tenuifolia, was indicated to have shared common ancestors with the C_3-C_4 species, M. spinosa, and the C_3 species M. foleyii in Moricandia.