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Han, Jeonghoon,Kim, Duck-Hyun,Kim, Hui-Su,Kim, Hee-Jin,Declerck, Steven A.J.,Hagiwara, Atsushi,Lee, Jae-Seong Elsevier 2018 Comparative biochemistry and physiology. Part D, G Vol.25 No.-
<P><B>Abstract</B></P> <P>While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 <I>CYP</I> genes in the freshwater rotifer <I>Brachionus calyciflorus</I>. The 31 <I>Bc-CYP</I> genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers <I>Brachionus plicatilis</I> and <I>Brachionus koreanus</I>. To understand the molecular responses of these 31 <I>Bc-CYP</I> genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three <I>Bc-CYP</I> genes (<I>Bc-CYP3044B3</I>, <I>Bc-CYP3049B4</I>, <I>Bc-CYP3049B6</I>) were significantly upregulated (<I>P</I> <0.05) in response to B[α]P, suggesting that these <I>CYP</I> genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in <I>B. calyciflorus</I>. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.</P>
Seo, Woo Duck,Yuk, Heung Joo,Curtis-Long, Marcus J.,Jang, Ki Chang,Lee, Jin Hwan,Han, Sang-Ik,Kang, Hang Won,Nam, Min Hee,Lee, Sung-Joon,Lee, Ji Hae,Park, Ki Hun American Chemical Society 2013 Journal of agricultural and food chemistry Vol.61 No.5
<P>Adenosine 5′-monophosphate-activated protein kinase (AMPK) is an intracellular sensor that can regulate glucose levels within the cell. For this reason, it is well-known to be a target for drugs against diabetes and obesity. AMPK was activated significantly by the hexane extract of barley sprouts. This AMPK activation emerges across the growth stages of the sprout, becoming most significant (3 times above the initial stages) 10 days after sprouting. After this time, the activation decreased between 13 and 20 days post-sprouting. Analysis of the hexane extracts by gas chromatography–mass spectrometry showed that the amounts of policosanols (PCs, which are linear, primary aliphatic alcohols with 20–30 carbons) in the plant dramatically increased between 5 days (109.7 mg/100 g) and 10 days (343.7 mg/100 g) post-sprouting and then levels fell back down, reaching 76.4 mg/100 g at 20 days post-sprouting. This trend is consistent with PCs being the active ingredient in the barley plants. We validate this by showing that hexacosanol is an activator of AMPK. The richest cultivar for PCs was found to be the Daejin cultivar. Cultivars had a significant effect on the total PC content (113.2–183.5 mg/100 g) within the plant up to 5 days post-sprouting. However this dependence upon the cultivar was not so apparent at peak stages of PC production (10 days post-sprouting). The most abundant PC in barley sprout, hexacosanol, contributed 62–80% of the total PC content at every stage. These results are valuable to determine the optimal times of harvest to obtain the highest yield of PCs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2013/jafcau.2013.61.issue-5/jf3041879/production/images/medium/jf-2012-041879_0004.gif'></P>
Sang-Ik Han,Ki Chang Jang,Woo Duck Seo,Ji-Eun Ra,Jun Young Kim,Seong-Hwan Oh,Kyung-Jin Choi,Min-Hee Nam 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Sorghum is the fifth most important cereal in the world as one of the staple food. For the use of natural dye, we have done some researches about sorghum red pigments extracted from stalk and leaves on its physiochemical properties, extracting methods and applications. The researches involved maximum extraction of sorghum pigment and analysis of its processing condition. Total polyphenol and tannin contents were measured by varieties and different plant parts. The stabilities of pigment by irradiation and heat treatment for processing were measured by colorimeter and thermal gravimetric analysis (TGA). In addition, hybrid nano-silica composites with sorghum pigment were made by combining with polyvinyl alcohol, polyvinyl acetate and sodium silicate. Water silica hybrids with sorghum pigment were performed by emulsion treatment. Nano-silica particles were identified and measured their size to be about 200 ~ 400 nm by SEM analysis.
Lee, Jae-Duck,Yao, Chao-Liang,Capdevielle, Francoise J.,Han, Bao-Cheng,Bear, John L.,Kadish, Karl M. Korean Chemical Society 1993 Bulletin of the Korean Chemical Society Vol.14 No.2
The neutral, reduced, and oxidized 2,2-trans isomers of $Rh_2(ap)_4$ (ap=2-anilinopyridinate) were investigated with respect to dioxygen binding in $CH_2Cl_2$ containing 0.1 M tetrabutyl-ammonium perchlorate. $Rh_2(ap)_4$ binds dioxygen in nonaqueous media and forms a $Rh^{II}Rh^{III}$ superoxide complex, $Rh_2(ap)_4(O_2)$. This neutral species was isolated and is characterized by UV-visible and IR spectroscopy, mass spectrometry and cyclic voltammetry. It can be reduced by one electron at $E_{1/2}$ = -0.45 V vs. SCE in $CH_2Cl_2$ and gives ${[Rh_2(ap)_4(O_2)]}^-$ as demonstrated by the ESR spectrum of a frozen solution taken after controlled potential reduction. The superoxide ion in ${[Rh_2(ap)_4(O_2)]}^-$ is axially bound to one of the two rhodium ions, both of which are in a +2 oxidation state. $Rh_2(ap)_4(O_2)$ can also be stepwise oxidized in two one-electron transfer steps at $E_{1/2}$ = 0.21 V and 0.85 V vs. SCE in $CH_2Cl_2$ and gives ${[Rh_2(ap)_4(O_2)]}^+$ followed by ${[Rh_2(ap)_4(O_2)]}^{2+}$. ESR spectra demonstrate that the singly oxidized complex is best described as ${[Rh^{II}Rh^{III}(ap)_4(O_2)]}^+$ where the odd electron is delocalized on both of the two rhodium ions and the axial ligand is molecular oxygen.
미셀형성을 이용한 한외여과막에서의 코발트(Co), 마그네슘(Mg)이온 제거
김영호,양현수,한광희,강덕원,송명재 한국화학공학회 1996 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.34 No.4
음이온계면활성제는 임계미셀농도 이상에서 소수성 효과에 의해 미셀이 형성되고, 미셀표면의 정전기적인 인력에 의해 양이온 금속이온과 흡착하거나 결합하여 거대분자를 형성하였다. 그리고 이 수용성 흐름을 미셀이나 흡착된 금속 양이온의 투과를 막기에 기공이 충분히 작은 한외여과막에 통과시켰다. 본 연구에서는 막투과압력차는 금속제거에 비교적 작은 영향을 미치는 반면 계면활성제와 금속의 비(S/M)는 상당한 영향을 미쳤다. 또한 음이온계면활성제 SDS는 Mg이온보다 Co이온에 대하여 친화력이 큼을 알 수 있었으며 Co이온의 제거율은 95%로 관찰되었다. The anionic surfactant formed highly charged aggregates called micelles above the CMC, and formed macromolecules by which the metal cations adsorbed or binded. The aqueous stream was then passed through an ultrafiltration membrane with pores small enough to block the passage of the micelles and adsorbed metal cations. In this study, experimental results showed that transmembrane pressure had a relatively small effect on metal removal whereas the level of surfactant-to-metal ratio(S/M) had a substantial effect. The SDS in this study showed higher affinity of Co ion than that of Mg ion. The rejection coefficient of Co was observed as 95%.
Identification and molecular properties of SUMO-binding proteins in Arabidopsis
Park, Hyeong Cheol,Choi, Wonkyun,Park, Hee Jin,Cheong, Mi Sun,Koo, Yoon Duck,Shin, Gilok,Chung, Woo Sik,Kim, Woe-Yeon,Kim, Min Gab,Bressan, Ray A.,Bohnert, Hans J.,Lee, Sang Yeol,Yun, Dae-Jin Springer-Verlag 2011 Molecules and cells Vol.32 No.2