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Deng, Jian-Wei,Kim, Kwon-Bok,Song, Im-Sook,Shon, Ji-Hong,Zhou, Hong-Hao,Liu, Kwang-Hyeon,Shin, Jae-Gook John Wiley Sons, Ltd. 2008 Biomedical chromatography Vol.22 No.2
<P>We developed a method for determining pravastatin or pitavastatin, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, in plasma using liquid chromatography and tandem mass spectrometry (LC–MS/MS). Pravastatin, pitavastatin and the internal standard fluvastatin were extracted from plasma with solid-phase extraction columns and eluted with methanol. After drying the organic layer, the residue was reconstituted in mobile phase (acetonitrile:water, 90:10, v/v) and injected onto a reversed-phase C<SUB>18</SUB> column. The isocratic mobile phase was eluted at 0.2 mL/min. The ion transitions recorded in multiple reaction monitoring mode were m/z 423 → 101, 420 → 290 and 410 → 348 for pravastatin, pitavastatin and fluvastatin, respectively. The coefficient of variation of the assay precision was less than 12.4%, the accuracy exceeded 89%. The limit of detection was 1 ng/mL for all analytes. This method was used to measure the plasma concentration of pitavastatin or pravastatin from healthy subjects after a single 4 mg oral dose of pitavastatin or 40 mg oral dose of pravastatin. This is a very simple, sensitive and accurate analytic method to determine the pharmacokinetic profiles of pitavastatin or pravastatiny. Copyright © 2007 John Wiley & Sons, Ltd.</P>
A Novel Alternaria Species Isolated from Peucedanum japonicum in Korea
( Jian Xin Deng ),( Hye Sun Cho ),( Narayan Chandra Paul ),( Hyang Burm Lee ),( Seung Hun Yu ) 한국균학회 2014 Mycobiology Vol.42 No.1
We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis andtwo protein-coding genes(gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.
Alternaria brassicifolii sp. nov. Isolated from Brassica rapa subsp. pekinensis in Korea
( Jian Xin Deng ),( Mei Jia Li ),( Narayan Chandra Paul ),( May Moe Oo ),( Hyang Burm Lee ),( Sang-keun Oh ),( Seung Hun Yu ) 한국균학회 2018 Mycobiology Vol.46 No.2
A new species belonging to the genus Alternaria was isolated from the necrotic leaf spots of Brassica rapa subsp. pekinensis in Yuseong district, Daejeon, Korea. It is an occasional isolate, not an etiological agent, which is morphologically similar to A. broccoli-italicae, but differs in conidial size and conidiophore shape. Phylogenetic analysis using the sequence datasets of the internal transcribed spacer (ITS) region of the rDNA, glyceraldehyde-3-phosphate dehydrogenase (gpd), and plasma membrane ATPase genes showed that it is distantly related to A. broccoli-italicae and closely related to Alternaria species in the section Pseudoalternaria, which belonged to a clade basal to the section Infectoriae. Morphologically, the species is unique because it produces solitary conidia or conidial chains (two units), unlike the four members in the section Pseudoalternaria that produce conidia as short branched chains. It exhibits weak pathogenicity in the host plant. This report includes the description and illustration of A. brassicifolii as a new species.
Jian Xin Deng,Narayan Chandra Paul,상현규,Ji Hye Lee,황용수,유승헌 한국균학회 2012 Mycobiology Vol.40 No.1
Two species, Penicillium adametzioides and Purpureocillium lilacinum, were isolated from decayed grapes (cv. Cheongsoo) in Korea. Each species was initially identified by phylogenetic analysis of a combined dataset of two genes. Internal transcribed spacer (ITS) and β-tubulin (BT2) genes were used for identification of Penicillium adametzioides, and ITS and partial translation elongation factor 1-α (TEF) genes were used for identification of Purpureocillium lilacinum. Morphologically, they were found to be identical to previous descriptions. The two species presented here have not been previously reported in Korea.
??(Deng, Jian) 한국중어중문학회 2017 中語中文學 Vol.- No.70
This article attempts to review the MO Games from the perspective of magnified “Playbour” with the clue of Arena of Valo. MO Games have become the mainstream games in China after the 21st century. However, the networking is only the surface of this kind of game, while the essence of it is not the pluralization of the players but the transformation of relationship between the gamers and game program. In the process of transformation, MO Games became the digital factory and the latest form of exploitation. Players, on one hand, were trained to be the new species who“amuse ourselves to death”. On the other hand, they were transformed into “Netslaves”. The Playbour advocated in this article is broader and more complex than Modding and Gold Farming, which were called “Playbour” before. In my opinion “Playbour” should be defined as Players’ play action. The logic behind this kind of playbour is the reorganization of time and space that people use to restore labour into the production system of capitalism, and this is the latest development and extension of capitalism in the contemporary era. The characteristics of such kind of playbour are similar to the immaterial labor and digital labor. However, game makers have provided players a framework of consumption by their game mall. In this exact framework, players are trapped in the logic of consumption and resistance to consumption, and are difficult to realize that they are in a larger production system. The situation also obscures the essence of exploitation. A “computer theology” which means that constant capital creates value is displayed to the world: MO Games seem to be an established text created by programmers alone, while the instant text created by players is swept out of the track of value production. Therefore, ludocapitalism is a covert exploitation system disguised as entertainment and consumption. In the end, this article points out the mechanism of interpellation in the playbour. First, MO Games are mathematical texts that interpellate players through the numerical order. Second, MO games not only shape gamer’s consuming consciousness, but also produce their identity. Finally, MO Games use an “addictive mechanism” to interpellate players as labours.
Occurrence of Leaf Blight on Cosmos Caused by Alternaria cosmosa in Korea
Jian Xin Deng,이지혜,Narayan Chandra Paul,조혜선,Hyang Burm Lee,유승헌 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.1
In 2011, a leaf blight disease was observed on cosmos(Cosmos bipinnatus) leaves in Nonsan, Korea. Thecausal pathogen was isolated and identified based onmorphological and molecular approaches. Morphologicalcharacteristics of the pathogen matched well with theAlternaria cosmosa and also easily distinguishablefrom Alternaria zinniae reported from cosmos seedsby producing branched beak. Phylogenetically, thepathogen could not be distinguished from A. passifloraebased on the sequence analysis of a combined dataset of Alt a1 and gpd genes. However, A. passifloraewas distinguished from the present species by havingconidiophores with 4 to 5 conidiogenous loci. Theresults indicate that the present Alternaria species is A. cosmosa. Pathogenicity tests revealed that the isolatewas pathogenic to the leaves of Cosmos bipinnatus. Thisis the first report of Alternaria blight disease caused byA. cosmosa on cosmos in Korea.
Occurrence of Leaf Blight on Cosmos Caused by Alternaria cosmosa in Korea
Deng, Jian Xin,Lee, Ji Hye,Paul, Narayan Chandra,Cho, Hye Sun,Lee, Hyang Burm,Yu, Seung Hun The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.1
In 2011, a leaf blight disease was observed on cosmos (Cosmos bipinnatus) leaves in Nonsan, Korea. The causal pathogen was isolated and identified based on morphological and molecular approaches. Morphological characteristics of the pathogen matched well with the Alternaria cosmosa and also easily distinguishable from Alternaria zinniae reported from cosmos seeds by producing branched beak. Phylogenetically, the pathogen could not be distinguished from A. passiflorae based on the sequence analysis of a combined data set of Alt a1 and gpd genes. However, A. passiflorae was distinguished from the present species by having conidiophores with 4 to 5 conidiogenous loci. The results indicate that the present Alternaria species is A. cosmosa. Pathogenicity tests revealed that the isolate was pathogenic to the leaves of Cosmos bipinnatus. This is the first report of Alternaria blight disease caused by A. cosmosa on cosmos in Korea.
( Jian Xin Deng ),( Narayan Chandra Paul ),( Mei Jia Li ),( Eun Young Seo ),( Gi Ho Sung ),( Seung Hun Yu ) 한국균학회 2011 Mycobiology Vol.39 No.4
Species of Phoma and its allies were isolated during a survey on the diversity of endophytic fungi associated with pine trees in Korea. Based on the phylogenetic analyses of internal transcribed spacer and β-tubulin gene sequences, two Phoma-like species from the needles of Pinus koraiensis were identified as Peyronellaea calorpreferens and P. glomerata. They were also morphologically identified based on the previous descriptions. Here, we report P. calorpreferens and P. glomerata being present in Korea as endophytic fungi in Pinus koraiensis.