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( Narayan Chandra Paul ),( Eom-ji Hwang ),( Sang-sik Nam ),( Hyeong-un Lee ),( Joon-seol Lee ),( Gyeong-dan Yu ),( Yong-gu Kang ),( Kyeong-bo Lee ),( San Go ),( Jung-wook Yang ) 한국균학회 2017 Mycobiology Vol.45 No.3
In this study, we aimed to characterize fungal samples from necrotic lesions on collar regions observed in different sweetpotato growing regions during 2015 and 2016 in Korea. Sclerotia appeared on the root zone soil surface, and white dense mycelia were observed. At the later stages of infection, mother roots quickly rotted, and large areas of the plants were destroyed. The disease occurrence was monitored at 45 and 84 farms, and 11.8% and 6.8% of the land areas were found to be infected in 2015 and 2016, respectively. Fungi were isolated from disease samples, and 36 strains were preserved. Based on the cultural and morphological characteristics of colonies, the isolates resembled the reference strain of Sclerotium rolfsii. Representative strains were identified as S. rolfsii (teleomorph: Athelia rolfsii) based on phylogenetic analysis of the internal transcribed spacer and large subunit genes along with morphological observations. To test the pathogenicity, sweetpotato storage roots were inoculated with different S. rolfsii strains. `Yulmi` variety displayed the highest disease incidence, whereas `Pungwonmi` resulted in the least. These findings suggested that morphological characteristics and molecular phylogenetic analysis were useful for identification of S. rolfsii.
Fungal Endophytes in Roots of Aralia Species and Their Antifungal Activity
Paul, Narayan Chandra,Kim, Won-Ki,Woo, Sung-Kyoon,Park, Myung-Soo,Yu, Seung-Hun The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.4
Endophytic fungi were isolated from surface sterilized root tissues of Aralia elata and Aralia continentalis, collected from farmer's field in Chungnam province, Republic of Korea, in 2005. Based on ITS sequence analysis, 24 fungal genera were characterized from 359 isolates, belonging to 22 Ascomycota, 1 Glomeromycota and 1 Oomycota. Strumella, Rhizopycnis and Entrophospora in A. elata and Rhizopycnis and Leptosphaeria in A. continentalis were the most abundant taxa. Out of 24 genera, Entrophospora, Leptodontidium, Neoplaconema, Paraconiothyrium, Rhizopycnis, Strumella and Tumularia were new to Korea. A total of 110 isolates were tested for antifungal activities against six plant pathogenic fungi. Out of these, 39 isolates showed antifungal activity against at least one plant pathogenic fungi. Four isolates of Pyrenochaeta, 1 isolate of Entrophospora and 1 unidentified fungus strongly inhibited the growth of six plant pathogenic fungi.
( Narayan Chandra Paul ),( Jian Xin Deng ),( Ji Hye Lee ),( Seung Hun Yu ) 한국균학회 2013 Mycobiology Vol.41 No.1
Two new species of endophytic fungi were encountered during a diversity study of healthy tissues of chili pepper plants in Korea. The species were identified as Paecilomyces inflatus and Bionectria ochroleuca based on molecular and morphological analyses. Morphological descriptions of these endophytic isolates matched well with their molecular analysis. In the present study, detailed descriptions of internal transcribed spacer regions and morphological observations of these two fungi are presented.
Narayan Chandra Paul,유승헌,Jian Xin Deng,상현규,최영필 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.1
This study investigated the distribution of endophytic fungi obtained from the surface sterilized tissues of leaves, stems and roots of chili pepper (Capsicum annuum L.) plants in seedling, flowering and fruiting stages in Korea and their antifungal activity. A total of 481isolates were recovered and were identified using molecular techniques. Based on rDNA ITS gene sequence and phylogenetic analysis, 21 fungal genera were characterized, belonging to 16 Ascomycota and 5Basidiomycota. Penicillium in seedling stage, Fusarium in flowering stage, Colletotrichum followed by Fusarium,Alternaria and Xylaria in fruiting stage was predominant and Alternaria, Cladosporium and Fusarium were common in all growth stages. Among 481 endophytes 90 phenotypes were evaluated for the antimicrobial activity against three major pathogens (Phytophthora capsici, Colletotrichum acutatum and Fusarium oxysporum)of chili pepper. Among them 16 isolates inhibited the growth of at least one test microorganisms. Three strains showed a broad spectrum antifungal activity and displayed strong inhibition against chili pepper pathogenic fungi.
Fungal Endophytes in Roots of Aralia Species and Their Antifungal Activity
Narayan Chandra Paul,김원기,우성균,박명수,유승헌 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.4
Endophytic fungi were isolated from surface sterilized root tissues of Aralia elata and Aralia continentalis, collected from farmer’s field in Chungnam province, Republic of Korea, in 2005. Based on ITS sequence analysis, 24 fungal genera were characterized from 359 isolates, belonging to 22 Ascomycota, 1 Glomeromycota and 1 Oomycota. Strumella, Rhizopycnis and Entrophospora in A. elata and Rhizopycnis and Leptosphaeria in A. continentalis were the most abundant taxa. Out of 24 genera, Entrophospora, Leptodontidium, Neoplaconema, Paraconiothyrium, Rhizopycnis, Strumella and Tumularia were new to Korea. A total of 110 isolates were tested for antifungal activities against six plant pathogenic fungi. Out of these, 39 isolates showed antifungal activity against at least one plant pathogenic fungi. Four isolates of Pyrenochaeta, 1 isolate of Entrophospora and 1 unidentified fungus strongly inhibited the growth of six plant pathogenic fungi.
( Narayan Chandra Paul ),( Jian Xin Deng ),( Hyang Burm Lee ),( Seung Hun Yu ) 한국균학회 2015 Mycobiology Vol.43 No.4
In the course of survey of endophytic fungi from Bangladesh pumpkin seeds in 2011~2012, two strains (CNU111042 and CNU111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and Alternaria allergen a1 (Alt a1) sequences. Phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade within the A. alternata clade, matching A. burnsii and showing differences with its other closely related Alternaria species, such as A. longipes, A. tomato, and A. tomaticola. Long ellipsoid, obclavate or ovoid beakless conidia, shorter and thinner conidial size (16~60 [90] × 6.5~14 [~16] μm) distinguish this fungus from other related species. These isolates showed more transverse septation (2~11) and less longitudinal septation (0~3) than did other related species. Moreover, the isolate did not produce any diffusible pigment on media. Therefore, our results reveal that the newly recorded fungus from a new host, Cucurbita maxima, is Alternaria burnsii Uppal, Patel & Kamat.
Paul, Narayan Chandra,Deng, Jian Xin,Sang, Hyun-Kyu,Choi, Young-Phil,Yu, Seung-Hun The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.1
This study investigated the distribution of endophytic fungi obtained from the surface sterilized tissues of leaves, stems and roots of chili pepper ($Capsicum$ $annuum$ L.) plants in seedling, flowering and fruiting stages in Korea and their antifungal activity. A total of 481 isolates were recovered and were identified using molecular techniques. Based on rDNA ITS gene sequence and phylogenetic analysis, 21 fungal genera were characterized, belonging to 16 Ascomycota and 5 Basidiomycota. $Penicillium$ in seedling stage, $Fusarium$ in flowering stage, $Colletotrichum$ followed by $Fusarium$, $Alternaria$ and $Xylaria$ in fruiting stage was predominant and $Alternaria$, $Cladosporium$ and $Fusarium$ were common in all growth stages. Among 481 endophytes 90 phenotypes were evaluated for the antimicrobial activity against three major pathogens ($Phytophthora$ $capsici$, $Colletotrichum$ $acutatum$ and $Fusarium$ $oxysporum$) of chili pepper. Among them 16 isolates inhibited the growth of at least one test microorganisms. Three strains showed a broad spectrum antifungal activity and displayed strong inhibition against chili pepper pathogenic fungi.