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Redditt, Thomas J.,Chung, Eui-Hwan,Karimi, Hana Zand,Rodibaugh, Natalie,Zhang, Yixiang,Trinidad, Jonathan C.,Kim, Jin Hee,Zhou, Qian,Shen, Mingzhe,Dangl, Jeffery L.,Mackey, David,Innes, Roger W. American Society of Plant Biologists 2019 The Plant cell Vol.31 No.11
<P>The <I>Pseudomonas syringae</I> effector protein AvrRpm1 ADP ribosylates RIN4 proteins from Arabidopsis and soybean, which promotes association of RIN4 with EXO70E2 and suppression of callose deposition.</P><P>The <I>Pseudomonas syringae</I> effector protein AvrRpm1 activates the Arabidopsis (<I>Arabidopsis thaliana</I>) intracellular innate immune receptor protein RESISTANCE TO PSEUDOMONAS MACULICOLA1 (RPM1) via modification of a second Arabidopsis protein, RPM1-INTERACTING PROTEIN4 (<I>At</I>RIN4). Prior work has shown that AvrRpm1 induces phosphorylation of <I>At</I>RIN4, but homology modeling indicated that AvrRpm1 may be an ADP-ribosyl transferase. Here, we show that AvrRpm1 induces ADP-ribosylation of RIN4 proteins from both Arabidopsis and soybean (<I>Glycine max</I>) within two highly conserved nitrate-induced (NOI) domains. It also ADP ribosylates at least 10 additional Arabidopsis NOI domain-containing proteins. The ADP-ribosylation activity of AvrRpm1 is required for subsequent phosphorylation on Thr-166 of <I>At</I>RIN4, an event that is necessary and sufficient for RPM1 activation. We also show that the C-terminal NOI domain of AtRIN4 interacts with the exocyst subunits EXO70B1, EXO70E1, EXO70E2, and EXO70F1. Mutation of either EXO70B1 or EXO70E2 inhibited secretion of callose induced by the bacterial flagellin-derived peptide flg22. Substitution of RIN4 Thr-166 with Asp enhanced the association of <I>At</I>RIN4 with EXO70E2, which we posit inhibits its callose deposition function. Collectively, these data indicate that AvrRpm1 ADP-ribosyl transferase activity contributes to virulence by promoting phosphorylation of RIN4 Thr-166, which inhibits the secretion of defense compounds by promoting the inhibitory association of RIN4 with EXO70 proteins.</P><P>[Figure]</P>
A benchmark study on the thermal conductivity of nanofluids
Buongiorno, Jacopo,Venerus, David C.,Prabhat, Naveen,McKrell, Thomas,Townsend, Jessica,Christianson, Rebecca,Tolmachev, Yuriy V.,Keblinski, Pawel,Hu, Lin-wen,Alvarado, Jorge L.,Bang, In Cheol,Bishnoi, American Institute of Physics 2009 JOURNAL OF APPLIED PHYSICS - Vol.106 No.9
Rael, Leonard T.,Thomas, Gregory W.,Craun, Michael L.,Curtis, C. Gerald,Bar-Or, Raphael,Bar-Or, David 한국생화학분자생물학회 (구 한국생화학회) 2004 BMB Reports Vol.37 No.6
Saturated fatty acids are less vulnerable to lipid peroxidation than their unsaturated counterparts. In this investigation, individual fatty acids of the C_(16), C_(18) and C_(20) families were subjected to the thiobarbituric (TBA) assay. These fatty acids were chosen based on their degree of saturation and configuration of double bonds. Interestingly, an assay threshold was reached where increasing the fatty acid concentration resulted in no additional decrease in the TBARS concentrations. Therefore, the linear range of TBARS inhibition was determined for fatty acids in the C_(16) and C_(20) families. The rate of TBARS inhibition was greater for the saturated than for unsaturated fatty acids, as measured from the slope of the linear range. These findings demonstrate the need to standardize the TBARS assay using multiple fatty acid concentrations when using this assay for measuring in vitro lipid peroxidation.
Rael, Leonard T.,Thomas, Gregory W.,Craun, Michael L.,Curtis, C. Gerald,Bar-Or, Raphael,Bar-Or, David Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.6
Saturated fatty acids are less vulnerable to lipid peroxidation than their unsaturated counterparts. In this investigation, individual fatty acids of the $C_{16}$, $C_{18}$ and $C_{20}$ families were subjected to the thiobarbituric (TBA) assay. These fatty acids were chosen based on their degree of saturation and configuration of double bonds. Interestingly, an assay threshold was reached where increasing the fatty acid concentration resulted in no additional decrease in the TBARS concentrations. Therefore, the linear range of TBARS inhibition was determined for fatty acids in the $C_{16}$ and $C_{20}$ families. The rate of TBARS inhibition was greater for the saturated than for unsaturated fatty acids, as measured from the slope of the linear range. These findings demonstrate the need to standardize the TBARS assay using multiple fatty acid concentrations when using this assay for measuring in vitro lipid peroxidation.
Dual Antiplatelet Therapy Duration Based on Ischemic and Bleeding Risks After Coronary Stenting
Costa, Francesco,Van Klaveren, David,Feres, Fausto,James, Stefan,Rä,ber, Lorenz,Pilgrim, Thomas,Hong, Myeong-Ki,Kim, Hyo-Soo,Colombo, Antonio,Steg, Philippe Gabriel,Bhatt, Deepak L.,Stone, Gregg W Elsevier Inc. 2019 Journal of the American College of Cardiology Vol.73 No.7
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Complex percutaneous coronary intervention (PCI) is associated with higher ischemic risk, which can be mitigated by long-term dual antiplatelet therapy (DAPT). However, concomitant high bleeding risk (HBR) may be present, making it unclear whether short- or long-term DAPT should be prioritized.</P> <P><B>Objectives</B></P> <P>This study investigated the effects of ischemic (by PCI complexity) and bleeding (by PRECISE-DAPT [PREdicting bleeding Complications in patients undergoing stent Implantation and SubsequEnt Dual AntiPlatelet Therapy] score) risks on clinical outcomes and on the impact of DAPT duration after coronary stenting.</P> <P><B>Methods</B></P> <P>Complex PCI was defined as ≥3 stents implanted and/or ≥3 lesions treated, bifurcation stenting and/or stent length >60 mm, and/or chronic total occlusion revascularization. Ischemic and bleeding outcomes in high (≥25) or non-high (<25) PRECISE-DAPT strata were evaluated based on randomly allocated duration of DAPT.</P> <P><B>Results</B></P> <P>Among 14,963 patients from 8 randomized trials, 3,118 underwent complex PCI and experienced a higher rate of ischemic, but not bleeding, events. Long-term DAPT in non-HBR patients reduced ischemic events in both complex (absolute risk difference: −3.86%; 95% confidence interval: −7.71 to +0.06) and noncomplex PCI strata (absolute risk difference: −1.14%; 95% confidence interval: −2.26 to −0.02), but not among HBR patients, regardless of complex PCI features. The bleeding risk according to the Thrombolysis In Myocardial Infarction scale was increased by long-term DAPT only in HBR patients, regardless of PCI complexity.</P> <P><B>Conclusions</B></P> <P>Patients who underwent complex PCI had a higher risk of ischemic events, but benefitted from long-term DAPT only if HBR features were not present. These data suggested that when concordant, bleeding, more than ischemic risk, should inform decision-making on the duration of DAPT.</P> <P><B>Central Illustration</B></P> <P>[DISPLAY OMISSION]</P>
( Natalia Czerwonka ),( Puneet Gupta ),( Sohil S. Desai ),( Thomas R. Hickernell ),( Alexander L. Neuwirth ),( David P. Trofa ) 대한슬관절학회 2023 대한슬관절학회지 Vol.35 No.-
Background The purpose of this study is to provide a systematic review of the literature pertaining to Patient- Reported Outcome Measurement Information System (PROMIS) validation and utilization as an outcomes metric in total knee arthroplasty (TKA) patients. This is the first systematic review on PROMIS use in total knee arthroplasty patients. Methods A systematic search of the Pubmed/MEDLINE and Embase databases was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Study characteristics, patient demographics, psychometric properties (Pearson and Spearman correlation) with legacy patient-reported outcome measurement (PROM) instruments, floor and ceiling effects, responsiveness, and minimum clinically important difference (MCID) and PROMIS outcomes were recorded and analyzed. Results Fifteen studies investigating PROMIS in 11,140 patients were included. The weighted-average Pearson correlation coefficient comparing PROMIS domains with legacy patient-reported outcome measurements in total knee arthroplasty patients was 0.62 [standard error (SE) = 0.06] and the weighted-average Spearman correlation comparing PROMIS domains with legacy patient-reported outcome measurements in total knee arthroplasty patients was 0.59 (SE = 0.06), demonstrating moderate-to-strong correlation and validity. There were no differences in weighted average floor [0.03% (SE = 3.1) versus 0% (SE = 0.1) versus 0.01% (SE = 1.1); p = 0.25] or ceiling effects [0.01% (SE = 0.7) versus 0.02% (SE = 1.4) versus 0.04% (SE = 3.5); p = 0.36] between PROMIS and legacy instruments. The weighted average for percentage of patients achieving MCID was 59.1% for global physical health (GPH), 26.0% for global mental health (GMH), 52.7% for physical function (PF), 67.2% for pain interference (PI), and 37.2% for depression. Conclusion Notably, PROMIS global physical health, physical function, and pain interference were found to be significantly responsive, with PROMIS pain interference most effectively capturing clinical improvement as evidenced by the achievement of MCID.
Prospective F-18 FDOPA PET Imaging Study in Human PD
Vijay Dhawan,Martin H Niethammer,Martin L Lesser,Karalyn N Pappas,Matthew Hellman,Toni M Fitzpatrick,David Bjelke,Jaskirat Singh,Loreta M Quatarolo,Yoon Young Choi,Alice Oh,David Eidelberg,Thomas Chal 대한핵의학회 2022 핵의학 분자영상 Vol.56 No.3
Purpose We present the findings of our final prospective study submitted to the U.S. Food and Drug Administration (FDA) for New Drug Application (NDA) approval for the use of 3,4-dihydroxy-6-[18F]fluoro-L-phenylalanine (F-18 FDOPA) positron emission tomography (PET) imaging for Parkinson’s disease (PD). The primary aim was to determine the sensitivity, specificity, and predictive values of F-18 FDOPA PET in parkinsonian patients with respect to clinical standard-oftruth (SOT). Secondary outcomes included the inter-rater reliability, and correlation of quantitative measures for PET with dopaminergic status. Methods In 68 parkinsonian subjects, F-18 FDOPA PET scan from 80 to 100 min was acquired following a CT scan. Scan images were presented to one expert in F-18 FDOPA image interpretation and two physicians with prior experience in I-123 FPCIT single-photon emission computed tomography image interpretation. Fifty-six subjects completed the study with a followup for SOT determination. Image readers were blind to the clinical/quantitative data; SOT clinician was blind to the image data. Results For 47 of the 56 patients, SOT was in agreement with the PET scan results. For nine patients, SOT suggested dopaminergic deficit, whereas the imaging showed normal uptake. The specificity and positive predictive values are 91% and 92%, respectively, suggesting high probability that those who test positive by the PET scan truly have dopaminergic degeneration. The sensitivity was 73%. Inter-rater agreement was 0.6–0.8 between the different readers. Conclusion Our prospective study demonstrates high specificity and moderate sensitivity of F-18 FDOPA PET for PD. We received NDA approval in October 2019.