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- 저자 : Cuihong Li (검색결과 5 건)
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Xiaowei Yang,Cuihong Li,Hai Yu,Jinping Tang,Qinfang Wu,Wenjuan Qu 한국고분자학회 2023 Macromolecular Research Vol.31 No.8
Inflammatory bowel disease (IBD) is a kind of chronic inflammatory disease that is difficult to cure completely and may cause cancer. Modulating the intestinal flora is believed to be a feasible approach for IBD treatment. However, the traditional probiotics delivery systems often suffer from the inactivation caused by gastric acid. Herein, we proposed a novel probiotics-loaded microcapsule generated from a gas-assisted microfluidic platform. The microcapsules were composed of alginate shells and probiotics-containing cores, and exhibited good sphericity and biocompatibility, and had an average size of about 325 μm and a coefficient of variation of 2.57%. When the probiotics-loaded microcapsules were used for the IBD treatment of mice, they displayed good therapeutic effects in modulating oxidative stress and inflammation as well as protecting the intestinal barrier. These features indicate that the prepared probiotics-loaded microcapsules could be used as new materials for IBD treatment.
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Jianmin Gu,Baipeng Yin,Shaoyan Fu,Cuihong Jin,Xin Liu,Zhenpan Bian,Jianjun Li,Lu Wang,Xiaoyu Li 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.2
Due to the intense influence of the shape and size of the photon building blocks on the limitation and guidance of opticalwaves, an important strategy is the fabrication of different structures. Herein, organic semiconductor tris-(8-hydroxyquinoline)aluminium (Alq3) nanostructures with controllable morphology, ranging from one-dimensional nanowires to twodimensionalplates, have been prepared through altering intermolecular interactions with employing the anti-solvent diffusioncooperate with solvent-volatilization induced self-assembly method. The morphologies of the formed nanostructures, whichare closely related to the stacking modes of the molecules, can be exactly controlled by altering the polarity of anti-solventsthat can influence various intermolecular interactions. The synthesis strategy reported here can potentially be extended toother functional organic nanomaterials.
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Synthesis of S-adenosyl-L-methionine in Escherichia coli
Xiao-Nan Wei,Minjie Cao,Jian Li,Huan Li,Yi Song,Cuihong Du 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6
S-adenosyl-L-methionine (SAM) is an importantphysiological metabolite in vivo and may be useful inmedicines. SAM is produced from L-methionine and ATPcatalyzed by S-adenosyl-L-methionine synthetase (SAMS)in vivo. In this study, the gene encoding SAMS was clonedand a genetically engineered Escherichia coli (E. coli)BL21(pET-28a-SAMS) was constructed. The recombinantSAMS with a molecular mass of approximately 46 kDawas expressed by inducing the engineered E. coli usingisopropyl-β-D-1-thiogalactopyranoside (IPTG) as an inducer. To produce SAM using a low-cost, nontoxic and highperformanceexpression system, lactose was used as asubstitute for IPTG to induce BL21(pET-28a-SAMS). Byoptimizing the expression conditions, the concentration ofSAM produced by the engineered E. coli was 48 mg/L in theculture medium supernatant. To increase the concentrationof SAM produced, a coupled system was constructedconsisting of E. coli BL21(pET-28a-SAMS) and Saccharomycescerevisiae (S. cerevisiae) JM-310. In this coupled system,ATP generated from S. cerevisiae was provided to E. colifor producing a higher concentration of SAM. The SAMconcentration in the coupled system reached 1.7 g/L. SAMwas purified by a weak acid cationic exchange resin D113,and a simple and economical purification procedure forSAM isolation was achieved. SAM was confirmed byHigh Performance Liquid Chromatography-tandem MassSpectrometry analysis. Our study provides a feasible andconvenient approach to produce SAM.
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Rui Zhang,Lina Chen,Fei Huang,Xiaorong Wang,Cuihong Li 한국유전학회 2021 Genes & Genomics Vol.43 No.7
Background Many long non-coding RNAs (lncRNAs) have been suggested to play critical roles in acute lung injury (ALI) pathogenesis, including lncRNA nuclear enriched abundant transcript 1 (NEAT1). Objective We aimed to further elucidate the functions and molecular mechanism of NEAT1 in ALI. Methods Human pulmonary alveolar epithelial cells (HPAEpiCs) stimulated by lipopolysaccharide (LPS) were served as a cellular model of ALI. Cell viability and cell apoptosis were determined by cell counting kit-8 (CCK-8) assay and fow cytometry, respectively. The expression of NEAT1, microRNA-424-5p (miR-424-5p), and mitogen-activated protein kinase 14 (MAPK14) was measured by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot analysis. Caspase activity was determined by caspase activity kit. The infammatory responses were evaluated using enzyme-linked immunosorbent assay (ELISA). The oxidative stress factors were analyzed by corresponding kits. Results NEAT1 was upregulated in LPS-stimulated HPAEpiCs. NEAT1 knockdown weakened LPS-induced injury by inhibiting apoptosis, infammation and oxidative stress in HPAEpiCs. Moreover, miR-424-5p was a direct target of NEAT1, and its knockdown reversed the efects caused by NEAT1 knockdown in LPS-induced HPAEpiCs. Furthermore, MAPK14 was a downstream target of miR-424-5p, and its overexpression attenuated the efects of miR-424-5p on reduction of LPSinduced injury in HPAEpiCs. Besides, NEAT1 acted as a sponge of miR-424-5p to regulate MAPK14 expression. Conclusion NEAT1 knockdown alleviated LPS-induced injury of HPAEpiCs by regulating miR-424-5p/MAPK14 axis, which provided a potential therapeutic target for the treatment of ALI.
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