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        Assessment of Population Structure and Genetic Diversity of 15 Chinese Indigenous Chicken Breeds Using Microsatellite Markers

        Chen, Guohong,Bao, Wenbin,Shu, Jingting,Ji, Congliang,Wang, Minqiang,Eding, Herwin,Muchadeyi, Farai,Weigend, Steffen Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.3

        The genetic structure and diversity of 15 Chinese indigenous chicken breeds was investigated using 29 microsatellite markers. The total number of birds examined was 542, on average 36 birds per breed. A total of 277 alleles (mean number 9.55 alleles per locus, ranging from 2 to 25) was observed. All populations showed high levels of heterozygosity with the lowest estimate of 0.440 for the Gushi chickens, and the highest one of 0.644 observed for Wannan Three-yellow chickens. The global heterozygote deficit across all populations (FIT) amounted to 0.180 (p<0.001). About 16% of the total genetic variability originated from differences between breeds, with all loci contributing significantly to this differentiation. An unrooted consensus tree was constructed using the Neighbour-Joining method and pair-wise distances based on marker estimated kinships. Two main groups were found. The heavy-body type populations grouped together in one cluster while the light-body type populations formed the second cluster. The STRUCTURE software was used to assess genetic clustering of these chicken breeds. Similar to the phylogenetic analysis, the heavy-body type and light-body type populations separated first. Clustering analysis provided an accurate representation of the current genetic relations among the breeds. Remarkably similar breed rankings were obtained with all methods.

      • KCI등재

        Effects of Zn content on microstructure and magnetic properties of MnZn ferrite

        Yingming Zhang,Yujie Yang,Dongyang Chen,Congliang Chen,Yuting Meng 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2

        The Mn1-xZnxFe2O4 (x = 0.46, 0.47, 0.48, 0.50, 0.51) ferrite materials were prepared by solid-state reaction. The effects ofvarying Zn concentration on the magnetic characteristics and microstructure of MnZn ferrites were investigated. All of theseferrites are one spinel phase, according to XRD analysis, and no additional heterogeneous phases are generated. Thediffraction peaks of the samples appear to be shifted to a higher angle compared to the standard cards. The sample's very bigand irregular grain development caused the sample's grain size to vary as the zinc level increased. The power loss of MnZnferrite can be decreased because to this phenomena. The PCV value decreases as the Zn level of the samples increases. The PCVof this ferrite is as high as 242.6 mW/cm3 at a frequency of 150 kHz with a Zn concentration of x = 0.46. Under the same testcircumstances, ferrite's PCV value is only 115.2mW/cm3 when the Zn concentration is x = 0.48. The complex permeability ofthe samples rises with an increase in Zn concentration. In the ferrite sample with the highest Zn content (x = 0.51), its μ' valuekeeps at a high level with the change of frequency. When the frequency is 10 kHz, the μ' value is as high as 260.9. Additionally,all of these ferrite samples demonstrated stability against interference at frequencies lower than 150 kHz.

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        Risk factors for and delayed recognition of genitourinary fistula following radical hysterectomy for cervical cancer: a population-based analysis

        Cong Liang,Ping Liu,Shan Kang,Weili Li,BiLiang Chen,Mei Ji,Chunlin Chen 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.2

        Objective: This study aimed to identify the risk factors for genitourinary fistulas and delayed fistula recognition after radical hysterectomy for cervical cancer. Methods: This study was a retrospective analysis of data collected in the Major Surgical complications of Cervical Cancer in China (MSCCCC) database from 2004–2016. Data on sociodemographic characteristics, clinical characteristics, and hospital characteristics were extracted. Differences in the odds of genitourinary fistula development were investigated with multivariate logistic regression analyses, and differences in the time to recognition of genitourinary fistula were assessed by Kruskal–Wallis test. Results: In this study, 23,404 patients met the inclusion criteria. Surgery in a cancer center, a women’s and children’s hospital, a facility in a first-tier city, or southwest region, stage IIA, type C1 hysterectomy, laparoscopic surgery and ureteral injury were associated with a higher risk of ureterovaginal fistula (UVF) (p<0.050). Surgery in southwest region, bladder injury and laparoscopic surgery were associated with greater odds of vesicovaginal fistula (VVF) (p<0.050). Surgery at cancer centers and high-volume hospitals was associated with an increase in the median time to UVF recognition (p=0.016; p=0.005). International Federation of Gynecology and Obstetrics (FIGO) stage IIA1-IIB was associated with delayed recognition of VVF (p=0.040). Conclusion: Intraoperative urinary tract injury and surgical approach were associated with differences in the development of UVFs and VVFs. Patients who underwent surgery in cancer centers and high-volume hospitals were more likely to experience delayed recognition of UVF. Patients with FIGO stage IIA1-IIB disease were more likely to experience delayed recognition of VVF.

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