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Anna N. N. Hui,Maria L. W. Lee,Priscilla S. C. Yeung,Jannet H. Y. Chick,Adith K. Y. Ho,Carly K. Y. Ng 환태평양유아교육연구학회 2017 Asia-Pacific journal of research in early childhoo Vol.11 No.3
The current study examined the reliability and applicability of the Chinese translated versions of the Early Childhood Environment Rating Scale ?Revised (ECERS-R) and Early Childhood Environment Rating Scale ?Extension (ECERS-E) as formative tools for use by local practitioners to perform selfevaluations on the various aspects of the educational environments being provided to pre-school children in their own settings with the goal of helping to facilitate changes that may help to enhance the quality of educational experiences for young children. Five registered kindergartens were recruited and received trainings in the administration of the said tools by the PECERA-HK (ECERS) professional team. Three assessment trials were administered in three different phases. For each trial, teacher representatives from each kindergarten completed the assessment using the said tools together with the trainers. Discussions were also held with the trainers on their observations. Internal reliability for the tools was found to be quite robust. Qualitative findings also suggested that the ECERS-R and ECERS-E can serve to assist practitioners in identifying the respective strengths and weaknesses in the educational environment they were providing to children and in setting clear directions for making corresponding improvements.
Guang-Hong Chen,Chun-Ping Jen,Ching-Te Huang,Hsin-Hui Wu,Tatyana N. Zamay,Anna S. Zamay 한국바이오칩학회 2014 BioChip Journal Vol.8 No.2
Detecting rare cells, such as circulatingtumor cells (CTCs), circulating fetal cells, and stemcells, is vital during medical diagnostics and characterization. During carcinogenesis, cancer cells detachfrom the primary tumor into the blood stream, becomingCTCs. Typical rare cell samples are consideredany sample that contains less than 1000 target cellsper milliliter. The volumes of microfluidic devicestypically range from several microliters to nanoliters;this is excessively small for experimenting using lowconcentrationsamples. This study involved isolatingcancerous cells in an open-top chamber with sub-millilitervolumes (0.1 mL) of blood samples by using alysis buffer solution for red blood cells (RBCs), as wellas concentrating cells employing the dielectrophoreticforce generated using stepping electric fields,which were produced using a handheld electric modulethat comprised a voltage-frequency converterand an operational amplifier. To increase the samplevolume, an open-top chamber was fabricated on andbonded to a glass substrate by using circular microelectrodes. The concentrations of cancer cells andRBCs were adjusted to 500 cells/mL and 4×105 cell/mL, respectively, for the experiments. To reduce theinterference of blood cells during detection and isolateCTCs, the RBCs in the sample were lysed in alysis buffer solution before the proposed chip wasused to dielectrophoretically manipulate the rare cancerouscells. The findings indicated that the lysis bufferlysed the erythrocytes and the survivability levelsof the cancerous cells (HeLa and MCF-7) remainedhigh in the lysis buffer. The positive dielectrophoreticcancerous cells were guided based on the direction ofthe stepping electric field because of movement in thehigh-electric-field region; hence, the cancerous cellsconcentrated and collected at the central electrode.