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Callus Induction and Regeneration from Anther Cultures of Indonesian Indica Black Rice Cultivar
Anisa Maharani,Wahyu Indra Duwi Fanata,Faida Nur Laeli,김경민,Tri Handoyo 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.1
The assembly of superior varieties and collection of rice germplasm involves the process of selecting and storing elders that have superior genotypic properties and phenotypes. The anther culture techniques on indica black rice cultivar have a high difficulty factor to get plants, because of the low regeneration ability at the plant formation phase from the anther callus. This study aimed to investigate the influence of the cold-pretreatment time on anther, the combination of plant growth regulators (PGR’s) concentrations, and putrescine concentrations in media for the increase callus induction and plant regeneration of indica black rice. The optimization of the cold pre-treatment time was important to obtain the high-frequency callus induction, which showed that anther at the 4°C for 8 days formed the high callus induction (20%). To accelerate the callus induction, the application of 20 µM putrescine in the MS medium could produce more friable embryogenic callus for 24 days with 27% of callus formation. Generally, the optimal medium for the high frequency of callus induction contained 2 mgL-1 NAA+0.5 mgL-1 Kinetin+20 µM putrescine. Especially indica black rice cultivars, the best media to get a high plant regeneration frequency were N6 media containing the combination of 2 mgL-1 IAA and 2,5 mgL-1 Kinetin. The total callus regenerated to plantlet about 12.5%. The study of the callus induction and in-vitro plant regeneration medium for indica black rice were still important to develop to get the best result for other cultivars.
Cytotoxic Constituents from the Stem Bark of Chisocheton pentandrus
Rurini Retnowati,Hermin Sulistyarti,Nikmatus Zahro Wahidah,Anisa Lailatusy Syarifah,Suprianto Salam,Nurlelasari,Agus Safari,Desi Harneti,Mulyadi Tanjung,Ace Tatang Hidayat,Rani Maharani,Unang Supratma 한국생약학회 2021 Natural Product Sciences Vol.27 No.1
Eight cytotoxic constituents, consisting of six triterpenoids, cabralealactone (1), cabraleadiol (2), prototiamin A (3), 23-desmethyllimocin B (5), melianodiol (7) and indicalilacol (8) along with one limonoid, neemfruitins A (4) and one protolimonoid, protoxylocarpin G (6), were isolated from the extract of n-hexane of the stembark of Chisocheton pentandrus. The chemical structures were identified on the basis of spectroscopic evidence and compared to previously reported spectra. These isolated compounds appear for the first time in the plant. Compounds 1 - 8 were evaluated for their cytotoxic effect against MCF-7 breast cancer lines in vitro. Among the isolated compounds, melianodiol (7) showed the strongest cytotoxic activity with IC50 values of 16.8 mM.