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Nguyen Duc Anh,Pham Van Thanh,Doan Tu Lap,Nguyen Tuan Khai,Tran Van An,Tran Duc Tan,Nguyen Huu An,Dang Nhu Dinh 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.2
Forest fires inflict great losses of human lives and serious damages to ecological systems. Hence, numerous fire detection methods have been proposed, one of which is fire detection based on sensors. However, these methods reveal several limitations when applied in large spaces like forests such as high cost, high level of false alarm, limited battery capacity, and other problems. In this research, we propose a novel forest fire detection method based on image processing and correlation coefficient. Firstly, two fire detection conditions are applied in RGB color space to distinguish between fire pixels and the background. Secondly, the image is converted from RGB to YCbCr color space with two fire detection conditions being applied in this color space. Finally, the correlation coefficient is used to distinguish between fires and objects with fire-like colors. Our proposed algorithm is tested and evaluated on eleven fire and non-fire videos collected from the internet and achieves up to 95.87% and 97.89% of F-score and accuracy respectively in performance evaluation.
Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.
Anh Duc Truong,홍여진,이장근,이경백,Ha Thi Thanh Tran,Hoang Vu Dang,Viet Khong Nguyen,Hyun S. Lillehoj,홍영호 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.5
Objective: The inhibitory leukocyte immunoglobulin-like receptors (LILRBs) play an important role in innate immunity. The present study represents the first description of the cloning and structural and functional analysis of LILRB1 and LILRB3 isolated from two genetically disparate chicken lines. Methods: Chicken LILRB1-3 genes were identified by bioinformatics approach. Expression studies were performed by transfection, quantitative polymerase chain reaction. Signal transduction was analyzed by western blots, immunoprecipitation and flow cytometric. Cytokine levels were determined by enzyme-linked immunosorbent assay. Results: Amino acid homology and phylogenetic analyses showed that the homologies of LILRB1 and LILRB3 in the chicken line 6.3 to those proteins in the chicken line 7.2 ranged between 97%-99%, while homologies between chicken and mammal proteins ranged between 13%-19%, and 13%-69%, respectively. Our findings indicate that LILRB1 and LILRB3 subdivided into two groups based on the immunoreceptor tyrosine-based inhibitory motifs (ITIM) present in the transmembrane domain. Chicken line 6.3 has two ITIM motifs of the sequence LxYxxL and SxYxxV while line 7.2 has two ITIM motifs of the sequences LxYxxL and LxYxxV. These motifs bind to SHP-2 (protein tyrosine phosphatase, non-receptor type 11) that plays a regulatory role in immune functions. Moreover, our data indicate that LILRB1 and LILRB3 associated with and activated major histocompatibility complex (MHC) class I and β2-microglobulin and induced the expression of transporters associated with antigen processing, which are essential for MHC class I antigen presentation. This suggests that LILRB1 and LILRB3 are transcriptional regulators, modulating the expression of components in the MHC class I pathway and thereby regulating immune responses. Furthermore, LILRB1 and LILRB3 activated Janus kinase2/tyrosine kinase 2 (JAK2/TYK2); signal transducer and activator of transcription1/3 (STAT1/3), and suppressor of cytokine signaling 1 genes expressed in Macrophage (HD11) cells, which induced Th1, Th2, and Th17 cytokines. Conclusion: These data indicate that LILRB1 and LILRB3 are innate immune receptors associated with SHP-2, MHC class I, β2-microglobulin, and they activate the Janus kinase/signal transducer and activator of transcription signaling pathway. Thus, our study provides novel insights into the regulation of immunity and immunopathology.
EQCM studies of the effect of sulfamic acid on the formation Cu-Se compounds
Dang Thi Bich Hop,Pham Hong Quang,Duc H. Tran,Do Thi Kim Anh,Do Phuc Quan,Ngo Dinh Sang,W. B. K. Putri 한양대학교 세라믹연구소 2015 Journal of Ceramic Processing Research Vol.16 No.1
A combination of Cyclic Voltammetry (CV) and Electrochemical Quartz Crystal Microbalance (EQCM) has been used to study the effect of sulfamic acid as a complexing agent on the formation of Cu-Se compounds. The values of the equivalent atomic mass (M/z) grown at the gold EQCM sensor during electrodeposition at varying potentials and constant potentials were analyzed to understand the mechanism of the growing process. It was found that sulfamic acid does not affect so much the deposition of Cu in the absence of Se. However, when Cu and Se are present simultaneously, sulfamic acid causes and facilitates the formation of Cu-Se compounds. Furthermore, at a high concentration, sulfamic acid causes a mass-loss process, leading to a change of composition. A suitable concentration of sulfamic acid can be concerned from these studies.
Nonlinear dynamic response and vibration of nanocomposite multilayer organic solar cell
Duc, Nguyen Dinh,Seung-Eock, Kim,Quan, Tran Quoc,Long, Dang Dinh,Anh, Vu Minh Elsevier 2018 COMPOSITE STRUCTURES -BARKING THEN OXFORD- Vol.184 No.-
<P><B>Abstract</B></P> <P>In the recent years, organic solar cell (OSC) has attracted much interest of the research community due to its great promise as renewable sources. This paper presents the first analytical approach to investigate the nonlinear dynamic response and vibration of imperfect rectangular nanocompsite multilayer organic solar cell subjected to mechanical loads using the classical plate theory. Nanocompsite organic solar cell consists of five layers of Al, P3HT:PCBM, PEDOT:PSS, IOT and glass. Motion and compatibility equations are derived using the classical plate theory and taking into account the effects of initial geometrical imperfection and geometrical nonlinearity in Von Karman – Donnell sense. The Galerkin method and fourth – order Runge – Kutta method are used to give explicit expressions of natural frequencies, nonlinear frequency – amplitude relation and nonlinear dynamic responses of nanocompsite organic solar cell. The numerical results show the influences of geometrical parameters, the thickness of layers, imperfections, and mechanical loads on the nonlinear dynamic response and nonlinear vibration of nanocompsite organic solar cell.</P>
Truong, Anh Duc,Rengaraj, Deivendran,Hong, Yeojin,Tran, Ha Thi Thanh,Dang, Hoang Vu,Nguyen, Viet Khong,Lillehoj, Hyun S.,Hong, Yeong Ho MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.9
<P>The activating leukocyte immunoglobulin-like receptors (LILRAs) play an important role in innate immunity. However, most of the LILRA members have not been characterized in avian species including chickens. The present study is the first attempt at cloning, structural analysis and functional characterization of two LILRAs (LILRA2 and LILRA6) in chickens. Multiple sequence alignments and construction of a phylogenetic tree of chicken LILRA2 and LILRA6 with mammalian proteins revealed high conservation between chicken LILRA2 and LILRA6 and a close relationship between the chicken and mammalian proteins. The mRNA expression of <I>LILRA2</I> and <I>LILRA6</I> was high in chicken HD11 macrophages and the small intestine compared to that in several other tissues and cells tested. To examine the function of LILRA2 and LILRA6 in chicken immunity, <I>LILRA2</I> and <I>LILRA6</I> were transfected into HD11 cells. Our findings indicated that LILRA2 and LILRA6 are associated with the phosphorylation of Src kinases and SHP2, which play a regulatory role in immune functions. Moreover, LILRA6 associated with and activated MHC class I, β2-microglobulin and induced the expression of transporters associated with antigen processing but LILRA2 did not. Furthermore, both LILRA2 and LILRA6 activated JAK-STAT, NF-κB, PI3K/AKT and ERK1/2 MAPK signaling pathways and induced Th1-, Th2- and Th17-type cytokines and Toll-like receptors. Collectively, this study indicates that LILRA2 and LILRA6 are essential for macrophage-mediated immune responses and they have the potential to complement the innate and adaptive immune system against pathogens.</P>
Truong Anh Duc,Tran Ha Thi Thanh,Chu Nhu Thi,Nguyen Huyen Thi,부 티 하오,Hong Yeojin,송기덕,Dang Hoang Vu,홍영호 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4
Objective: Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens. Methods: Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq. Results: A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A-F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4-6, 9-10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and mitogen‑activated protein kinase (MAPK) proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. Kyoto encyclopedia of genes and genomes pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through quantitative real-time polymerase chain reaction and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R2 = 0.92– 0.95, p<0.01). Conclusion: This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.