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Ajeesh Chandrasekharan,이경원,김소담,이혜선,안선영,양승윤 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Three dimensional (3D)-bioprinting offers an innovative stratergy to fabricate functional tissues and organs with high complexicity and precision. Within this framework, digital light processing (DLP) technique using photocrosslinkable bioinks consisting of photosensitive biopolymers and photoinitiators (PIs) allows to prepare 3D complex hydrogels with tissue-specific properties. However, poor biocompatibility and low photoreactivity of the available bioinks limit their use in the fabrication of cell-laden 3D tissue constructs. Here, we report a fast-curable low-energy bioprinting system using a photosensitive hyaluronic acid (HA) and type I PIs based on benzoyl phosphinate for visible light-induced 3D bioprinting. After examining the water solubility, molar extinction coefficients, photocrosslinking efficiency and biocompatibility of the PIs, curved corneal structure was fabricated and the viability of corneal stroma cells in the 3D-printed constructs were evaluated. Overall, the HA-based photoreactive bioinks exhibited superior properties to function as a fast-curing visible light-induced 3D printing for use in tissue engineering applications.
Fast-Curable Tissue Adhesives for Corneal Wound Healing
이혜선,양승윤,Ajeesh Chandrasekharan,성금용,조연지,이종수 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
As a promising alternative of conventional sutures, glue-type tissue adhesives for wound closure is gaining popularity. However, difficulty in controlling adhesion time and poor wet adhesion of existing tissue adhesives limit their practical use in the clinic. Herein, we report in situ hydrogel-forming tissue glues based on photocrosslinkable hyaluronic acid (HA), providing firm tissue adhesion under wet and dynamic conditions. Depending on the degree of photocrosslinkable groups in HA polymer chains, mechanical properties and adhesive performance were improved. In the rabbit corneal incision model, the HA glue quickly sealed the incision site following low-energy UV exposure for 5 seconds and showed uniform corneal wound healing compared to control groups. Considering high biocompatibility and drug-loading capacity of HA hydrogels, the fast-curable HA glue can be applied for diverse biomedical applications, requiring long-term tissue adhesion and sustained drug delivery.
Hyaluronic Acid-based Hydrogel Microparticles for Enhanced Local Retention of Radioisotopes
김소담,임상구,Ajeesh Chandrasekharan,성금용,이태욱,김병연,김근영,최성용,양승윤 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
Radioisotopes provide diagnostic imaging and disease treatment for various diseases including cancer. In order to overcome the limitations of targeted radiation therapy using radioactive isotopes, long-term low-dose radiation therapy was performed by encapsulating the isotope in an injectable hydrogel. However, the difficulty of fabrication of radioactive hydrogels and unpredictable levels of radioactivity at the target site are major obstacles to clinical use. In this study, we developed the radioactive microgels using a <sup>131</sup>I-labeled photocrosslinkable hyaluronic acid (HA) and a high-throughput microdroplet generator. This approach allows rapid fabrication of injectable, radioactive, biodegradable (IRB) HA microgels, enabling immediate treatment with improved local retention. We demonstrated the clinical utility of this comprehensive approach by preparing IRB HA microgels within 15 minutes and localizing retention with minimal biodistribution beyond 2 weeks in target tissues in vivo.