YH1885의 체내동태(제1보) : 흰쥐에서 14C-YH1885의 단회투여시 흡수, 조직분포 및 배설

안병락(Byung Nak Ahn),Naoki Fujio,Naotoshi Kusumoto,Yoshifumi Abe,Masaaki Odomi,이종욱(Jong Wook Lee) 대한약학회 1997 약학회지 Vol.41 No.3

The absorption, distribution and excretion of 14C labeled YH1885 {5,6-Dimethyl-2(4-fluorophenylamino)-4-(1-methyl-1,2,3,4-tetrahydroisoquinolin-2-yl)pyrimidine hydrochloride), a new proton pumpinhibitor, were investigated in rats after a single administration of 14C-YH1885. 1. After intravenous administration of 5mg/kg, the blood level of radioactivity declined in a biphasic fashion with the mean terminal elimination half-life of 12.4hr. 2. After oral administration of 20mg/kg, the maximum blood level of radioactirity was reached at 4.0hr in female rats. The blood level of radioactivity-time profiles in male and female rats were similar, and the absorptionof 14C-YH1885 was not affected by food. 3. Appproximately 89% and 1% of radioactivity of the total dose were excreted in feces and urine, respectively. 4. Biliary excretion of radioactivity was 47.9% of the dose. Enterohepatic circulation of radioactivity was 49.6%. 5. Radioactivity was excreted maily into feces via bile. 6. The concentration of radioactivity in most tissues reached the peak level at 4.0hr after dosing, and then declined. Autoradiograms of male rats showed that the radioactivity levlels in the fat, harder's gland, liver and G-Itract were higher than those in the other tissues and the elimination of radioactivity from fat and liver was slow. 7. Autoradiograms of a pregnant rat showed that radioactivity was transferred to mammary gland, placenta and fetus. The radioactivity level in the mammary gland was higher than that in the blood.

응집제 및 응집보조제 투입방법에 따른 음식물폐수의 처리특성

성낙창 ( Nak-chang Sung ),한영립 ( Young-rip Han ),정병길 ( Byung-gil Jung ),안영희 ( Yeong-hee Ahn ),최영익 ( Young-ik Choi ) 한국환경기술학회 2014 한국환경기술학회지 Vol.15 No.4

본 연구는 음식물폐수 처리를 위해 응집공정의 효율 향상을 위한 방안으로 응집보조제를 투입하여 음식물폐수내 유기물처리 특성을 파악하고자 하였다. 연구를 위해 최적 응집제 투입 조건을 파악하기 위해 주입량, 교반조건 등을 Jar-test로 연구하였으며, 결과를 바탕으로 응집보조제 투입에 따른 유기물 농도를 파악하였다. 연구결과 최적응집제 투입량을 4.5 mL/L로 나타났으며, 최적응집제 투입량을 바탕으로 응집보조제를 투입하여 제거효율을 평가한 결과 COD_Cr, TOC, UV₂₅₄, 탁도 및 SS 항목에서 응집보조제 주입량 2.25 mL/L에서 가장 높은 효율을 나타내었다. 또한, 응집보조제 주입 방안에 관하여 응집 전주입, 응집 후 주입 및 동시주입으로 구분하여 실시한 결과 응집 전 주입 → 응집 후 주입 → 동시주입 순으로 나타났다. This study has improved coagulation processes in order to design a treatment plan by improving treatment process of food wastewater that is generated from a resource recovery plant of food wast. The optimal coagulation conditions were derived by observing the features of coagulation·mixing according to characteristics of water quality of food wastewater through a jar-tests. Based on them, optimal operating parameters were derived by analyzing treated wastewater quality. The optimal dosage of a polymer coagulant for food wastewater was found to be 4.5 mL/L. Besides, the optimal dosage of the polymer coagulant aids which is the optimal dosage of a polymer coagulant for food wastewater was found to be 2.25 mL/L in COD_Cr, TOC, UV₂₅₄, turbidity and SS. As a result of conducting the Jar-tests by changing the location of injection of the coagulant aids under optimal coagulation conditions into pre-injection, post-injection and co-injection in order to decide the location of injection of coagulant aids for food wastewater. They showed high removal efficiencies in the order of pre-injection → post-injection → co-injection.

디젤오염지역에서 분리한 세균 Sphingomonas sp. 3Y의 석유계 탄화수소분해특성

안영희(Yeonghee Ahn),정병길(Byung-Gil Jung),성낙창(Nak-Chang Sung),이영옥(Young-Ok Lee) 한국생명과학회 2009 생명과학회지 Vol.19 No.5

장기간 경유로 오염된 지역의 토양으로부터 분리한 세균 3Y는 석유계 탄화수소를 구성하는 다양한 화합물을 유일 탄소원으로하여 성장하였다. Sphingomonas sp. 3Y는 지방족 화합물은 물론이고 방향족 화합물을 이용해서 성장할 수 있었다. 지방족 화합물로서는 hexane과 hexadecane을 이용하여 성장하였고, 한편 방향족 화합물로서는 BTEX는 물론이고 phenol, biphenyl, 또는 phenanthrene을 유일 탄소원으로 이용하여 성장하였다. 본 균주는 indole과 catechol을 이용한 실험결과 방향족 탄화수소의 생분해 과정에서 맨 첫 단계 반응에 관여하는 효소인 aromatic ring dioxygenase 활성과 benzene 환을깨는 효소인 meta-cleavage dioxygenase 활성을 나타내었다. Sphingomonas sp. 3Y의 16S rRNA 유전자의 염기서열 분석과계통수 작성 결과 본 균주는 α-Proteobacteria인 Sphingomonas속에 해당하였으며 지금까지 잘 알려진 석유계 탄화수소를 분해하는 Sphingomonas sp. 균주들과는 다른 cluster를 형성하였다. 다양한 석유계 탄화수소 성분을 이용하여 성장하는 Sphingomonas sp. 3Y는 유류로 오염된 토양의 복원에 유용하게 사용될 것으로 여겨지며 이 균주의 최적 분해 조건을 조사한다면 그 결과는 이 균주가 분리된 오염지역의 생물학적 분해를 최적화하는데 기여할 것이다. Bacterial stain 3Y was isolated from a site that was contaminated with diesel for more than 15 years. The strain could grow on various petroleum using hydrocarbons as the sole carbon source. The strain grew not only on aliphatic hydrocarbons but also on aromatic hydrocarbons. 3Y grew on aliphatic petroleum hydrocarbons hexane or hexadecane, and aromatic petroleum hydrocarbons BTEX, phenol, biphenyl, or phenanthrene. The strain showed aromatic ring dioxygenase and meta-cleavage dioxygenase activities as determined by tests using indole and catechol. Aromatic ring dioxygenase is involved in the initial step of biodegradation of aromatic hydrocarbons while meta-cleavage dioxygenase catalyzes the cleavage of the benzene ring. Based on a nucleotide sequence analysis of its 16S rRNA gene, 3Y belongs to the genus Sphingomonas. A phylogenetic tress was constructed based on the nucleotide sequences of closest relatives of 3Y and petroleum hydrocarbon degrading sphingomonads. 3Y was in a cluster that was different from the cluster that contained well-known sphingomonads. The results of this study suggest that 3Y has the potential to cleanup oil-contaminated sites. Further investigation is warranted to optimize conditions to degrade petroleum hydrocarbons by the strain to develop a better bioremediation strategy.

ISSR 표지자를 이용한 느릅나무 자연집단의 유전변이 분석

안지영 ( Ji Young Ahn ),홍경낙 ( Kyung Nak Hong ),이제완 ( Jei Wan Lee ),양병훈 ( Byung Hoon Yang ) 한국산림과학회 2013 한국산림과학회지 Vol.102 No.4

Population genetic structure and diversity of Ulmus davidiana var. japonica in South Korea were studied using ISSR markers. A total of 45 polymorphic ISSR amplicons were cropped from 7 ISSR primers and 171 individuals of 7 populations. The average of effective alleles and the proportion of polymorphic loci were 1.5 and 89% respectively. The Shannon`s diversity index (I) was 0.435 and the expected heterozygosity from the frequentist`s method (He) and the Bayesian inference (hs) were 0.289 and 0.323 respectively. From AMOVA, 4.2% of total genetic variation in the elm populations was explained with the difference among populations (Φ ST=0.042) and the other 95.8% was distributed within populations. The θII value by Bayesian method which was comparable to the FST was 0.043. So the level of genetic diversity in the elm populations was similar to that in Genus Ulmus and the level of genetic differentiation was lower than that of others. No population showed a significant difference in the population-specific fixation indices (average of PS-FIS=0.822) or the population-specific genetic differentiations (average of PS-FST=0.101). Seven populations were allocated into 3 groups in the UPGMA and the PCA, but the grouping patterns were different. Also, we could not confirm any geographic trend from Bayesian clustering.

Core–shell strain structure of zeolite microcrystals

Cha, Wonsuk,Jeong, Nak Cheon,Song, Sanghoon,Park, Hyun-jun,Thanh Pham, Tung Cao,Harder, Ross,Lim, Bobae,Xiong, Gang,Ahn, Docheon,McNulty, Ian,Kim, Jungho,Yoon, Kyung Byung,Robinson, Ian K.,Kim, Hyunju Nature Publishing Group 2013 Nature materials Vol.12 No.8

Zeolites are crystalline aluminosilicate minerals featuring a network of 0.3–1.5-nm-wide pores, used in industry as catalysts for hydrocarbon interconversion, ion exchangers, molecular sieves and adsorbents. For improved applications, it is highly useful to study the distribution of internal local strains because they sensitively affect the rates of adsorption and diffusion of guest molecules within zeolites. Here, we report the observation of an unusual triangular deformation field distribution in ZSM-5 zeolites by coherent X-ray diffraction imaging, showing the presence of a strain within the crystal arising from the heterogeneous core–shell structure, which is supported by finite element model calculation and confirmed by fluorescence measurement. The shell is composed of H-ZSM-5 with intrinsic negative thermal expansion whereas the core exhibits a different thermal expansion behaviour due to the presence of organic template residues, which usually remain when the starting materials are insufficiently calcined. Engineering such strain effects could have a major impact on the design of future catalysts.

Effect of Food on the Pharmacokinetics of YH439 and Its Metabolites in Rats

Kim, Moon-Kyoung,Ahn, Byung-Nak,Yoo, Joong-Keun,Lee, Jong-Wook The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.6

The pharmacokinetics of YH439 and its metabolites were investigated after oral administration of YH439 to rats to investigate the food effect. After oral administration of YH439, its metabolites, M4 and M5 were detected in plasma. YH439 was not detected in the plasma for both fasted and fed rats for all doses studied. The pharmacokinetic parameters of the metabolites were not affected by food at all doses studied. The results of this study indicated that there are no significant food effects on the pharmacokinetics of YH439 and its metabolites in rats.

Effect of Gamma irradiation on the Protein Contents of Seafood Cooking Drips

Hyun-Joo Kim,Jong-il Choi,Nak-Yun Sung,Eui-Baek Byun,Jae-Hun Kim,Byung-Soo Chun,Dong-Hyun Ahn,Myung-Woo Byun,Ju-Woon Lee 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.4

Expression of active Recombinant Human Cytochrome P450 3A4 for In Vitro Drug Metabolism Study

Keum Sehoon,Cha Myung Hun,Shim Jaeyoung,Ahn Byung-Nak,Kang Heui ll 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.1

감마선 조사에 의한 수산 자숙액의 단백질 함량 변화

최종일(Jong-il Choi),김현주(Hyun-Joo Kim),성낙윤(Nak-Yun Sung),변의백(Eui-Baek Byun),김재훈(Jae-Hun Kim),전병수(Byung-Soo Chun),안동현(Dong-Hyun Ahn),조국연(Kook Yeon Cho),변명우(Myung-Woo Byun),이주운(Ju-Woon Lee) 한국생물공학회 2008 KSBB Journal Vol.23 No.6

방사선 조사에 의한 문어 자숙액의 항산화 활성 증가 원인 규명

최종일(Jong-il Choi),김연주(Yeon-Joo Kim),성낙윤(Nak-Yun Sung),김재훈(Jae-Hun Kim),안동현(Dong-Huyn Ahn),전병수(Byung-Soo Chun),조국연(Kook Yeon Cho),변명우(Myung-Woo Byun),이주운(Ju-Woon Lee) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.1

문어 자숙액 에탄올 추출물의 감마선 및 전자선 조사에 의한 항산화능의 증가 원인을 규명하고자 본 실험을 실시하였다. 문어 자숙액 에탄올 추출물에 polyclar™를 처리하여 폴리페놀을 흡착시켜 제거한 자숙액 추출물의 방사선 조사에 따른 DPPH 라디칼 소거능 변화를 측정한 결과, 폴리페놀이 제거되지 않은 자숙액에서와 같은 방사선 조사에 의한 항산화능 증가가 확인되어졌다. 따라서 방사선 조사에 의한 자숙액의 항산화능의 증가는 폴리페놀에 의한 원인이 아닌것으로 판단되었다. 자숙액에 많은 함량을 차지하고 있는 단백질에 대한 방사선 영향을 확인하기 위하여 (NH4)2SO4를 이용한 침전법으로 분리하여 얻어진 문어 자숙액 단백질 수용액에 방사선 조사한 결과 감마선 및 전자선 조사에 의하여 50% 이상 라디칼 소거능이 증가하였다. 이러한 연구결과는 문어 자숙액에 감마선 및 전자선 조사를 적용하였을 때 항산화 활성이 증가하는 이유는 폴리페놀의 함량의 증가에 의한 것보다는 조사에 의해 단백질 구조가 변화되고 이에 따라 항산화 활성이 증가하게 되는 것이라고 사료된다. This study was conducted to investigate the reason for the increase in the antioxidant activity of cooking drip from Enteroctopus dofleini by gamma and electron-beam irradiation. To define the effect of irradiation on the antioxidant activity of polyphenol in cooking drip, polyphenol excluded cooking drip extracts were prepared with polyclar™. But, the antioxidant activity of the cooking drip extracts without polyphenol was still increased by both irradiations. Instead, the effect on the proteins in the cooking drips prepared by ammonium sulfate precipitation showed great increase in antioxidative activity by irradiation. From these results, it was concluded that the increase in the antioxidative activity in cooking drips by the irradiation was caused by the structural modification of proteins in the cooking drips.