Wound Healing Activity of the Chloroform Extract of Plumbago rosea Linn. and Plumbagin

A. Saraswathy,R.V. Pradeep Chandran,B. Murali Manohar,S.Vairamuthu 한국생약학회 2006 Natural Product Sciences Vol.12 No.1

wound healing activity of plumbagin and the chloroform extract of Plumbago rosea Linn. (Yoot),incorporated into ointments with yellow soft paraffin, have been investigated on rats. Wound healing activity wasstudied using excision and incision wound models in rats following topical application. Both plumbagin and thePlumbago rosea root extract produced a significant response in both of the wound models studied. The woundPlumbago rosea root extract (0.5%),as against in 22 days in the case of control animals. The results were also comparable to those of a standard drug,framycetin sulphate cream (1% w/w) in terms of wound contracting ability, wound closure time, tensile strengthof wound and regeneration of tissues at the wound site. Histological studies revealed evidences for the healingprocess by formation of fibrovascular tissue, epithelization and increased collagenization when compared tocontrol.Keywordswound healing activity, Plumbago rosea, plumbagin, Plumbaginaceae

Identification of Novel Alternatively Spliced Transcripts of RBMS3 in Skeletal Muscle with Correlations to Insulin Action in vivo

Yong-Ho Lee,Stephen Tokraks,Saraswathy Nair,Clifton Bogardus,Paska A. Permana 대한의생명과학회 2009 Biomedical Science Letters Vol.15 No.4

Whole-body insulin resistance results largely from impaired insulin-stimulated glucose disposal in skeletal muscle. Our previous studies using differential display and quantitative real-time RT-PCR have shown that a novel cDNA band (DD23) had a higher level of expression in insulin resistant skeletal muscle and it was correlated with whole-body insulin action, independent of age, sex, and percent body fat. In this study, we cloned and characterized DD23. The DD23 sequence is part of the 3' UTR region of the RNA binding motif, single stranded interacting protein (RBMS3). We have cloned the full length cDNA for RBMS3 and identified two splice variants. These variants named DD23-L and DD23-S have 15 and 14 exons respectively and differ from RBMS3 in the 3' UTR significantly. Northern blot analyses showed that an ~8.8 kb mRNA transcript of DD23 was predominantly expressed in skeletal muscle and to a lesser extent in placenta, but not in heart, brain, lung, liver, or kidney, unlike RBMS3. Elevated expression levels of these novel alternatively spliced variants of RBMS3 in skeletal muscle may play a role in whole body insulin resistance.

Dysregulation of Cannabinoid CB1 Receptor Expression in Subcutaneous Adipocytes of Obese Individuals

이용호,William G. Tharp,Anne E. Dixon,Laurie Spaulding,Susanne Trost,Saraswathy Nair,Paska A. Permana,Richard E. Pratley 한국통합생물학회 2009 Animal cells and systems Vol.13 No.4

The endocannabinoid system (ECS) plays a key role in the regulation of appetite, body weight and metabolism. We undertook the present study to further clarify the regulation of the cannabinoid CB1 receptor (CB1, CNR1) in human adipose tissue in obesity. CB1 receptor mRNA expression was ~1.6-fold (P<0.004) and 1.9-fold higher (P<0.05) in subcutaneous adipocytes from obese compared to non-obese subjects in microarray and quantitative real-time PCR studies, respectively. Higher CB1 receptor mRNA expression levels in both adipose tissue (~1.2 fold, P<0.05) and adipocytes (~2 fold, P<0.01) were observed in samples from visceral compared to subcutaneous depots collected from 22 obese individuals. Immunofluorescence confocal microscopy demonstrated the presence of CB1 receptor on adipocytes and also adipose tissue macrophages. These data indicate that adipocyte CB1 receptor is up-regulated in human obesity and visceral adipose tissue and also suggest a potential role for the ECS in modulating immune/inflammation as well as fat metabolism in adipose tissue. The endocannabinoid system (ECS) plays a key role in the regulation of appetite, body weight and metabolism. We undertook the present study to further clarify the regulation of the cannabinoid CB1 receptor (CB1, CNR1) in human adipose tissue in obesity. CB1 receptor mRNA expression was ~1.6-fold (P<0.004) and 1.9-fold higher (P<0.05) in subcutaneous adipocytes from obese compared to non-obese subjects in microarray and quantitative real-time PCR studies, respectively. Higher CB1 receptor mRNA expression levels in both adipose tissue (~1.2 fold, P<0.05) and adipocytes (~2 fold, P<0.01) were observed in samples from visceral compared to subcutaneous depots collected from 22 obese individuals. Immunofluorescence confocal microscopy demonstrated the presence of CB1 receptor on adipocytes and also adipose tissue macrophages. These data indicate that adipocyte CB1 receptor is up-regulated in human obesity and visceral adipose tissue and also suggest a potential role for the ECS in modulating immune/inflammation as well as fat metabolism in adipose tissue.

Evaluation of Composite Cements using Cyclic Polarization Techniques

V. Saraswathy,S. Muralidharan,L. Balamurugan,P. Kathirvel,A. S. S. Sekar 대한토목학회 2011 KSCE JOURNAL OF CIVIL ENGINEERING Vol.15 No.8

Slag Cement (PSC) extracts in the presence and absence of nitrite inhibitor were studied by cyclic polarization technique. Studies also carried out in composite (binary and ternary) cement extracts. The re-passivation potential (Erep) is an important parameter to characterize the corrosion resistant property of cements and the efficiency of inhibitor. Blended cements (PPC and PSC) have higher tolerable limit of chloride than OPC. Binary cements increase the tolerable limit of chloride than individual cements. The passivity of steel maintained by ternary cement was found to be less than 45000 ppm of chloride along with 5000 ppm of nitrite (pitting occurred at 45000 ppm). This chloride level is more than that of chlorides in natural sea water (35000 ppm). Hence the use of composite cement especially in marine atmosphere increases the corrosion resistance of steel in concrete.

Self-Compacting Concrete Using Marble Sludge Powder and Crushed Rock Dust

M. Shahul Hameed,A. S. S. Sekar,L. Balamurugan,V. Saraswathy 대한토목학회 2012 KSCE JOURNAL OF CIVIL ENGINEERING Vol.16 No.6

Self Compacting Concrete (SCC) has had a remarkable impact on the concrete construction industry, especially the precast concrete industry. Crushed Rock Dust (CRD) and Marble Sludge Powder (MSP) are discarded in the nearby land and the natural fertility of the soil is spoiled. MSP and CRD can be used as filler and helps to reduce the total voids content in concrete. Consequently, this contributes to improve the strength of concrete. An experimental investigation has been carried out to study the combined effect of addition of MSP and CRD on the strength and durability of SCC. The study on physical, chemical and mechanical properties such as compressive strength and split tensile strength and the durability tests include water absorption test, water permeability, rapid chloride permeability; electrical resistivity and half cell potential are carried out in this study. From the results it is confirmed that compressive strength increases with increase in percentage replacement of MSP up to 15% of CRD in place of FA. It is found that split tensile strength is directly proportional to the compressive strength. The highest electrical resistivity values were obtained for Normal Concrete with 100% CRD and significant increase in resistivity values for SCC. Self Compacting Concrete (SCC) has had a remarkable impact on the concrete construction industry, especially the precast concrete industry. Crushed Rock Dust (CRD) and Marble Sludge Powder (MSP) are discarded in the nearby land and the natural fertility of the soil is spoiled. MSP and CRD can be used as filler and helps to reduce the total voids content in concrete. Consequently, this contributes to improve the strength of concrete. An experimental investigation has been carried out to study the combined effect of addition of MSP and CRD on the strength and durability of SCC. The study on physical, chemical and mechanical properties such as compressive strength and split tensile strength and the durability tests include water absorption test, water permeability, rapid chloride permeability; electrical resistivity and half cell potential are carried out in this study. From the results it is confirmed that compressive strength increases with increase in percentage replacement of MSP up to 15% of CRD in place of FA. It is found that split tensile strength is directly proportional to the compressive strength. The highest electrical resistivity values were obtained for Normal Concrete with 100% CRD and significant increase in resistivity values for SCC.

Toxicity Studies on Peristrophe paniculata (Forssk) Brummitt-an Ayurveda Drug.

R.V. Pradeep Chandran,A. Saraswathy,B. Murali Manohar,S. Vairamuthu 한국생약학회 2008 Natural Product Sciences Vol.14 No.2

Chronic oral toxicity studies (90 days) on aqueous and methanol extracts of the whole plant of Peristrophe paniculata (Forssk) Brummitt were carried out in Wistar rats. The dosage was 200 mg/kg/day, p.o. for both the extracts. All external morphological and biochemical changes, in addition to body weight and vital organ weights were recorded. During this investigation, no significant mortality was observed. The results showed that both the extracts were devoid of any toxicity at the dose level studied as compared to the control group.

Dysregulation of Cannabinoid CB1 Receptor Expression in Subcutaneous Adipocytes of Obese Individuals

Lee, Yong-Ho,Tharp, William G.,Dixon, Anne E.,Spaulding, Laurie,Trost, Susanne,Nair, Saraswathy,Permana, Paska A.,Pratley, Ridhard E. The Korean Society for Integrative Biology 2009 Animal cells and systems Vol.13 No.4

The endocannabinoid system (ECS) plays a key role in the regulation of appetite, body weight and metabolism. We undertook the present study to further clarify the regulation of the cannabinoid CB1 receptor (CB1, CNR1) in human adipose tissue in obesity. CB1 receptor mRNA expression was ~1.6-fold (p<0.004) and 1.9-fold higher (P<0.05) in subcutaneous adipocytes from obese compared to non-obese subjects in microarray and quantitative real-time PCR studies, respectively. Higher CB1 receptor mRNA expression levels in both adipose tissue (~1.2 fold, P<0.05) and adipocytes (~2 fold, P<0.01) were observed in samples from visceral compared to subcutaneous depots collected from 22 obese individuals. Immunofluorescence confocal microscopy demonstrated the presence of CB1 receptor on adipocytes and also adipose tissue macrophages. These data indicate that adipocyte CB1 receptor is up-regulated in human obesity and visceral adipose tissue and also suggest a potential role for the ECS in modulating immune/inflammation as well as fat metabolism in adipose tissue.