Reduction of Hydrogen Sulphide in Chicken Manure by Immobilized Sulphur Oxidising Bacteria Isolated from Hot Spring

M. Y. Hidayat1,H. M. Saud,A. A. Samsudin 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.1

The rapid development of the poultry industry has led to the production of large amounts of manure, which produce substances like hydrogen sulfide (H2S) that contribute to odor pollution. H2S is a highly undesirable gas component and its removal from the environment is therefore necessary. Sulfur-oxidizing bacteria (SOB) are widely known to remove contaminating H2S due to their ability to oxidize reduced sulfur compounds. In this study, three potential SOB (designated AH18, AH25, and AH28) that were previously isolated from a hot spring in Malaysia were identified by 16S rRNA gene analysis. Laboratory-scale biological deodorization experiments were conducted to test the performance of the three isolates—in the form of pure or mixed cultures, with the cells immobilized onto alginate as a carrier—in reducing the H2S from chicken manure. On the basis of 16S rRNA phylogenetic analysis, isolate AH18 was identified as Pseudomonas sp., whereas isolates AH25 and AH28 were identified as Achromobacter sp. The most active deodorizing isolate was AH18, with an H2S reduction rate of 74.7% (p < 0.05). Meanwhile, the reduction rates for isolates AH25 and AH28 were 54.2% and 60.8% (p > 0.05), respectively. However, the H2S removal performance was enhanced in the mixed culture, with a reduction rate of 81.9% (p < 0.05). In conclusion, the three potential SOB isolates were capable of reducing the H2S from chicken manure in the form of a pure culture immobilized on alginate, and the reduction performance was enhanced in the mixed culture.

Reduction of Hydrogen Sulphide in Chicken Manure by Immobilized Sulphur Oxidising Bacteria Isolated from Hot Spring

Hidayat, M.Y.,Saud, H.M.,Samsudin, A.A. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.1

The rapid development of the poultry industry has led to the production of large amounts of manure, which produce substances like hydrogen sulfide ($H_2S$) that contribute to odor pollution. $H_2S$ is a highly undesirable gas component and its removal from the environment is therefore necessary. Sulfur-oxidizing bacteria (SOB) are widely known to remove contaminating $H_2S$ due to their ability to oxidize reduced sulfur compounds. In this study, three potential SOB (designated AH18, AH25, and AH28) that were previously isolated from a hot spring in Malaysia were identified by 16S rRNA gene analysis. Laboratory-scale biological deodorization experiments were conducted to test the performance of the three isolates-in the form of pure or mixed cultures, with the cells immobilized onto alginate as a carrier-in reducing the $H_2S$ from chicken manure. On the basis of 16S rRNA phylogenetic analysis, isolate AH18 was identified as Pseudomonas sp., whereas isolates AH25 and AH28 were identified as Achromobacter sp. The most active deodorizing isolate was AH18, with an $H_2S$ reduction rate of 74.7% (p < 0.05). Meanwhile, the reduction rates for isolates AH25 and AH28 were 54.2% and 60.8% (p > 0.05), respectively. However, the $H_2S$ removal performance was enhanced in the mixed culture, with a reduction rate of 81.9% (p < 0.05). In conclusion, the three potential SOB isolates were capable of reducing the $H_2S$ from chicken manure in the form of a pure culture immobilized on alginate, and the reduction performance was enhanced in the mixed culture.

Preparation of Superparamagnetic Fe₃O₄ Nanoparticles from Iron Sand Mediated by Soft Template and Their Performance as Antibacterial Agent

A. Taufiq,A. F. Muyasaroh,S. Sunaryono,H. Susanto,N. Hidayat,N. Mufti,E. Suarsini,A. Hidayat,A. Okazawa,T. Ishida,D. Darminto 한국자기학회 2018 Journal of Magnetics Vol.23 No.3

In this work, the soft-template technique was employed in preparing the superparamagnetic Fe₃O₄ nanoparticles from natural iron sand. A series of the Fe3O4 nanoparticles formed spinel crystal structure with the particle size in the range of 1.9 to 6.6 nm which was varied by diethylamine concentration as the template. All samples had the functional groups of Fe<SUP>3+</SUP>-O<SUP>2−</SUP>, Fe<SUP>2+</SUP>-O<SUP>2−</SUP> and OH and exhibited the superparamagnetic character. The antibacterial activity of the Fe₃O₄ nanoparticles showed a significant outcome to pathogen growth rate. Pre-administration of bacterial stock solution (E. coli and B. substilis) with magnetite significantly reduced the colony formation compared to control group. In particular, for Gram-negative bacteria growth rate, pretreatment with magnetite declined the colony formation considerably compared to placebo and positive control group. Also, in line with Gram-negative bacteria, the similar pattern of the bacterial killing property was observed in Gram-positive bacteria.

Hydrothermal assisted synthesis of titanium dioxide nanoparticles modified graphene with enhanced photocatalytic performance

Rahmat Hidayat,Rahmat Hidayat,Tawfik A. Saleh 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

Water purification is a global issue, and most traditional methods have several drawbacks. As a result,new methods and materials must be devised to address the problem of water filtration. The hydrophobicTiO2-ZnO/rGO has been synthesized via single-step hydrothermal methods for the efficiency and effectiveindigo carmine (IC) solution degradation. The characterization of hydrophobic TiO2-ZnO/rGO has shownthat the material has a high contact angle value of around 103.6. Several photocatalytic parameters, pHof IC solution, the dosage of catalyst, and light intensity, were optimized for obtaining the highest degradationefficiency. The results of the photocatalytic study showed that the IC dye degradation process wascontrolled by superoxide anion radical (O2 radicals) to produce more specific products. Modificationswith hydrophobic compounds such as hexamethyldisilizane (HDMS) showed that they could increasethe photocatalytic properties and reusability of the material with a degradation percentage value of upto 95% with a constant rate of 0.0361 min1. Based on these results, this material has the potential tobe further developed for micro and complex photodegradation and self-cleaning materials.

Poster Session : PS 0138 ; Neurology : Transcranial Magnetic Stimulation (TMS) Improve Motoric Function in Post-Ischemic Stroke Patients, Indonesia Army Central Hospital Gatot Soebroto (RSPAD)

( Tugas Ratmono ),( Terawan A Putranto ),( Andri Hidayat ),( Bayu Putera ),( Joko Widodo ),( Lucia Hermiwati ),( Mulyana Mulyana ),( Gatot S Lawrence Lao ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Indonesian Basic Health Research Study (RISKESDAS) indicate that prevalence of stroke is 12.1%; and is considered as one of the major cause of death in Indonesia. It has been reported that ischemic stroke shown about 80-85% of the total cases, that continues to be the leading cause of long term disability worldwide. Primarily due to a loss of motor abilities and subsequent impairment in activities of daily living. Transcranial magnetic stimulation (TMS) is a non invasive tool that has been used in addition to other existing diagnostic tools to investigate motor pathophysiology in stroke patients. It has been shown to quantify the corticomotor excitability properties of clinically affected and unaffected muscles. Therefore, the objective of the study is to determine the usefulness of the device to rehabilitate the motoric function of the post-ischemic stroke patients Methods: March 2013 - March 2014, there has been 4380 cases of ischemic stroke, and only 2146 (48.9%) undergone intra-arterial heparin fl ushing guided with digital subtracted angiography (3D-DSA). Prior and post heparin-fl ushing procedure, all patients are undergone functional MRI test and assessment of the motoric function using TMS to examine the resting motor threshold (RMT), the amplitude (A), the latency (L), as well as Central Motoric conduction Time (CMCT) at the lesion and non lesion areas. Results: 2146 ischemic stroke patients, the RMT are 55.60±11.75 to 54.40±8.93 (p=0.52), and 52.78±10.59 to 52.22±8.59 (p=0.794); Amplitude are 1.57±1.76 to 1.7±1.72 (p=0.44), and 1.21±1.36 to 1.8±1.67 (p=0.005); Latency 23.76±4.51 to 23.51±4.36 (p=0.555) and 22.85±3.37 to 22.45±3.95 (p=0.104); and RMT 9.69±3.36 to 9.20±3.78 (p=0.238) and 8.87±2.92 to 8.02±2.58 (p=0.002) at the non lesion and lesion areas respectively. Conclusions: TMS provide a convincing evidence as new modality for post stroke rehabilitation.

ON THE TRIANGULAR EQUILIBRIUM POINTS IN THE ELLIPTIC RESTRICTED THREE-BODY PROBLEM UNDER RADIATION AND OBLATENESS EFFECTS

DERMAWAN, B.,HUDA, I.N.,WIBOWO, R.W.,HIDAYAT, T.,UTAMA, J.A.,MANDEY, D.,TAMPUBOLON, I. The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

This work considers the elliptic restricted three-body problem under effects of radiation of the bigger primary, and an oblate spheroid for the smaller primary to mimic an exoplanetary system with a gas giant planet. Under the influences of both effects we look for the existence of the triangular equilibrium points and the influences of the radiation and oblateness on the locations and motion of the points. We set the system in a normalized rotating coordinate system and derive equations of motion for the third infinitesimal object. Our study shows that the effects modify the equilateral/isosceles triangle shape with respect to the primaries. The triangular points also have non-planar motion with period depending on the value of the planet oblateness.

Indigenous materials revolution: Synthesizing porcelain alumina ceramic insulators for sustainable electrical systems in Indonesia

W. Garinas,A. Fauzi,H. Hidayaturrahman,Q.N. Madra,Subari,D.M. Nurjaman,W. Hidayat,Y. Sudiyanto,N.V. Permatasari,Zulfahmi,Yurohman 한양대학교 청정에너지연구소 2024 Journal of Ceramic Processing Research Vol.25 No.2

The modern electrical industry in Indonesia faces escalating energy demands, projected to reach 1,413 Terawatt Hours by2060. Meeting these demands necessitates enhancing the quality of electrical transmission systems, particularly insulators, witha focus on local materials to reduce dependency on imports. Porcelain Alumina Ceramic Insulators (PACI) offer promisingalternatives due to their high resistivity and mechanical strength. This study investigates PACI synthesis using locally sourcedmaterials: kaolin, ball clay, alumina, and feldspar. Chemical analysis confirms alumina as the primary component (99.7%Al2O3), while other materials contribute to silica content. Fabrication involves crushing, drying, grinding, sieving, and sinteringat 1280 °C, yielding specimens (K1S, K2S, K3S). Material characterization employs XRD, XRF, SEM and electrical testsreveals phase compositions, morphological changes, and electrical properties. K3S, rich in feldspar, exhibits superior materialstrength (74.62 N/mm²) and hardness (190 N/mm²) with limited conductivity. Utilizing local resources for PACI productionpromises economic benefits and reduces import dependence. This study illuminates the interplay between material compositionand resulting properties, offering insights into ceramic synthesis for improved electrical infrastructure.