Moraxella sp. CK-1 의 세포외 효소에 의한 Anabaena cylindrica 세포벽의 분해

김철호,최영길,민병례 ( Chul Ho Kim,Yong Keel Choi,Byung Re Min ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.1

Moraxella sp. CK-1 has been known to inhibit the growth of cyanobacteria including A. cylindrica. This growth inhibition by the bacteria was accomplished through the secretion of lytic enzyme (s) out of the bacterial cells. Extracellular cell wall lytic and proteolytic activity, which began to appear at the early exponential growth phase, changed similarly during the growth of Moraxella sp. CK-1. Extracellular cell wall lytic enzyme showed a temperature optimum of 60~70℃, a temperature stability of up to 60℃, and a pH optimum of 9.0. The lytic activity was enhanced by several enzyme stabilizers, chemicals, and metal ions except Ca^2+ and Mn^2+. Fe^3+ and Cu^2+ ions inhibited the lytic activity almost completely. The lytic enzyme was believed to be amidase or endopeptidase from the result that free amino groups in substrate solution increased when SDS-insoluble peptidoglycan was treated with extracellular enzyme(s) of Moraxella sp. CK-1.

Reverse dot hybridization 방법과 16S rRNA gene(16S rDNA)을 이용한 식품에서 식중독균의 탐색

김민성(Min-Seong Kim),신규철(Kyu-Chul Shin),이형구(Hyung-Gu Lee),한명수(Myung-soo Han),민병례(Byung-Re Min),최영길(Yong-Keel Choi) 한국식품과학회 2003 한국식품과학회지 Vol.35 No.3

Fusarium 속에서 PFGE 를 이용한 Electrophoretic Karyotyping

민병례,정진숙,최영길 ( Byung Re Min,Jin Sook Jung,Yong Keel Choi ) 한국균학회 1998 韓國菌學會誌 Vol.26 No.2

토양미생물 생태계의 발달지표에 관한 연구

안연준,한명수,민병례,최영길 ( Yeon Jun Ahn,Myung Soo Han,Byung Re Min,Yong Keel Choi ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2

To determine the successional level of soil ecosystem, microbial population was examined and environmental factors were measured. Sampling site were evergreen forests area. Among the environmental factors, total organic matter was ranged 11.880(Yesong-Ri, Wando-Gun, Jeoranam-Do)∼66.20㎎/g dried soil(Ggamak Island, Kangjin-Gun, Jeoranam-Do) in surface soil and 4.500(Mok Island, Uljoo-Kun Kyungsangbuk-Do)∼17.000㎎/g dried soi(Ggamak Island, Kangjin-Gun, Jeoranam-Do) in deep soil. Average total organic matter were 23.940 in surface soil and 11.988㎎/g dried soil in 15㎝ depth of soil. Soluble sugar content were measured from 0.045(Maengsun-ri, Wando-Gun, Jeoranam-Do) to 0.172㎎/g dried soil(Mira-ri, Wando-Gun, Jeoranam-Do) and average was 0.103㎎/g dried soil in surface. In 15㎝ depth of soil, it was ranged 0.019 (Maengwun-Ri, Wando-Gun, Jeoranam-Do)∼10.131㎎/g dried soil and average was 0.065㎎/g dried soil. On the basis of these experimental data, S/O value, the ratio of soluble sugar against total organic matter, in surface soil was ranged 0.002(Ggamak Island, Kangjin-Gun, Jeoranam-Do)∼0.095 (Jindo-Gun. Jeoranam-Do), and average was 0.005. And also it was ranged 0.001 (Maengsun-Ri, Wando-Gun. Jeoranam-Do) in lower depth of soil and average was 0.006. And all of S/O value were higher than 0.00371 in Mt. Palwang, 0.0018 in Mt. Weolchul and 0.0005 in Mt. Jukyeop. However, they were much lower than 0.0252 in Mt. Jiri and 0.16 in Mt. Kyebang. From the above result, it can be concluded that S/O value, the ratio of soluble sugar against total organic matter, in soil, was useful parameters for soil environment of climax forest in ecosystem.

Trichoderma 속의 염색체에 관한 연구

최영길,민병례,채영규 한국균학회 1985 韓國菌學會誌 Vol.13 No.4

The life cycle of 17 species of Trichoderma was elucidated to seize the proper stage for observing the nuclear behavior and chromosome count. The most convenient stage for the purpose in their life cycle was the stage just before producing the asexual spore. Of the 17 species in the genus Trichoderma the haploid chromosome numbers were counted 5, 6, 7 and 10. Six chromosomes were most frequently observed. It is believed that the basic chromosome number is placed between 4 and 10, and that the number might be 6, referring to the related papers. It appears necessary to reclassify the single genus of Trichoderma into at least two or three genera.

Restriction Fragment Length Polymorphisms of Mitochondrial DNA among Seven Fusarium Species

최영길,민병례,임경혁 한국유전학회 1997 Genes & Genomics Vol.19 No.3

We compared restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA among seven Fusarium species. Mitochondrial DNA from F. oxysporum (section Elegans), F. moniliforme and F. subglutinans (section Liseola), F. nygamai F. napiforme and F. beomiforme (section Dlaminia), F. graminearum (section Discolor ) were digested with the restriction endonucleases Eco Rl, Bglll, Hin dlll, Xbal, Pstl and Eco RV. Fragments were separated on agarose gels, used restriction analysis, Southern-blotted, and hybridized with two random probes, a 3.5 kb fragment from F. subglutinans mtDNA and a 1.6 kb fragment from F. napiforme mtDNA. RFLP analysis of mtDNA revealed high interspecific variation. The genome size of mitochondria in these seven species ranged from 50. 4 kb to 84.5 kb. Restriction fragment analysis of mtDNA indicated that F. nygamai and F. napiforme were most closely related to each other than to the other species. But mtDNA of F. beomiforme which belongs to same section was distinct from F. nygamai and F. napiforme. Southern hybridization analysis revealed no major discrepancy with restriction fragment analysis. These results suggest that F. beomiforme may not be closely related to other two species in Dlaminia.

남조분해 균류의 분리와 동정

최영길,민병례 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-

부영양화 수역의 4개 저수지로부터 곰팡이 12균주를 분리하였으며, 이들 분리 곰팡이들 중 남조류의 생장억제 능력이 우수한 HCLF-34와 HCLF-21을 왕송저수지로부터 분리하였다. 형태적인 특징에 따라 Penicillium sp.(HCLF-34)와 Acremonium sp.(HCLF-21)로 동정하였다. 두 개의 분리 곰팡이중 Penicillum sp.(HCLF-34)만이 Anabaena cylindrical lawn과 M.luteus agar p;ate에서 균체, 배양상등액 및 멸균된 배양상등액에서 plaque를 형성하였다. A.cylindrica와 Penicillium sp.(HCLF-34)의 혼합배양시 배양 3일 후 남조류의 분절 및 sheath의 손상이 관찰되었고, A.cylindrica와 Penicillium sp.(HCLF-34) 혼합배양시 배양 10분 후부터 남조류의 분절 및 sheath의 손상이 관찰되었다. The fungi that hydrolyze the blue green algae were isolated from Wangsong reservoir shown in eutrophication and identified as the genera Acremoniwm sp., and Penicillium sp. By morphological characters. The Penicillium. sp.generally showed less lytic activity than the Acremonium sp. on the Anabaena cylindrical agar lawn, but the cultured supermatant of Penicillium sp. Exhibited greater activity than those of the Acremonium sp. On Micrococcus luteus. In the mixed culture of bule green algae(Anabaena cylindrical) and the fungi(HCLF-34), the blue green algae was segmented and lysed in 3 days. In addition, the blue green algae(Anabaena cylindrical) was segmented and the width sheath of Anabaena cylindrical was appeared as lysed on the scanning electron microscopy, in mixed culture between the algae and fungi.

자외선 살균효과에 미치는 옥수(玉水)와 옥분말(玉粉末)의 영향

최영길,민병례 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-

생체에 대한 옥수와 옥분말의 자외선 차단효과를 알아보기 위하여 동물의 피부 생태계와 연관있는 미생물들(Gram+, Gram-, 및 Eucaryotic yeast)을 대상으로 실험한 결과 다음과 같은 결과를 얻었다. 1.자외선 조사후 생존한 미생물 개체수의 측정결과로 분석해 볼 때 옥수첨가 액체배지에서 보다 옥분말 첨가 액체배지에서 자외선 차단효과가 높게 나타났다. 2.자외선 차단효과는 Escherichia coli, Saccharomyces cerevisiae, Bacillus subtilis의 배양체의 순으로 확인되었다. This experiment was designed to elucidate the effect of jade product (jade powder and jade water) on the sterilization by ultraviolet ray as the ecological model of animal skin. The microorganisms(Gram+, Gram-, and Eucaryote) which are closely related to animal skin were cultured in broth medium with jade powder and jade water, illuminated by ultraviolet ray to count viable number of microorganism, and evaluated the effect of jade product on the strength of ultraviolet ray. The results of this experiment are summarized as the followings. 1.The survival number of microbes illuminated by UV light were higher in the cultures of jade powder than those in the jade water, It could be evaluated that jade powder has an effect to interrupt the sterilization of UV light. 2.The sterilization by ultraviolet ray was decreased in order of the cultures as the Escherichia coli, Saccharomyces cerevisiae, and Bacillus subtilis.