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Multiplex Real-Time PCR을 이용하여 6종의 주요 잇몸질환 유발 미생물을 동시에 검출하는 기법
조홍범,Cho, Hong-Bum 한국미생물학회 2013 미생물학회지 Vol.49 No.3
This study utilized an analysis method for detecting six microorganisms, such as Actinobacillus actinomycetemcomitans, Campylobacter rectus, Porphyromonas gingivalis, Tannerella forsythus, Treponema denticola, and Prevotella intermedia, triggering periodontal disease, using multiplex real-time polymerase chain reaction (PCR). The analysis including internal control was made by dividing the six species into two groups using four fluorescence dyes, and it was verified that there was no interference or cross-reaction between the target species and different kinds of oral microbial species. Qualitative and quantitative analyses were conducted on each microorganism in various samples, such as saliva and the plaque, using the multiplex real-time PCR and comparative analysis between periodontitis patients and healthy people, revealing obvious differences between them. 본 연구는 multiplex real-time PCR을 이용하여 Actinobacillus actinomycetemcomitans, Campylobacter rectus, Porphyromonas gingivalis, Tannerella forsythus, Treponema denticola, Prevotella intermedia 등과 같은 6종의 주요 치주 질환 원인 미생물들을 동시에 검출할 수 있는 분석 방법에 관한 것이다. 4개의 형광 염료를 사용하여 internal control과 함께 3개의 균종씩 나누어 분석하였으며, 분석 대상 균종 간 그리고 다른 종류의 구강 미생물 균종과의 간섭과 교차 반응이 없음을 확인하였다. 본 연구의 multiplex real-time PCR은 타액과 플라그 등의 다양한 샘플에 포함되어 있는 각 미생물들을 정성, 정량적으로 분석할 수 있었으며, 치주염 환자와 건강한 사람들에 대한 비교 분석 결과 분명한 차이를 발견 할 수 있었다.
SPSS를 활용한 플라이애시 혼입 콘크리트의 효율적 배합 제안
조홍범(Cho Hong-Bum),지남용(Jee Nam-Yong) 대한건축학회 2011 大韓建築學會論文集 : 構造系 Vol.27 No.1
For efficient mix design, this paper offers following information: the fly ash (FA) replacement ratio in which strength development is the maximum, the range of FA replacement ratio in which the strength of FA concrete is higher than that of ordinary Portland cement (OPC) concrete, and the binder content per unit strength. For these matters, various statistical analyses were performed based on a wide range of mix proportions and a number of strength test results of ready mixed concretes used in eight construction sites. The strength ratio, the ratio of FA concrete to OPC concrete in compressive strength, was expressed as the function of binder-water ratio and FA replacement ratio by multiple linear regression analysis. This equation suggests the range of FA replacement ratios in which strength ratio of 1 or higher can be obtained when binder content of FA concrete is the same as that of OPC concrete. The strength ratio increases with increasing water-binder ratio. As water-binder ratios decrease, the range of FA replacement ratios also decreases. It is desirable that FA concrete should have FA replacement ratio of about 15% to guarantee required average compressive strength using minimum binder contents. In FA replacement ratio of about 15%, the binder content per unit strength is 10.1∼10.6㎏/㎥/㎫ with respect to water-binder ratio. Because the binder content per unit strength is calculated with respect to water-binder ratio and FA replacement, its concept can be used as a means of determination of the binder content in mix design of FA concrete.
실리카흄의 겉보기 밀도에 따른 80MPa급 고강도 콘크리트의 경화 전후 물성
조홍범 ( Cho Hong-bum ),김영선 ( Kim Young-sun ),전현수 ( Jeon Hyun-soo ),손원일 ( Son Weon-il ),석원균 ( Seok Won-kyun ),이재명 ( Lee Jae-myung ) 한국건축시공학회 2023 한국건축시공학회 학술발표대회 논문집 Vol.23 No.2
In the production of high-strength concrete of 80 MPa or more, silica fume is widely used as a binder to ensure the strength safety and pumpability of concrete. The bulk density of silica fume is an important physical property that can have a significant impact on the performance of concrete. Therefore, Understanding the effect of silica fume density on the physical properties of concrete and selecting the appropriate material with the correct density is crucial to ensuring optimal performance in construction projects.
조홍범 ( Hong Bum Cho ),정순오 ( Soon Oh Jeung ),최용근 ( Yong Gun Choi ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.1
동굴 내 정점별 세균 군집 구조를 분석하기 위하여 PCR amplified 16S rDNA denaturing gradient gel electrophoresis (DGGE)를 적용하였다. DGGE는 동일한 분자량을 갖는 dsDNA band라고 할지라도, 각각의 염기서열 차이에 따라 전기영동 상에서 고유한 band 양상을 나타낼 수 있다. eubacteria의 16S rDNA V3 region을 증폭하기 위해 GC341F와 PRUN518r을 primer로 사용하여 지하수내에 미생물 군집의 다양성과 유사성을 분석하였다. DGGE band양상을 통해 동굴내의 세균 군집 구조는 외부 환경에 비해 상대적으로 종다양성이 낮으며 동굴내에서 특이적으로 서식하는 종이 있음을 확인하였다. 또한 유기 영양물질의 공급이 제한되어 있는 동굴에서 구아노가 주요 유기 영양물질의 공급원으로서 큰 영향을 미치고 있는 것으로 파악되었다. DGGE상의 일부 band의 염기서열분석 결과 Pseudomonas sp. NZ060과 Pseudomonas pseudoalcaligenes, uncultured Variouorax sp,. soil bacterium NS7로 동정되었다. The bacterial community of water stream, soil and guano in Gossi cave was examined by using PCR amplified the 16S rDNA-denaturing gradient gel electrophoresis (DGGE). In this study, the genetic diversity and the similarity of bacterial community between open area and non-open area for cave tour were investigated, and the seasonable variation pattern was compared each other. DGGE is attractive technique, as it separate same length dsDNA according to sequence variation typical 16S rDNA genes. The diversity and similarity of bacterial community in cave was analyzed by GC341f and PRUN518r primer sets for amplification of V3 region of eubacteria 16S rDNA. The specific DGGE band profile of the cave water gives the possibility that the specific bacterial cell can be adapting to the specific cave environment and living in the cave. The DGGE band profiles of all samples with guano were compared and analyzed by image analyzer, in which mutual band profile was compared to be and the band intensity of guano was the highest. From these result, it is thought that the guano was main nutrient source and influenced on the community structure of the cave environment where is nutritionally limited. Pseudomonas sp. NZ060, Pseudomonas pseudoalcaligenes, uncultured Variovorax sp. and soli bacterium NS7 were identified to be on some sample from analysing DNA sequence of some DGGE band.