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경북서부지역 설사자돈에서 분리한 Haemolytic E. coli의 생화학적 특성 및 약제 내성
조종숙 ( Jong Suk Cho ),이정아 ( Jeong Ah Lee ),오강희 ( Gang Hee Oh ),박영구 ( Young Goo Park ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2
The present study was conducted to investigate the biochemical characteristics and antibiotic resistance of pathogenic Escherichia coli (E. coli) isolated from piglets with diarrhea in Kyongbuk Western Area during the period from February to November 1992. 55 E. coli strains were isolated from 97 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. The majority of E. coli were susceptible to amikacin, enrofloxacin and gentamicin. 51(92.7%) out of 55 drug resistance stains carried R factor (R+) which were transferable to the recipients by conjugation.
경북지역에서 수거된 식육의 Listeria sp 오염실태 조사
조종숙 ( Jong Suk Cho ),김유정 ( Yoo Jung Kim ),김성숙 ( Seong Suk Kim ),도재철 ( Jae Cheul Do ),김석한 ( Suk Hwan Kim ),이창우 ( Chang Woo Lee ),김인성 ( In Sung Kim ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.4
Nowadays we continue to face challenges to the safety of our foods. It seems clear that contaminated meats provide the major route of listeria monocytogenes transmission from the environment to humans. L monocytogenes is the most important species associated with disease in humans among the listeria sp. The current incidence of symptomatic listeriosis caused by L morzocytogenes is uncertain. Although the number of reports in the literature on listeriosis are increasing, it is likely that they are actually unrecognized or underreported because of a lack of awareness on the part of some laboratory workers who fail to isolate or identify these organisms. To get the information of sanitary development, we survey various meats (beef, pork, etc) in Kyongbuk area. Samples were collected from local meat shop at Kyongbuk area. Total sixty six case were isolated and identified from one hundreds and seven samples. L innocua was the most abundant in listeria sp. Among 66 isolates, the number of isolated L innocua was 43(65.2%). The numbers of isolated L murrayi, L welshimeri, L monocytogenes and L seeligeri were 12 (18.2%), 7 (10.6%), 3 (4.5%) and 1 (1.5%), respectively, but L grayi, L ivanovii were not isolated.
국내 한우의 소바이러스성 설사 바이러스 지속감염우에 대한 실태 조사
조종숙 ( Jong Suk Cho ),김경동 ( Gyung Dong Kim ),박홍제 ( Hong Je Park ),임연수 ( Yeoun Su Lim ),홍성희 ( Sung Hee Hong ),서창원 ( Chang Won Seo ),류희정 ( Hee Jeong Ryu ),신령자 ( Ryeong Ja Sin ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.2
Bovine viral diarrhea virus (BVDV) is very important disease in domestic and wild ruminants and has a world wide distribution. Cattle persistently infected with BVDV (BVDV-PI) are the primary reservoir for BVDV infection in Korean native cattle herds. The prevalence of cattle persistently infected with BVDV (BVD-PI) was determined using 4,260 heads from 29 Korean native cattle farms at 8 districts from 2011 to 2012. The sera and ear nothches were collected for each sample. We surveyed BVD-PI cattle using antibody ELISA and antigen capture ELISA for detection of antibody and antigen respectively. Three thousand seventy-six cattle (72.2%) were positive for BVDV antibody and a total of 27 BVD-PI cattle were found in 12 farms. 11 cattle (40.7%) out of the total 27 BVDV-PI cattle were six months old or under. The positive rate of BVDV antibody (83.2%) from 12 farms with BVD-PI cattle was higher than the positive rate of BVDV antibody (63.6%) from 17 farms without BVD-PI cattle.
육계에서 조직과 혈액의 enrofloxacin 및 대사성 ciprofloxacin 잔류 상관관계 조사 및 검사방법간의 비교
성명숙 ( Myung Suk Sung ),김미숙 ( Mi Suk Kim ),서희진 ( Hee Jin Seo ),배동록 ( Dong Rok Bae ),황지영 ( Ji Young Hwang ),김순태 ( Soon Tae Kim ),조종숙 ( Jong Suk Cho ),박홍제 ( Hong Je Park ),홍성희 ( Sung Hee Hong ),김경동 ( Gyu 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4
The purpose of this study was to evaluate detecting methods and the relationship between tissues and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens. Two groups of broiler chickens were administrated via the drinking water with 50 μg/mL and 100 μg/mL of enrofloxacin for 5 days, respectively. The concentration of enrofloxacin and metabolic ciprofloxacin in tissues (muscle and kidney) and blood were measured during administration period (for 5 days) and withdrawal period (for 12 days) by high performance liquid chromatography (HPLC) method. Also, all samples were conducted for screening of residues by microbial method using E. coli for quinolone detection and immuno- chromatography method using Smart kit. The relationship between tissues (muscle and kidney) and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens was followed : The levels of enrofloxacin and metabolic ciprofloxacin residues in muscle and kidney were higher 2.9∼3.2 folds, 3.6∼3.8 folds more than the residues levels in blood, respectively. These results support we can predict the residues in muscle and kidney from the residues in blood. In comparison of detecting methods for antibiotic residues, microbial method using E. coli for quinolone detection and immuno-chromatography method using Smart kit could detect positive reaction at similar or lower concentration than violative concentration of enrofloxacin and metabolic ciprofloxacin in chicken tissues. These results support what two screening methods are useful for screening of quinolone detection in chickens.
Rat에 sulfamethazine sodium 경구투여 후 시간경과에 따른 혈청, 간장 및 근육내 잔류함유량 변화
도재철 ( Jae Cheul Do ),장지택 ( Jee Taek Jang ),이영미 ( Young Mi Lee ),조종숙 ( Jong Suk Cho ),조민희 ( Min Hee Cho ),박희주 ( Hee Ju Park ),신대길 ( Dae Gil Shin ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.2
Sulfamethazine sodium was orally administrated to sprague-dawley strain male rats(body weight, 200-300g) with using sonde at the rate of 20 mg/100g body weight (recommended therapeutic dose) on once a day for 3 days. There were investigated the depletion rate of the suifaniethazine in serum, liver and skeletal muscle of rat at the time 8 hours, 1st, 2nd, 3rd, 4th, 5th and 6th day after administration sulfamethazine sodium. 1. The mean concentrations of sulfamethazine in serum according to the time lapsed after oral administration of the sulfamethazine sodium were showed 215.53 ± 42.99 ppm at the 8 hours after withdrawal of medicated sulfamethazine. And gradually according to the time lapsed, the concentrations of sulfamethazine residues in serum were significantly (p<.05) decreased 25.87 ± 5.18 ppm at 1st day, 2.30 ± 0.61 ppm at 3rd day and 0.11 ± 0.02 ppm at 6th day after withdrawal of medicated sulfamethazine. 2. The mean concentrations of sulfamethazine in liver were significantly (p<.05) decreased 81.77 ± 12.88 ppm to 0.11 ± 0.03 ppm between 8 hours and 6th day according to the time lapsed after oral administration of the sulfamethazine sodium for 3 days. 3. After oral administration of the sulfamethazine sodium, the mean concentrations of sulfamethazine in skeletal muscle were significantly (p<.05) decreased 35.96 ± 1.39 ppm to 0.009 ± 0.001 ppm between 8 hours and 6th day after withdrawal of medicated sulfamethazine.At the 4th day, the concentrations of sulfamethazine residues were showed 0.10 ± 0.04 ppm below 0.1 ppm at the permitted limit concentration of muscle in Korea 4. After oral administration of the sulfamethazine sodium once a day for 3 days, there were showed the highest concentration in serum (215.53 ± 42.99 ppm) than in liver (81.77 ± 12.8 ppm) and skeletal muscle (35.96 ± 1.39 ppm) at the 8 hours after withdrawal of medicated sulfamethazine. The mean concentration of sulfamethazine residues in serum, liver and skeletal muscle were gradually decreased according to the time lapsed.