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엄성심 ( Sung Shim Eum ),이정원 ( Jeoung Won Lee ),서이원 ( Won Seo Lee ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3
Classical swine fever(CSF) was confirmed in 19 herds in Jeunbuk provence(Iksan, Gimje, Wanju, Buan, and Jangsu) in Korea between March and May, 2003 and 10,263 pigs were slaughtered. Pigs contacted with CSF virus in primary outbreak farm show fever, reduced appetite, arched back and chill in company with sever respirative sign and then most infected farms also were observed to fever, reduced appetite, sudden death, and leukopenia(101 pigs). In order to detecting infectious pig with CSF virus, A total of 555 pigs were inspected in 65 herds and blood samples were collected and serological test(ELISA), antigen ELISA, and reverse transcription-polymerase chain reaction(RT-PCR) had been done. Positive rate were 74%(410 pigs) in antibody ELISA, 2%(11 pigs) in antigen ELISA and 33%(182 pigs) in RT-PCR, respectively. As shown that the RT-PCR was useful than the ELISA for determining CSF virus in blood, meat, and other organs.
이정원 ( Jeoung Won Lee ),엄성심 ( Sung Shim Eum ),박인규 ( In Gyu Park ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.2
Newcastle disease(ND), infectious bronchitis(IB), low pathogenic avian influenza(LPAI) and fowl typhoid(FT) have been known as egg drop laying diseases because of the serious layer damage from mass zone layer. In this study, such egg drop laying diseases were investigated. To access this study, we performed to evaluate antibody titers in serum and isolated bacteria and virus from organs and feces on May, July and September in 2003. The distribution of ND from January to May, IB and LPAI from October to February of the next year, and FT from March to September were inspected by the question survey in 21 farms. ND revealed to be positive rates of 490 to 474(96.7%) in May, 510 to 506(99.2%) in July and 510 to 510(100%) in September with hemagglutination inhibition(HI) test. The mean antibody titers were 10.2, 9.9 and 10.2, respectively. With regard to IB, 484 out of 490 samples(98.7%) in May, 508 of 510(99.6%) in July and 509 of 510(99.8%) in September showed positive results and the mean antibody titers were gradually increased with 8.2, 9.0 and 9.4, respectively. According to HI test of LPAI, the positive results were shown in 442 of 480(92.1%), 394 of 494(79.8%) and 402 of 483(83.2%) in May, July and September, respectively. The mean antibody titers were decreased with 4.6, 4.3 and 4.0. The distribution of LPAI also elicited the positive rates of 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September as determined by ELISA and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. Compared ELISA results with HI test of LPAI the positive results were 480 to 422(92.1%), 475(99.0%), 494 to 394 (79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, the positive rate determined by ELISA was higher than that of HI test with 6.9, 18.4 and 14.5%, respectively. When performed RT-PCR for ND using organ and feces samples, the pathotypes were detected 5(15.6%) in May, 2(5.3%) in July, 2(7.1%) in September but there is no samples showing positive band for LPAI. In attempt to isolate Salmonella gallinarum, bacteria were obtained from 4 cases(12.5%) in May, 9(23.6%) in July, 5(17.8%) in September. Thus the highest rate for isolation revealed to be shown in July. When evaluated the antimicrobial susceptibility to 18 isolated strains of S. gallinarum, bacteria were sensitive to trimethoprim/sulfamethox(61.1%), kanamycin(55.5%), ampicillin(55.5%) and amoxacillin/clavulanic acid(55.5%), cephalothin(50.0%), but resistant to penicillin(88.9%), streptomycin(88.9%), erythromycin(83.4%) and tetracycline(61.1%). According to HI test of ND and LPAI using captured 164 wild Korean tree sparrows(Passer nontanus), the positive rates were 47.6% and 57.3%, and the mean HI titers were 5.32 and 4.02, respectively. 71(43.2%) and 58(35.3%) in captured sparrows also showed more than 4 titers for HI test to ND and LPAI, respectively. However, the attempt for isolation of viruses failed in all samples.
유산균 발효유 및 starter에서 항생제내성 유산균 분리 및 전달성 R plasmids 분포조사
백귀정 ( Kui Jeong Baek ),서형석 ( Heyng Seok Seo ),노영선 ( Young Sun Roh ),양해동 ( Hae Dong Yang ),허부홍 ( Boo Hong Hur ),서이원 ( Lee Won Seo ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
Plasmids are covalently closed circular molecules of DNA that are stably inherited and replicate somewhat independently of the bacterial chromosome. Genes carried on plasmids can mediate a wide variety of important functions, including antibiotics(R plasmids) and heavy metals resistance, toxins production, cell penetration, iron chelation, complement resistance, and metabolic characteristics such as sucrose and lactose fermentation. Fifty strains of lactobacilli were isolated from 26 staters and 29 fermented milk products. They were classified 27 strains as Lactobacillus paracasei subsp paracasei , 11 stains as Lactococcus lactis subsp cremoris, 6 strains as L delbrueckii subsp lactis, 4 strains as L acidophius, and 2 strains as L delbrueckii subsp bulgaricus. All of these strains were examined for drug resistance and transferability of R plasmids. All of the isolates were sensitive to Am, C, CF, E, NB, P, T, and Te. But resistant to SXT 94%(47 strains), K 66%(33 strains), S 56%(28 strains), ENR 50%(25 strains), NOR 38% (19 strains) CIP 38%(19 strains), GM 16%(8 strains), and N 14%(7 strains), in order. And 32 different resistant patterns were found. The most frequently encountered patterns were CIP-ENR-K-NOR-S-SXT (5 strains). In vitro R plasmids transfer experiment, 57 antibiotic resistant strains which were not trans-fer to the recipient 2 Escherichia coli strains by conjugation. These results indicate that Lactobacillus in internal trade market` stater recognize R factor but transmissible R plasmid is not existed.
전주지역 애완견에서 심장사상충, 개선충 및 모낭충 감염실태 조사
이정원 ( Jeoung Won Lee ),엄성심 ( Sung Shim Eum ),박인규 ( In Gyu Park ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.1
A survey on the prevalence of heartworms, scabies and demodicosis of 1,011 pet dogs in 5 animal hospitals Jeonju area was performed from June to October 2004. The result obtained from this experiment were summarized as follows; 1. The 1,011 pet dogs coming to help the animal hospital more than disease of pleonephritis 62(6.1%), enteritis 75(7.4%), dermatitis 62(6.1%), scabies 55(5.4%), heartworms 43(4.3%), tympanitis 41(4.1%), demodicosis 22(2.2%), gastritis 21(2.2%), fracture 9(0.9%), respectively. 2. The 307 examined pet dogs were heartworms antigen positive to 43(14.0%), Microfilaria 31(10.1%) and the kinds of mixed breeds 34 (antigen and Microfilaria 12), Jindo 19(antigen 7, Microfilaria 6), respectively. 3. Heartworms antigen positive of examined 34 pet dogs mean ages 4.3 and general blood cell count mean of WBC 20.5×103/㎣, RBC 61.3×106/㎣, HGB 6.27g/㎗, HCT 20.9%, PLT 13.0×103/㎣, MCV 33.2㎛3, MCH 39.4pg, MCHC 14.8g/㎗, RDW 280. 1%, MPV 9.2㎛3, respectively. but showed a few high normal value WBC, MCV, MCH and a low MCHC. 4. This experiment was 1,011 pet dogs to dermatitis 139(13.7%) of scabies 55(39.6%), demodicosis 22(15.8%), respectively.
권미순 ( Mee Soon Kwon ),이지영 ( Ji Yoog Lee ),박인규 ( In Gyu Park ),윤여백 ( Yeo Baik Yoon ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
The present study was done to investigate the effect of dietary supplemental freezer dry powder fermentation soybean by Bacillus subtilis(natto) on the growth performance and intestinal microflora, prevention of fowl typhoid infection, the uptake of vegetative diet in broiler chickens. The chickens were fed control diet(supplement antibiotics) and fermentation soybean(0.75, 1.5, 3%) diets. A total of 280 one day old broiler chickens with randomly mixed sexes were fed the four diets for 6 weeks. Body weight gain of chicken fed 1.5% fermentation soybean by B subtilis tend to increase higher than the control from 6th week. Chickens fed diets containing 1.5% fermentation soybean by B subtilis had higher intake than those fed the other levels to the 3th weeks, but lower than control from the 4th week to the 6th week. Feed conversion also improved significantly in the supplemental 1.5% fermentation soybean by B subtilis from the 4th week to the 6th week. The number of B subtilis and Lactobacillus spp in the ileum and cecum tend to increase in the supplemental fermen-tation soybean by B subtilis at 6 week of age, but was not significantly different. In the nutrient digestibility, the feed conversion on the supplemental 1.5% fermentation soybean by B subtilis was better than the control and the weight of drying feces lower than the control. In test of S gallinarum intramuscular inoculation, reisolation rate of S gallinarum in liver and feces 1.5% the fermentation soybean by B subtilis 75%(liver), 17%(feces) had decreased than the control.
LC/MS를 이용한 식육중 잔류 벤지미다졸계 구충제 분석법 연구
최은영 ( Eun Young Choi ),서형석 ( Heyng Seok Seo ),백귀정 ( Kui Jeong Baek ),허부홍 ( Boo Hong Hur ),서이원 ( Lee Won Seo ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.1
Recently, mass spectrometry coupled with liquid chromatography(LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra C18 column with 0.01% trifluoroacetic acid-acetonitrile(TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with 50% acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via [M+H]+ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles
산란계 밀집지역에서 혈구응집억제반응과 효소면역측정법을 이용한 가금인플루엔자 혈중항체가의 비교 조사
이정원 ( Jeoung Won Lee ),엄성심 ( Sung Shim Eun ),이성재 ( Sung Jae Lee ),서이원 ( Lee Won Seo ),서석열 ( Surk Yul Seo ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1
This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA), the HI titer and mean ratio S/P ratio) of avian influenza virus. To perform this study, the 1,457 sera of layers 21 farms in May, July and September, respectively. As a result of HI test, positive rates were 480 to 422 (92.1%) in May, 494 to 394(79.8%) in July and 483 to 402(83.2%) in September, and the mean antibody titer were 4.6, 4.3, 4.0 to 0.3 decreased, respectively. The positive rates by ELISA, 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September, and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. The result of HI test and ELISA positive 480 to 422(92.1%), 475(99.0%), 494 to 394(79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, ELISA was shown more sensitive compare the HI titers.
HPLC/MS을 이용한 aminoglycoside 항생제동시 분석에 관한 연구
노영선 ( Young Sun Roh ),백귀정 ( Gui Jung Baek ),김승용 ( Seung Yong Kim ),최은영 ( Eun Young Choi ),서형석 ( Heyng Seok Seo ),허부홍 ( Boo Hong Hur ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics(AMGs) using Heptafluorobutyric acid(HFBA) as a ion-pairing reagent. AMGs(amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry(ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.
정영미 ( Young Mee Jung ),김기주 ( Ki Joo Kim ),엄성심 ( Sung Shim Eum ),이지영 ( Ji Young Lee ),노영선 ( Young Sun Rho ),서석열 ( Surk Yul Seo ),박정배 ( Jong Bae Park ),이희문 ( Hee Moon Lee ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1
This survey was performed to investigate avian salmonellosis from broiler farms in Namwon area for 4 years. The whole samples from farms were 541 and chicken samples were 294. Out of 294, 83 cases were identified with avian salmonellosis for 4 years by various serological and biochemical tests. There was no outbreak of pullorum disease, but fowl typhoid occurred in 36 farms. The outbreak of avian salmonellosis occurred continuously regardless of seasons throughout the year. Avian salmonellosis mostly happened within 10 days of age which were 52 cases(63%). The selection of susceptible antibiotics has changed from Quinoline group in 2000 to amoxacillin+clavulanic acid(AmC).