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      • 다단 육묘시설에서 경량상토의 묘삼생산성 및 묘소질

        장명환,이응호,김영준,김승한,최양애,원도연,정지윤,김임수 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.05

        Background : Multi-stage system were used for development of stable ginseng seedling production. Bed-soil for the production of ginseng seedling in the multi-stage was similar to the conventional bed-soil but the weight of the bed-soil was high and the efficiency of operation in the multi-stage was lowered. In this study, the yield and quality of ginseng seedling was investigated by commercial lightweight bed-soil in the multi-stage facilities, and the possibility of application of lightweight bed-soil. Methods and Results : This study was carried out by a 3-stage cultivation bed using a 50 ㎜ sandwich panel in a house shaded with 85% light-shielding net. The width of the cultivating bed was 90 ㎝, the height was 30 ㎝, and the height of each stage was 50 ㎝. In the first and second stages, the amount of light was insufficient, so two rows of fluorescent lamps were installed and the third stage was used natural light. Ginseng seeds were sown on the cultivating bed in November 2016, and ginseng seeds (native species) were sown with a density of 3 × 3 ㎝. The chemical properties of lightweight bed-soil were pH 5.11, and EC 0.76 dS/m. It was suitable for ginseng seedling cultivation. The bulk density was 0.21 Mg/㎥. Among the growth characteristics of the ginseng seedlings, the root length was the longest as 17.0 ㎝ in the conventional cultivation, and the second stage was the longest at 14.8 ㎝ in the multi-stage facility. The root diameter in the multi-stage system was 0.2 - 0.4 ㎜ thicker than the conventional one. Root weights of lightweight bed-soil were similar to those of conventional cultivation. The yield of ginseng seedlings in the 1 st, 2 nd and 3 rd stage was 721.3 g, 692.0 g, and 394.7 g/1.62 ㎡ respectively. Conclusion : In the production of ginseng seedling using multi-stage facilities, the commercial bed-soil was better than the conventional bed-soil (light, workability). The differences in yields in the multi-stage facilities can be overcome if the growth management such as moisture management is more systematic. If we develop the technology to reuse the bed-soil after harvesting the ginseng seedling, it will be economical and able to supply to farmers.

      • KCI등재

        Theobromine, a Methylxanthine in Cocoa Bean, Stimulates Thermogenesis by Inducing White Fat Browning and Activating Brown Adipocytes

        장명환,강남현,술라그나 무케르지,윤종원 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.6

        Natural medicinal compounds to treat obesity have recently attracted a great deal of attention because of the serious side effects of synthetic anti-obesity drugs. Recent advances have been made to identify natural products showing thermogenic activity, which is responsible for energy expenditure in brown or brown-like (beige) adipocytes. Here, we explored the thermogenic effects of theobromine, one of the most abundant methylxanthines in cocoa, on 3T3-L1 white adipocytes and HIB1B brown adipocytes. Theobromine markedly increased the expression levels of brown-fat signature proteins (PGC-1α, PRDM16, and UCP1) and beige-specific genes (Cd137, Cidea, Cited1, Tbx1, and Tmen26) in 3T3-L1 white adipocytes and remarkably elevated the expression levels of brown fatspecific genes (Cidea, Lhx8, Ppargc1, Prdm16, Ucp1, and Zic1) in HIB1B brown adipocytes. Theobromine also reduced the expression of the key adipogenic transcription factors, C/EBPα and PPARγ, in white adipocytes, while enhancing their expression in HIB1B cells. In addition, theobromine regulated lipolytic events and fat oxidation by upregulating the expression of pACC, ATGL, pHSL, ACOX, and CPT1. Additional mechanistic study revealed that theobromine activates β3-AR and AMPK. In summary, our results provide evidence for the first time indicating that theobromine has a potential beneficial effect on browning of white adipocytes and improves lipid catabolic metabolism in both cultured white and brown adipocytes via β-adrenergic signaling and AMPK activation. Consumption of theobromine may be a feasible way to activate thermogenesis and improve systematic lipid metabolism to protect against obesity and other metabolic disorders.

      • 프랙탈의 반복함수계를 이용한 웨이블릿 기반 워터마킹 기법 연구

        장명환,황선태,이 형 대전대학교 산업기술연구소 2000 산업기술연구소 論文集 Vol.11 No.2

        본 논문은 프랙탈의 반복함수계를 이용한 웨이블릿 기반의 워터마킹 기법을 제안한다. 제안한 방법에서는, 3-단계 웨이블릿 변환을 통하여 이미지를 주파수 대역으로 변환한다. 워터마크는 반복함수의 출력벡터의 수를 조절하여 생성한다. 견고성 향상을 위해 고주파수 대역뿐만 아니라, 저주파수 대역인 LL 대역에도 워터마크를 삽입한다. 또한, 워터마킹 영상의 화질저하를 줄이기 위해 웨이블릿 변환 단계마다 가중치를 다르게 부여한다. 실험결과, 기존의 Cox 방법과 비교할 때 잡음첨가, 부분 삭제 그리고 필터링등의 공격들에 대하여 화질과 견고성면에서 향상된 결과를 보였다. In this paper, the watermarking technique using the Iterated Function System based on wavelet transform is proposed. In this method, the image is chanced into coefficients of frequency domain by 3-level wavelet transform. We adjusted the number of Iterated Function Output Vectors and then generated watermarks. We intended to improve the robustness of the watermark by embedding the watermark not only into high-pass band(LH, HL, HH) but into low-pass band(LL). Also the scaling parameters are used differently in each wavelet transformation levels in order to reduce the distortion of watermarked image. The experimental results show that our proposed method has better image quality and robustness to the attacks of noise addition, cropping and filtering than those of existing Cox's method.

      • 엽면시비제의 연용이 토양화학성과 인삼생육에 미치는 영향

        장명환,김승한,최양애,원도연,정지윤,김임수 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10

        Background : Foliar fertilizer materials are used in the field of ginseng farming to enhance the growth of ginseng. The law for ginseng industry prohibits the use of chemical fertilizer as a material for growing ginseng, but in recent years, various organic materials derived from natural materials have been produced and used in farming sites. The purpose of this study was to investigate the effects of foliar fertilizer on the growth and soil properties of ginseng during continuous use. Methods and Results : For the test materials, native seedlings were used and the amino acid solution, liquid silicate, microbial liquid and fermentation enzyme were treated from 2 to 5– year-old ginseng after transplantation. The test plots were carried out to 3 repetitions by randomized block design. The area of one plot was 3.24 ㎡. The treatments were foliar application three times at intervals of 10 days from the late of May when leaf development was completed. The chemical properties of each foliar fertilizer pH was the highest at 9.63 for liquid silicate and lowest at 5.85 for amino acids. Ammonium nitrate had the highest amino acid content (56 ㎎/ℓ). Phosphorus was the highest with 113.7 ㎎/ℓ of fermentation enzyme. As a result of foliar fertilization for 4-years up to 5-years of ginseng, the pH was similar in soil chemical properties. EC and nitrate nitrogen contents tended to be higher in foliar application than in non-treatment. In the photosynthesis of ginseng, fermentation enzyme and microbial treatment tended to be high. Thickness of ginseng leaf was the thickest in microbial treatment. Microbial treatment was the highest in the underground part. Soil chemical properties were also affected but there was no significant change to inhibit the growth of ginseng. Conclusion : The treatments of the foliar fertilizer were more effective than the no treatment on the growth of ginseng. Among the foliar fertilizers distributed on the market, those derived from natural products can be used in ginseng cultivation.

      • KCI등재

        In Vitro Evaluation System for Varietal Resistance against Ripe Rot Caused by Colletotrichum acutatum in Grapevines

        장명환,문용선,노정호,김승희,홍성기,윤해근 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.1

        Ripe rot caused by Colletotrichum spp. occurs severely in grapevines grown in warm and humid climatic regions such as Korea. Research was initiated to develop an efficient screening system for grapevine resistance against ripe rot through the inoculation of C. acutatum spore suspensions grown in potato dextrose agar. Resistant grapevine cultivars against ripe rot were easily distinguished from susceptible ones through lesion scorings of the detached leaves and immature fruits inoculated with mycelial blocks or spore suspension of C. acutatum at 25℃. Resistant grape cultivars identified through pathogen inoculation include: ‘Zabalkanski’, ‘Benianyo’, and ‘Beniyamabico’. The evaluation of grapevines varietal resistance against ripe rot revealed some specificity in the culture filtrates for the pathogens. Hence, the application of culture filtrates treatment of C. acutatum can be an alternative approach for screening the ripe rot varietal resistance in grapevines.

      • KCI등재

        롱월채탄 적용과 메탄가스 및 지반침하 예측기술 소개

        장명환 한국자원공학회 2012 한국자원공학회지 Vol.49 No.4

        Long-wall coal mining method is using a large scale automatic equipment for coal production and it makes tens of thousands per day at the intensive mine site. Before we carrying out long-wall mining method, many research and pre-processing are necessary to be performed. Among those processes, ventilation and exhaust system is the most important part to emit methane gas of coal bed. Methane gas emission is necessary to perform efficiently in the short or long-term during the development or pre-development period. About long-wall mining method, there are many studies of methane gas control system were conducted. The studies were using input data with variety of conditions for using artificial neural network systems which can help us to infer methane gas flow rate and concentration. And also, when we using Long-wall mining method, the subsidence is inevitable. However, if we could estimate the volume and scale of subsidence at right time, we could minimize the damage which caused by subsidence. 롱월채탄은 대규모 자동화 기계장비를 사용하여 집약된 작업장에서 하루 수 만톤의 석탄을 생산하는 시스템이다. 롱월채탄을 수행하기 이전에 많은 조사와 전처리 과정을 수행하게 되는데 이중에서 중요한 요소가 탄층의 메탄가스 등을 배출시키기 위한 통기 및 배기시스템이다. 메탄가스배출은 개발이전 또는 개발 중 장단기 기간중에 효율적으로 수행할 필요가 있다. 롱월에서 메탄가스 제어시스템은 많은 연구가 이루어 졌으며, 주변의 여러 가지 조건들을 입력자료로 하여 최종적으로 메탄가스 유동량과 농도를 추론하는 인공신경망시스템 등을 활용하는 방법등이 있다. 또 롱월채탄은 지반침하가 필연적으로 이루어지는데, 시기적절한 침하량과 침하규모 등을 예측함으로써 지반침하에 의한 피해를 최소화 시킬 수 있다.

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