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재래산양의 계절적 무발정기의 혈중 Progesterone 의 변화
이지삼,곽대오,박충생 ( J . S . Lee,D . O . Kwack,C . S . Park ) 한국축산학회 1985 한국축산학회지 Vol.27 No.12
This experiment was conducted to investigate the breeding seasonality in Korean native goats. Nineteen primiparous goats of 2-years old and about 23㎏ of body weight were barn-housed at Gyeongsang National University Farm(N.L.: 35°1`) and allowed with daily supplements of about 200g and liberal feeding of grasses through the experimental period from November, 1982 to October, 1983. The goats were teased twice daily for 30 minutes in the morning(06:00) and evening(18:00) with two vasectomized bucks and behavioural estrus was recorded. For the effect of prostaglandin F₂α(PGF₂α) treatment on the induction of estrus, 10 anestrous goats were given intramuscular injection of 3㎎ PGF₂α(Lutalyse, Upjohn Co.) at a certain time of anestrus. Jugular vein blood was collected from venepuncture every 3 days from July 8 to August 7 for determination of serum progesterone concentration by radioimmunoassay. Eight of 10 anestrous goats were injected with PGF₂α in August. Two of 8 treated goats, Goat 29 and Goat 41 exhibited standing estrus in 12 and 24 hours after PGF₂α treatment. Serum progesterone concentration during anestrous period was ranged from 0.05 to 0.44 ng/㎖ except for a short period in 3 goats. Such levels of serum progesterone concentration are assumed much lower level compared to those during the luteal phase of estrous cycle of goats in breeding season. These data suggest that Korean native goats have a nonbreeding season of which duration may be varied among the individuals. The incidence of anestrous goats was found to be may frequent in summer than in any other season.
홍정의,이지삼,Hong, J.E.,Lee, J.S. 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.3
A total of 55 patients with various etiologies of infertility particitated in a study comparing two regimens of controlled ovarian hyperstimulation (COH) with GnRH agonists and gonadotropins. Nineteen patients were given an ultra-short stimulation protocol when the agonist was administered for 3 day from Day 2 of the cycle. The remaining 36 patients were given a long stimulation protocol when the agonist was administered from the mid-luteal phase of the cycle preceding the stimulation cycle. The mean number of gonadotropins used per patient was not different between two groups. No significant differences were found in the mean number of oocytes recovered, fertilization rate and embryo cleavage rate between two groups. Pregnancy and delivery rates were higher in ultra-short protocol than in long protocol, but these were not significant. These results suggest that an ultra-short protocol is as effective as a long protocol in in-vitro fertilization and embryo transfer.
산양의 수정란 이식 및 조작기법 개발에 관한 연구 Ⅰ. 산양의 발정유기 및 동기화에 관하여
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee) 한국축산학회 1989 한국축산학회지 Vol.31 No.1
This experiment was conducted to find out an efficient method for estrus induction and synchronization in goats. Sixty of 83 pluriparous Korean native goats received a single i.m. injection of 3㎎ PGF2α on Days 3 to 12 of the estrous cycle. Remained 23 goats received twice daily i.m. injection of 5㎎ progesterone for 6 or 7 days and a single i. m. injection of 3㎎ PGF2α 1 day before the last injection of progesterone. Serum progesterone concentrations were analyzed by RIA. A single intramuscular injection of 3㎎ prostaglandin F2α(PGE2α) on Days 5 to 12 of the estrous cycle induced estrus in 96% of 50 goats treated, but this treatment on Day 3 to 4 was not effective. Estrus was induced in all of 23 goats injected 3㎎ PGF 2αi.m. 24 hours before the last one of successive injections of 5mg progesterone for 6 or 7 days. The onset of estrus was significantly(P$lt;0.01) earlier in PGF 2α treated goats during Days 5 to 6 of the estrous cycle than those treated on Days 7 to 12. PGF 2α treatment with progesterone delayed significantly (P$lt;0.01) the onset of returning to estrus, compared with the PGF2α injection only during mid-luteal phase of the cycle. Serum progesterone concentration was decreased to a nadir at 24 hours following PGF2α administration on Days 5 to 8 of the cycle, but increased to 2.39±0.66 to 2.69±0.56ng / ml at 48 hours after PGF2α injection on Days 3 to 4. Incidence of short estrous cycle following estrus synchronization by PGF2α with or without progesterone was 26.1 % and 26.0 %, respectively. More effective synchronization of estrus was feasible by progesterone treatment for 6 or 7 days plus PGF2α injection 24 hours prior to the last progesterone administration, compared with a single intramuscular injection of 3㎎ PGF2α alone.
산양의 수정란 이식 및 조작기법 개발에 관한 연구 2 . 산양의 분만 및 유산 후의 단발정주기
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee) 한국축산학회 1991 한국축산학회지 Vol.33 No.4
This experiment was conducted to investigate the incidence of short estrous cycle and control of short cycle in Korean native goats. Abortion was induced by a single i.m. injection of 3 ㎎ PGF₂α at late stage of gestation. Does after parturition or abortion were teased twice daily with vasectornized bucks and behavioral estrus was recorded. Serum progesterone concentration was analysed by RIA. Mean post-partum or post-abortion interval to first estrus was shortest as 11.9 days in PGF₂α-aborted does compared to 22.1 days in does isolated from their kids within 7 days after parturition and 26.8 days in suckling does. Duration of this first standing estrus was not significantly different among groups. Four limes daily injections of 25 ㎍ Gn RH to does for Day 3 to 5 of the first estrous cycle following parturition or abortion ruduced significantly(p$lt;0.0l) the incidence of short estrous cycle to 38.5% compared to 80.0% in control group of does. Mean concentration of serum progesterone in does showing short estrous cycle was found to be basal level at estrual phase but considerably high level up to 1.06 ㎎/㎖ on Day 3 to 4 of the cycle. These results indicate that the short estrous. cycle may he resulted from the premature luteal regression and GnRH treatment during early luteal phase of the estrous cycle can partly prolong the short lifespan of corpora lutea.
산양의 수정란 이식 및 조작기법 개발에 관한 연구 3 . PMSG 와 FSH 를 이용한 산양의 과배란 유기
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee) 한국축산학회 1991 한국축산학회지 Vol.33 No.4
This study was conducted to investigate the effects of PMSG or FSH treatment with PGF₂α to induce superovulation in Korean native goats. Does were treated with a single s.c. injection of 1,000 IU PMSG on Day 12 or twice daily s.c. injections of a total of 20 ㎎ FSH in decreasing doses over 4 days beginning on Day 12 of the estrous cycle, Estrus was induced by a single i.m. injection of 10 ㎎ PGF₂α 48 hours after the first gonadotropins injection. The does in estrus were mated with intact bucks twice daily 12 hours apart. Ovarian responses and embryo development were observed by laparotomv 72 hours after mating. Mean interval from PGF₂α injection to onset of estrus was significantly(p$lt;0.01) shorter (45.2±7.5 hours) in FSH group than ill PMSG group(64.8±9.6 hours). Duration of estrus, length of estrous cycle and the incidence of short estrous cycle following superovulation were not significantly different between the 2 treatment groups. The mean number of corpora lutea(P$lt;0.01) and ma recovered(P$lt;0.05) were significantly greater for FSH group(13.2 and 9.2) than for PMSG group(5.1 and 3.5). The mean number of trans-ferable embryos kva, 5.9 and 1.9 in FSH-treated and PMSG-treated group. respectively. These results indicate that FSH treatment with PGF₂α could be superior to PMSG treatment with PGF₂α for the induction of superovulation in Korean native goats.
산양의 수정란 이식 및 조작기법 개발에 관한 연구 4 . 생쥐 및 산양 수정란의 분할조작에 의한 일란성 쌍태유기
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee),박희성(H . S . Park) 한국축산학회 1991 한국축산학회지 Vol.33 No.4
This experiment was conducted to establish a simple technique for bisecting mouse and goat embryos of morula to blastocyst stage and to develop a better culture and transfer system of demi-embryos for the production of identical twins. Mouse morulae or blastocysts and goat blastocysts were easily bisected without pronase treatment, using a microblade fixed to a micromanipulator. Bisected mouse embryos developing normally during culture were 71.4% of a total of 319 morulae and blastocysts. Survival rate of demi-embryos(29.0%) after culture and surgical transfer to recipient mice was comparable to result of intact embryos(26.0%). More transferable embryos (3.5±0.8/donor) were recovered in 7 to 8 days after mating when the does were induced to superovulate by injection of FSH-p in decreasing doses and progesterone priming for 10 days. and this superovulation regimen resulted in very low incidence of short estrous cycle(6.7%). Three kids were produced from 2 of 6 does received intact embryos. Fourteen pairs and one-half of demi-embryos were transferred to 15 recipients. of which one recipient delivered a single female kid.
산양의 수정란 이식 및 조작기법 개발에 관한 연구 5 . 생쥐 및 산양 분할 수정란의 생존성 및 이식 후 수태율의 향상
박충생(C . S . Park),최상용(S . Y . Choe),이효종(H . J . Lee),이지삼(J . S . Lee),박희성(H . S . Park) 한국축산학회 1991 한국축산학회지 Vol.33 No.5
This experiment was conducted to compare different procedures for improvement of viability of embryos after bisection and to investigate some factors affecting development of the bisected embryos. Demi-embryos were successfully produced by micromanipulation of mouse and goat embryos at stages from morula to blastocyst. In mouse, a superior viability(90%) of demi-embryos was achieved by culturing expanded blastocysts after bisection. The highest pregnancy rate(57.1%) and implantation rate(28.2%), which were almost same as the rates of intact embryos. were also achieved by transferring the demi-embryos at this stage after culture for 24 h in vitro. Twenty -eight 3%) of 30 embryos collected from superovulated Korean native goats were successfully bisected, of them 23 demi-pairs were transferred to recipients. Six and 3 does of them were diagnosed pregnant on 21 and 60 days of gestation, respectively. The present result indicates that in goat, hatched blastocysts might be more suitable for successful bisection to produce monozygotic twins than morulae or earlier blastocysts.