조화

이종헌 안암어문학회 1956 어문논집 Vol.1 No.1

Cyclosporin A Enhance Docetaxel Induced Apoptosis in Immortalized Human Oral Keratinocyte and Human Oral Squamous Cell Carcinoma Cell Line

이종헌,송영성,박경주 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.2

Oral squamous cell carcinoma (SCC) is one of the leading causes of cancer mortality worldwide. It is generally thought that adjuvant chemotherapy provides modest prolongation of survival in various carcinoma. Docetaxel (Taxotere, TXT) play a significant role in the treatment of various solid tumors of epithelial origin. CsA (immunosuppressive drug) was widely used as adjunct for the treatment of cancer. Thus, it is important to pursue the apoptosis of IHOK and oral SCC induced by TXT combined with CsA related to the pathogenesis of oral SCC. But TXT combined CsA effect on IHOK and oral SCC remains unclear. After cultured IHOK and HN 22 oral squamous cell carcinoma cell line treated by 10 nM TXT and 1 μM, and caspase inhobitor, respectively, apoptosis index, cytochrome c and caspase-3 -8, -9 mRNA expression by RT-PCR, and procaspase-3 protein amount by immunoslot blotting was prepared. The purpose of this study were to examine the TXT-induced apoptosis pathway via caspase activation by CsA enhancement, and to apply these results to an effective therapeutic treatment plan for oral SCC by TXT combined CsA . 10 nM TXT showed about 60%, 55% celluar apoptosis of IHOK and HN 22, cell line, respectively, while CsA alone did not induce apoptosis in IHOK and HN 22 cell line. 1 μM CsA combined with 10 nM TXT increased apoptosis in IHOK and HN 22 cell line through caspase-3 and cytochrome c mRNA expression, while could not effect on caspase-8 and -9. Caspase inhibitor suppressed apoptosis of IHOK and HN 22 cell line induced by a combination of 1 μM CsA and 10 nM TXT. Immnoslot blotting showed procaspase-3 activation by a combination 1 μM CsA and 10 nM TXT, while caspase inhibitor inhibited activation. It suggested that a combination of CsA and TXT might induce increased apoptosis of IHOK and HN 22 oral squamous cell carcinoma cell line through caspase-3 activation. This treatment with a combination of TXT and CsA may be an effective therapeutic strategy for oral squamous cell carcinoma.

비틀림을 이용한 후방압출에 관한 연구

이종헌,김진욱 한국기계기술학회 2002 한국기계기술학회지 Vol.4 No.2

Higher Expression of Urokinase - type Plasminogen Activator And Plasminogen Activator Inhibitor Type 1 in SGT Cell Line

이종헌,유상원,박경주 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.3

Urokinase-type plasminogen activator (uPA) and plasminogen activator type 1 (PAI-1) inhibitor contribute to the invasiveness of many carcinomas. It will be helpful to study clinical behavior of patients with malignant tumor by analysis of their expression. Expression of uPA and PAI-1 in human salivary gland tumors has been rarely reported in vitro. The purpose of this study were to investigate the protein expression of uPA and PAI-1 in SGT cell line compared to oral SCC and HeLa cell lines and to study migration and adhesion assay. All the cell lines were cultured under DMEM with 10% FBS at at 37oC in a 5% CO2 incubator. We studied a possible association between cytosolic uPA and PA-1 concentrations in SGT cell line compared to any other cell lines through cell migration and adhesion assay, and enzyme-linked immunoassay(ELISA). In migration assay SGT cell line was about 2 .5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPA cytosolic concentrations of SGT cell line was about 3-10 folds, while PAI-1 was about 2.5-10 folds. Oral SCC cell lines were the lowest value. Both uPA and PAI-1 concentrations were correlated with migration and adhesion assay. High cytosolic concentrations of uPA and PAI-1 was correlated with migration and adhesion assay. It suggested that these markers might be specific marker for SGT cell line and would be contributed to treatment and prognosis of human salivary gland adenocarcinoma.

Transgluraminase 2 Expression Was Associated with Nuclear Factor Kappa in Salivary Gland Tumor Cell Line

이종헌,김인수,박경주 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.2

The transcription factor nuclear factor-kB (NF-kB) plays an important role in regulating cell growth, apoptosis,and metastatic functions. Constitutive activation of NF-KB has been observed in various cancers; however, molecular mechanisms resulting in such activation remain elusive. Numerous evidences showed that over expression of TGase 2 might be linked with constitutive activation of NF-KB. To understand the pathways responsible for constitutive activation of NF-kB is important for rational design of NF-kB inhibitors for cancer therapy. Human salivary gland adenocarcinoma has been most aggressive solid tumors. Current therapy does not significantly improve survival rates. Thus, to investigate new therapeutic modalities against this adenocarcinoma is necessary. The purpose of this study was to study a constitutive activation of NF-kB with the expression of TG2 in SGT cell line origianted from human salivary gland adenocarcinoma by TGase 2 activity, RT-PCR and immunoslot blotting method. SGT cell line showed the highest TGase 2 activity and NF-kB activation than any other cell line. All the cell lines showed increased NF-kB mRNA activation after A231027 treatment than that of control. In immunoslot blotting,SGT cell line showed NF-kB activation correlated with TGase 2 expression after A231027 and BSA. It suggested that there might be a direct correlation between TGase 2 expression and NF-kB activation in SGT cell line.

북한 핵문제의 경제학적 고찰

이종헌 한국국방연구원 2008 국방정책연구 Vol.23 No.4

This paper is aimed at exploring motives behind North Korea’s nuclear aspirations by employing an economic framework of analytical tools. Applying the concepts of utility and efficiency helps understand puzzling political behaviors. The rational choice model provides an effective tool for understanding North Korea’s behaviors, which appeared irrational, unreasonable and often mysterious, as shown the choice of nuclear weapons. Behavioral economics, a sub-field combining psychology and economics, can also be fruitfully applied to explain behaviors that hardly be understood as rational. This research examines North Korea’s nuclear choice from these two points of view. From rational choice terms which assumes leaders are self-interested and calculate costs and benefits of alternative course of actions that maximize their utility, North Korea’s nuclear option is the outcome of the process of goal-setting, recognition of circumstances and choice of the best option to achieve the goal of survival in the face of threats from the outside. This research analyzes the country's “goal”, “recognition” and “choice” under the rational choice framework. This paper also uses behavioral economics approach, which shows that North Korea’s decision to develope nuclear weapons might be motivated by the leader’s prompt emotions, such as flash antagonism against foreign enemies rather than rational calculation.

삼국지 명구선 ( 2 ) : 역사란 분열과 통합의 연속 - 천하대세 분구필합 합구필분 -

이종헌 전국한자교육추진총연합회 2002 한글한자문화 Vol.33 No.-

북한의 정책선택 패턴: 생존성과의 상관관계

이종헌 통일연구원 2007 統一 政策 硏究 Vol.16 No.1

North Koreas Policy Choice Patern: Focused on Its SurvivabilityThis research explores strategic di-mensions of the North Korean rulers choices on how to produce power and obtain public loy-alty in a framework of rational choice. The po-litical economic analysis of the survivability of the North Korean regime is centered on its stra-tegic use of cooption, repression and its unique strategy of deification.Cooption of people into the ruling elite and repression is a stick to ensure loyalty through a threat or use of violence. Deification is a long-term strategy to obtain loyalty. The police choices were reflected by the countrys budget expenditures.The three elements are neither a sudden fallout or nor a passing phenomenon, but strat-egies chosen by its leaders to maintain the re-gime’s survivability. The regime has also stra-tegically adjusted the extent of cooption and re-pression to boost its survivability.By and large, North Korea employed a combination of more cooption and less re-demand for tougher repression which is a dan-gerous option that can trigger a backlash. This policy combination has helped North Korea survive despite global collapse of communism and economic meltdown. The North Korean behaviors follow patterns which can be under-stood in rational-choice terms.

The Inhibitory Effect of Cyclosporine A on Plaminogen Activtor in Immortalized Human Oral Keratinocyte

이종헌 대한구강악안면병리학회 2011 대한구강악안면병리학회지 Vol.35 No.4

Plasminogen activators(PA) such as urokinase(uPA) and tissue type plasminogen activators(tPA), physiologically catalyze the conversion of the plasminogen to the wide spectrum proteinase plasmin. Because uPA and tPA are involved in cell growth, differentiation and migration of oral cancer, oral epithelial carcinogenesis including transformation of precancerous lesion into oral squamous cell carcinoma with PA is very interesting. It is important to prevent precancerous condition from transfoming into oral squamous cell carcinoma by the inhibitory effect of various drug. It is well known that cyclosporine A(CsA) as immunosuppressive properties exerts anti-cancer effects. Recently it is widely accepted that cultured immortalized oral keratinocyte (IHOK) is considered as an intermediate stage of oral carcinogenesis and used as precancerous condition in vitro. Thus it was thought that it might be interesting to investigate CsA effect on PA expression of IHOK. IHOK was cultured under KBM bullet kit at 37℃ under 95% CO2 incubator. Subconfluent IHOK cells was treated at different CsA concentration. uPA and tPA protein expression from cultured IHOK cell line has been detected by ELISA analysis in the CsA-treated samples. uPA expression of IHOK was higher than that of NHOK, while tPA was similar to that of NHOK. After CsA treatment, CsA might not effect the expression of uPA of IHOK, while showed a little effect on tPA of IHOK. It suggested that CsA had no effect in uPA expression of IHOK although uPA could be used as a marker for precancerous lesion.

Modification of high-density polyethylene with poly(n-butyl acrylate) segments through melt process using controlled/“living” radical polymerization technique

이종헌,홍성철 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.1