흰쥐의 간세포에서 호르몬에 의한 Acetyl-CoA Carboxylase Promoter I Activity 조절에 대한 연구

이막순(Lee Mak Soon),양정례(Yang Jeong-Lye),김윤정(Kim Youn Jung),김영화(Kim Young Hwa),김양하(Kim Yang-Ha) 한국영양학회 2002 Journal of Nutrition and Health Vol.35 No.2

Isorhamnetin의 근육세포 미토콘드리아 기능조절에 미치는 효과

이막순(Mak-Soon Lee),김양하(Yangha Kim) 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.4

Purpose: Muscle mitochondria play a key role in regulating fatty acid and glucose metabolism. Dysfunction of muscle mitochondria is associated with metabolic diseases such as obesity and type 2 diabetes. Isorhamnetin (ISOR), also known as 3-O-methylquercetin, a quercetin metabolite, is a naturally occurring flavonoid in many plants. This study evaluated the effects of ISOR on the regulation of the mitochondrial function of C2C12 muscle cells. Methods: C2C12 muscle cells were differentiated for 5 days, and then treated in various concentrations of ISOR. Cytotoxicity was determined by assessing cell viability using the water-soluble tetrazolium salt-8 assay principle at different concentrations of ISOR and time points. Levels of the mitochondrial DNA (mtDNA) content and gene expression were measured by quantitative real-time polymerase chain reaction. The citrate synthase (CS) activity was quantified by the enzymatic method. Results: ISOR at a concentration of 10 μM did not show any cytotoxic effects. ISOR increased the mtDNA copy number in a time- or dose-dependent manner. The messenger RNA levels of genes involved in mitochondrial function, such as peroxisome proliferator-activated receptor-γ coactivator-1α, and uncoupling protein 3 were significantly stimulated by the ISOR treatment. The CS activity was also significantly increased in a time- or dose-dependent manner. Conclusion: These results suggest that ISOR enhances the regulation of mitochondrial function, which was at least partially mediated via the stimulation of the mtDNA replication, mitochondrial gene expression, and CS activity in C2C12 muscle cells. Therefore, ISOR may be useful as a potential food ingredient to prevent metabolic diseases-associated muscle mitochondrial dysfunction.

마치현 추출물이 3T3-L1 지방세포에서 지방 분해 및 Hormone Sensitive Lipase (HSL) 유전자 발현에 미치는 효과

이막순(Lee Mak Soon),김종태(Kim Chong Tai),김철진(Kim Chul-Jin),조용진(Cho Young-Jin),김양하(Kim Yangha) 韓國營養學會 2006 Journal of Nutrition and Health Vol.39 No.8

PGC-1α 형질전환 생쥐에서 마늘 분말의 체지방 감소 효과

이막순 ( Mak-soon Lee ),김양하 ( Yangha Kim ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.5

This study was performed to investigate the body fat-lowering effect of garlic powder in peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1α)-luciferase transgenic mice (TG). In this study, we generated transgenic mice with a PGC-1α promoter (―970/+412 bp) containing luciferase as a reporter gene. Mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (CON), or high-fat diets supplemented with 2% (GP2) or 5% (GP5) garlic powder for an additional 8 weeks. Dietary garlic powder reduced the body weight in the GP2 and GP5 groups, compared to the CON group. Furthermore, garlic supplementation significantly decreased the plasma levels of triglycerides, total cholesterol, and leptin in the GP5 group, compared to the CON group. Specifically, luciferase activity in liver, white adipose tissue (WAT), and brown adipose tissue (BAT) was increased by garlic supplementation in a dose-dependent manner. These results suggest that the body fat-lowering effect of garlic powder might be related to PGC-1α by the increase in luciferase activity in liver, WAT, and BAT. Furthermore, transgenic mice might be useful for evaluating the body fat-lowering effect of various health functional foods.

뽕나무 열매 착즙 분말이 3T3-L1 지방세포의 염증 및 microRNA-132/143 조절에 미치는 영향

이막순(Mak-Soon Lee),김양하(Yangha Kim) 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.5

본 연구는 뽕나무 열매 착즙 분말 (MJ)이 지방세포 분화과정에서 염증에 관여하는 유전자 발현 및 miR-132/143 조절에 미치는 효과에 중점을 두고 조사하였다. MJ는 3T3-L1 지방세포 분화 동안 지질축적이 억제되었고 PPAR-γ, CEBP-α 및 aP2와 같은 지방형성 유전자 발현을 억제하였다. 또한 염증 조절 매개체인 TNF-α, IL-6, MCP-1 및 iNOS 유전자 발현이 MJ 처리에 의해 감소되었다. MJ의 지질 축적 억제 효과는 염증과 지방분화에 관여하는 miR-132/143의 발현 감소와 관련이 있음을 확인하였다. 따라서 MJ는 염증을 동반하는 비만 예방 및 치료에 도움이 될 수 있는 식품 소재로서 가능성이 있음을 시사하고 있다. 그러나 MJ의 주요 성분인 안토시아닌의 생체이용율은 1–2% 미만으로 추정되며 예상되는 표적 조직이나 혈류에서 미량 검출되는 것으로 알려져 있다. 이를 해결하기 위해서는 생체 내 동물실험을 통한 다각적인 분석이 향후 진행되어야 할 것으로 판단된다. Purpose: Mulberry (Morus alba L.) fruit is widely grown in Asia and consumed as fresh fruit, jam, and juices. The fruit has beneficial health effects, including anti-diabetic, anti-tumor, and anti-obesity properties. However, the mechanisms by which mulberry fruit juice powder (MJ) regulates inflammatory microRNAs (miRs) are not yet known. This study investigated the effect of mulberry fruit juice powder on the regulation of inflammation and miR-132/143 during 3T3-L1 adipocyte differentiation. Methods: The 3T3-L1 cells were induced to differentiate for 2 days and then treated with various concentrations of MJ for 7 days. Cytotoxicity was determined by evaluating cell viability using a water-soluble tetrazolium salt-8 assay kit. Intracellular lipid accumulation was evaluated by oil-red O staining. The levels of the expression of genes involved in adipogenesis and inflammation, and miR-132/143 were measured by quantitative real-time polymerase chain reactions. Results: MJ showed no cytotoxic effect on 3T3-L1 adipocytes at concentrations below 100 ng/mL. Intracellular lipid accumulation was reduced by MJ treatment at concentrations of 100 ng/mL. The messenger RNA (mRNA) levels of proliferator-activated receptor-γ, cytosinecytosine-adenosine-adenosine-thymidine/enhancer-binding protein-α, and adipocyte protein 2, which are involved in adipogenesis, were suppressed by MJ. A reduction was also seen in mRNA levels of genes related to the inflammatory response, such as tumor necrosis factor-α, interleukin-6, monocyte chemoattractant protein-1, and inducible nitric oxide synthase. The expression of the inflammatory miR-132 and miR-143 was also decreased by MJ. Conclusion: These results suggest that MJ may suppress adipogenesis and inflammation through the regulation of miR-132/143 expression in 3T3-L1 adipocytes. Thus, MJ may be useful as a food agent that prevents obesity-associated inflammation.

마늘이 고지방 식이를 섭취한 UCP2 형질전환 마우스의 대사성 조직에서 UCP2 전사 조절에 미치는 영향

이막순 ( Mak-soon Lee ),이서현 ( Seohyun Lee ),신윤진 ( Yoonjin Shin ),정선윤 ( Sunyoon Jung ),박선영 ( Seonyoung Park ),김양하 ( Yangha Kim ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.3

This study was performed to investigate the effects of garlic on uncoupling protein 2 (UCP2) transcriptional regulation of UCP2- luciferase transgenic mice fed on a high fat diet to induce obesity. To examine the transcriptional regulation of UCP2, we generated transgenic mice with a UCP2 promoter (-1,830/+30 bp) containing luciferase as a reporter gene. UCP2-luciferase transgenic mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (TG-CON), or high-fat diets supplemented with 2% (TG-GL2) or 5% (TG-GL5) garlic for a further 8 weeks. Dietary garlic reduced body weight and energy efficiency ratio in the TG-GL5 group, compared to the TG-CON group. Furthermore, garlic supplementation significantly decreased white adipose tissue fat mass and plasma levels of triglycerides, total cholesterol, and leptin in the TG-GL2 and TG-GL5 groups, compared to the TG-CON group. Specifically, UCP2 promoter activity in metabolic tissues such as liver, white adipose tissue, brown adipose tissue, and skeletal muscle was increased by garlic supplementation. These results suggest that dietary garlic was partially associated with an increase of UCP2 transcriptional activity in metabolic tissues for decreasing obesity.

닭고기 저장 동안 천연첨가물에 의한 병원성 미생물 억제 효과

이막순(Mak-Soon Lee),김승혜(Seunghae Kim),문소희(Sohee Moon),김양하(Yangha Kim) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.5

본 연구의 목적은 닭고기의 저장 기간 동안 천연 첨가물에 의한 병원성 미생물 생장 억제 효과를 조사하기 위함이다. 닭고기 저장기간 동안 천연 첨가물에 의한 병원성 미생물 억제 효과를 조사하기 위하여 닭고기의 껍질 표면에 천연 첨가물을 단독 혹은 배합하여 처리하였다. 병원성 미생물은 닭고기 냉장 저장기간 중에 위생과 안전성에 영향을 미치는 부패세균인 S. Typhimurium 및 P. aeruginosa를 사용하였다. 천연첨가물은 키토산, EGCG, 마늘을 각각 2% 농도로 시료 표면에 처리한 다음 4℃ 냉장 조건하에서 12일 동안 6일 간격으로 2번에 걸쳐 병원성 미생물 억제효과를 관찰하였다. 각각의 병원성 미생물의 생장 억제 효과는 키토산, EGCG 및 마늘을 단독 혹은 두 가지 배합 처리한 것보다 세 가지 배합 처리한 것이 처리 시간별로 훨씬 더 높은 미생물 억제 효과를 나타내었다. 이 결과들은 키토산, EGCG와 마늘을 포함하는 세 가지 배합된 천연첨가물이 닭고기의 저장 동안 병원성 세균 생장 억제를 위한 천연첨가제로 유용하게 사용될 수 있음을 시사하고 있다. 아울러 본 연구 결과들은 천연첨가물을 이용한 유해 미생물 제어 기술 개발을 위한 기초 자료로 사용될 수 있을 것으로 기대된다. The purpose of this study was to examine the antimicrobial effects of natural additives on pathogenic microorganisms in commercial chicken during storage. Chicken skin (20 cm²) inoculated with Salmonella Typhimurium or Pseudomonas aeruginosa was treated with 2% natural additives, including chitosan, epigallocatechin gallate (EGCG) and garlic, during storage at 4℃ for 12 days. A combination of the three natural additives significantly inhibited growth of S. Typhimurium compared to treatment with chitosan, EGCG or garlic alone (p<0.05). This combination also inhibited growth of P. aeruginosa compared to treatment with one or two of the natural additives (p<0.05). These results suggest that combined chitosan, EGCG, and garlic may be used as an antimicrobial agent in commercial chicken during refrigerated storage.

고콜레스테롤 식이 투여 흰쥐에서 옥수수 펩타이드 섭취가 혈중 지질 성상에 미치는 효과

문민선(Moon Min-Sun),이지혜(Lee Ji Hye),이막순(Lee Mak Soon),권인숙(Kwun In Sook),정용일(Chung Yang Il),이대희(Lee Dae Hee),김양하(Kim Yangha) 韓國營養學會 2006 Journal of Nutrition and Health Vol.39 No.8