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상용 휘발유로부터 분리한 다환 방향족 탄화수소(PAH) 분해 세균의 특성
권태형 ( Taehyung Kwon ),우정희 ( Junghee Woo ),박년호 ( Nyunho Park ),김종식 ( Jongshik Kim ) 한국환경농학회 2015 한국환경농학회지 Vol.34 No.3
BACKGROUND: Recent studies have described the importance of bacteria that can degrade polycyclic aromatic hydrocarbons (PAHs). Here we screened bacterial isolates from commercial gasoline for PAH degraders and characterized their ability to degrade PAHs, lipids and proteins as well as their enantioselective epoxide hydrolase activity, salt tolerance, and seawater survival. METHODS AND RESULTS: One hundred two bacteria isolates from commercial gasoline were screened for PAH degraders by adding selected PAHs on to the surface of agar plates by the sublimation method. A clear zone was found only around the colonies of PAH degraders, which accounted for 13 isolates. These were identified as belonging to Bacillus sp., Brevibacterium sp., Micrococcus sp., Corynebacterium sp., Arthrobacter sp., and Gordonia sp. based on 16S rRNA sequences. Six isolates belonging to Corynebacterium sp., 3 of Micrococcus sp., Arthrobacter sp. S49, and Gordonia sp. H37 were lipid degraders. Arthrobacter sp. S49 was the only isolate showing high proteolytic activity. Among the PAH-degrading bacteria, Arthrobacter sp. S49, Brevibacterium sp. S47, Corynebacterium sp. SK20, and Gordonia sp. H37 showed enantioselective epoxide hydrolase activity with biocatalytic resolution of racemic styrene oxide. Among these, highest enantioselective hydrolysis activity was seen in Gordonia sp. H37. An intrinsic resistance to kanamycin was observed in most of the isolates and Corynebacterium sp. SK20 showed resistance to additional antibiotics such as tetracycline, ampicillin, and penicillin. CONCLUSION: Of the 13 PAH-degraders isolated from commercial gasoline, Arthrobacter sp. S49 showed the highest lipid and protein degrading activity along with high active epoxide hydrolase activity, which was the highest in Gordonia sp. H37. Our results suggest that bacteria from commercial gasoline may have the potential to degrade PAHs, lipids, and proteins, and may possess enantioselective epoxide hydrolase activity, high salt tolerance, and growth potential in seawater.
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아버지의 결혼만족도 및 양육태도와 유아의 사회적 능력간의 관계
우정희,이미숙,전춘애 대한가정학회 2003 Family and Environment Research Vol.41 No.12
This study investigated the relationship between fathers marital satisfaction, child rearing attitude and children s social competence. The subjects of this study were 274 fathers of 4 - 5 year old children who enrolled in kindergartens located in Seoul and metropolitan areas. Data was analyzed using the following methods: frequency, percentage, mean, Pearson s correlation andpis, multiple regression Mi and Cronbach's a. As a result of thii study, it was revealed that there were a significant relationship between the marital satisfaction and child rearing attitude of fathers and the social competence of children. However, this study showed that the child rearing attitude rather than the marital satisfaction of fathers was the more influential on the social competence of children.
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