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김창근,이용빈,오연각 한국축산학회 1969 한국축산학회지 Vol.11 No.2
This experiment was carried out to study the effect of the rat and bull spermatozoa motility on the transport and leucocytic response in the genital tract o the estrous rat. Further study was also made to investigate the effect of the etsrogen and progesterone on the infiltration of leucocyte in the ovariectomized rat. Each rat was injected with motile, nonmotile rat or motile bull spermatozoa into the lower part of the right uterine horn of the normal estrous rat after laparotomy and motile rat spermatozoa to they hormone-treated rat. In this study 60 mature female albino rats were used. Time of heat was determined by vaginal smear and then confirmed by vasoectomized male. At 1, 8 or 16 hrs. after injection of the semen, the number of spermatozoa and leucocytes in various segments of the tract was counted and compared with each treatment. The results obtained were as follows: 1) The distribution of spermatozoa in the right uterus at 1 hr. after injection was not significantly different among the three treatments and the segments investigated, but more spermatozoa were found in Lower part than upper part at 8 hrs. in all treatments (P$lt;0.05). At 8 and 16 hrs. the number of spermatozoa was diminished greatly more than hr., which w-as caused by evacuation of the uterine contents into the vagina. The number of evacuated spermatozoa into the vaging was significantly appeared at 8 hrs. as compared with I hr. or 16 hrs. (P$lt;0.05). 2) The total number of spermatozoa reached in the righi Fallopian tube was recovered much more in motile rat spermatozoa than the others and showed also in the ampulla, site of fertilization. Even though the small number of spermatozoa was recovered is the Fallopian tube, the nonmotile rat and motile bull spermatozoa can pass through the utero-tubal junction. However, it was not utterly found in the site of fertilization. 3) The leucocytic response in normal estrous rat was not significantly affected by spermatozoan motility of the rat and bulk. The number of leacocytes in various segments of the genital tract was statistcally significant less in the vagina than sipper part and lower part of the uterus (P$lt;0.01 and P$lt;0.05, resp.), but no significant difference was found between upper and lower part. The difference of leucocytic response was not observed significantly between 8 and 16 hrs., but highly significant difference was observed between 1 hr. and the others. 4) In the ovarian hormone-treated rat after ovariectomy, the leucocytic response was observed significantly different among the treatments (P$lt;0.05) and the intervals investigated(P$lt;0.01). At 1 hr. after injection the difference in the leucocyte number was not significant among the three treatments, but at 8 hrs. (P$lt;0.05) and 16 hrs. (P$lt;0.01) the number of spermatozoa in the progesterone-treated rat was observed significantly less than in the other rat and there were no significant difference among the intervals. However, in the estrogen-treated rat the more spermaozoa were appeared in the genital tract as the time passed after injection.
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토끼의 수정란 이식에 있어서 난세포 분열기와 이식부위에 관한 연구
김중계,서국성,신원집,오연각,설동섭,김상철,이용빈 ( J . K . Kim,G . S . Suh,W . J . Shin,Y . K . Oh,D . S . Sul,S . C . Kim,Y . B . Lee ) 한국축산학회 1974 한국축산학회지 Vol.16 No.1
This experiment was carried out to study a suitable method of fertilized ova transfer in the rabbit. A total of 160 ova at 2 to 16 cell stages or at 16 cell to morula stages from superovulated donors were transferred at the rate of 8 per animal to the fallopian tubes and uterus of 20 recipient females, mated 24 hrs. earlier to vasectomised male. Irrespective of the site of transfer, the proportion of ova developing to term following 16 cell to morula stage transfers was more than that following 2-to 16 cell stage transfers. And uterus transfers were more successful than fallopian transfers in both 2 to 16 cell stages and 16 cell to morula stages. The results with 16 cell to morula stage transfers at the uterus were better than those in any other groups. Fourteen of 20 recipients became pregnant, of which il produced a total of 32 offsprings, and litter size averaged 2.9 with variation from 1 to 6.
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김창근 ( C K Kim ),김상철 ( S C Kim ),이용빈 ( Y B Lee ),설동섭 ( D S Sul ),오연각 ( Y K Oh ),서국성 ( G S Suh ),김중계 ( J K Kim ) 한국축산학회 1972 한국축산학회지 Vol.14 No.2
The ovulation rate and follicle development were observed in 18 goats by laparotomy on the third day after injection of 500, 750 or 1,000 I.U. PMS. Fecundity was also observed in other 17 goats treated with 500, 750 or 1,000 I.U. PMS and mated naturally on the 17th day of the estrous cycle. The ovulation rate was 1.5 (1∼3), 2.5 (1∼4) and 3.2 (2∼5) in the goats receiving 500, 750 or 1,000 I.U. PMS, respectively, and it was found significantly (P$lt;0.05) mare in 1,000 I.U. PMS than in 500 I.U. PMS. However, there were no significant differences in the number of follicles with 5mm or larger diameter and hemorrhagic follicles between the groups. Fecundity was 2.0, 2.25 and 2.0 in the goats receiving 500, 750 and 1,000 I.U. PMS, respectively. The single, twin, triplet and quadruplet births were 23.5%, 52.7%, 17.7% and 5.9%, respectively. One still birth and 2 premature births were observed. The shorter gestation period of goats and the lighter birth weight of kids resulted from the higher fecundity.
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