Comparison of Clinical Outcomes of Same-size Grafting between Deep Anterior Lamellar Keratoplasty and Penetrating Keratoplasty for Keratoconus

오백록,김미금,위원량 대한안과학회 2013 Korean Journal of Ophthalmology Vol.27 No.5

Purpose: To compare the clinical outcomes between deep anterior lamellar keratoplasty (DALK) and penetrating keratoplasty (PKP) with same-size grafts in patients with keratoconus. Methods: Medical records of 16 eyes from 15 patients treated from June 2005 through April 2011 were retrospectively reviewed. Patients with contact lens intolerance or who were poor candidates for contact lens fitting due to advanced cone underwent keratoplasty. The transplantations consisted of 11 DALK and 5 PKP with same-size grafting for keratoconus. Best-corrected visual acuity (BCVA), refractive error, corneal topographic profiling, and clinical course were compared between DALK and PKP groups. Results: The follow-up period was 30 ± 17 months in the DALK group and 45 ± 20 months in the PKP group (p = 0.145). At final follow-up, the DALK and PKP groups achieved a BCVA (logarithm of the minimum angle of resolution) of 0.34 and 0.52, respectively (p = 0.980). Postoperative refractive error and mean simulated keratometric index showed myopic astigmatism in both groups without any statistical difference. Corneal irregularity index measured at 5 mm in the DALK group was less than that of the PKP group at 1-year followup (p = 0.021); however, at final follow-up, there was no longer a statistically significant difference. Endothelial cell counts were lower in the PKP group than in the DALK group at final follow-up (p = 0.021). Conclusions: The optical outcomes of DALK with same-size grafts for keratoconus are comparable to those of PKP. Endothelial cell counts are more stable in DALK compared to PKP.

Optimization of Culture Conditions for 1,3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae Using Response Surface Methodology

오백록,서정우,최민호,김철호 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.6

To produce 1,3-propanediol (1,3-PD) from crude glycerol, cultivation conditions were optimized by response surface methodology (RSM) based on a 25 factorial central composite design (CCD). RSM was adopted to derive a statistical model for the individual and interactive effects of crude glycerol, (NH₄)₂SO₄, pH, cultivation time and temperature on the production of 1,3-PD. Optimal conditions for maximum 1,3-PD production were as follows: crude glycerol, 35 g/L; (NH₄)₂SO₄, 8 g/L; pH, 7.37; cultivation time, 10.8 h; temperature, 36.88℃. Under these optimal conditions, the design expert presented the maximal numerical solution with a predicted 1,3-PD production level of up to 13.74 g/L. The experimental production of 1,3-PD yielded 13.8 g/L, which was in close agreement with the model prediction.

공막연화증에서 시행한 양막이식술에서 재이식의 위험인자에 대한 장기 관찰 분석

오백록(Baek Lok Oh),김미금(Mee Kum Kim),최혁진(Hyuk Jin Choi),위원량(Won Ryang Wee) 대한검안학회 2012 Annals of optometry and contact lens Vol.11 No.1

목적: 익상편절제술 후 발생한 공막연화증으로, 상피결손과 공막결손을 복구하기 위해 양막이식술을 시행받았으나, 양막 재이식이 필요했던 환자에서 재이식의 위험인자에 대해 분석하고자 하였다. 대상과 방법: 양막 이식 수술 후 6개월 이상 추적관찰하였던 14명 15안의 의무기록 후향적 분석을 하였다. 수술 후 재수술이 필요했던 4안과 그렇지 않았던 11안에서, 환자의 성별, 나이, 익상편절제술부터 공막연화증 발생까지 걸린 기간, 수술 당시의 염증정도, 임박한 천공유무, 칼슘판 유무, 미생물검사결과, 공막이식술 실시여부, 수술 후 자가혈청 사용여부, 수술 중 측정한 공막결손의 크기를 비교하였다. 결과: 동시 공막편 이식여부 및 기타 위험인자는 두 그룹에서 유의한 차이를 보이지 않았으며(p=0.538), 수술 중 측정한 공막결손의 크기만 두군에서 유의하게 차이를 보였다(p=0.012). 공막편이식을 하지 않은 양막이식만 시행한 소집단분석시에도 공막결손 크기 외에 위험인자들은 유의한 차이를 보이지 않았다. 결론: 공막연화증에서 공막편 이식여부는 재이식에 영향을 끼치지 않으며, 수술 중 측정한 공막결손의 크기는 양막이식술 후 재이식의 위험인자로 추정된다. Purpose: To investigate the risk factors of re-transplantation of amniotic membrane to repair epithelial and scleral defect in scleromalacia after pterygium excision. Methods: The medical records of fifteen eyes in 14 patients who had followed-up at least 6 months after amniotic membrane transplantation were retrospectively reviewed. We compared presumable risk factors of re-transplantation group (n=4) to those of control group (n=11), such as sex, age, onset of scleromalacia after pterygium excision, activity of scleritis at the time of operation, impending perforation, existence of calcium plaque, microbe culture result, the size and area of sclera defect, simultaneous application of scleral patch graft, postoperative use of autoserum. Results: There was no statistical significance in the concomitant application of scleral patch graft (p=0.536) and other risk factors between two groups except scleral defect size (p=0.012). The only significant risk factor was scleral defect size in sub-group analysis which had undergone amniotic transplantation only without scleral patch graft. Conclusions: Scleral patch graft might not be involved in the risk of re-transplantation, of amniotic membrane, while the size of scleral defect might be the risk factor to predict amniotic membrane re-transplantation after repair of scleromalacia.

항암 치료 후 발생한 빈혈망막병증

이승찬,오백록 대한안과학회 2019 대한안과학회지 Vol.60 No.12

목적: 항암 치료 후 발생한 빈혈망막병증 1예를 보고하고자 한다. 증례요약: 32세 남자 환자가 3일 전부터 시작된 시야이상을 호소하며 내원하였다. 내원 4개월 전 우측 고환암에 대해 고환절제술을 시행받았으며 이후 보조항암요법을 진행하여 2주 전에 치료를 마쳤다. 내원시 양안 경도의 시력저하를 보였으며, 안저검진상 양안후극부에 다발성 화염상 출혈과 함께 황반부 배 모양 출혈이 관찰되었다. 혈액검사상 중증 빈혈(혈색소 5.5 g/dL)과 함께 경도의 혈소판감소증(77,000/μL)이 확인되었고 이를 바탕으로 양안의 빈혈성 망막병증으로 진단하였다. 적혈구 및 혈소판 수혈 후 혈액검사수치는 거의 정상화되었으며, 이와 함께 시력호전 및 안저검진상 망막출혈이 소실되는 것을 확인할 수 있었다. 결론: 양안에 망막출혈을 일으킬 수 있는 여러 질환들은 안저 소견만으로 명확하게 감별하기 힘든 경우가 많다. 주의 깊은 안저 검진뿐 아니라 환자의 병력, 동반되는 전신증상 및 검사실 소견 등을 자세히 파악하는 것 역시 중요하다. Purpose: To report a case of anemic retinopathy after chemotherapy. Case summary: A 32-year-old male presented with visual disturbance in both eyes. He had been diagnosed with testicular cancer and had undergone right orchiectomy 4 months prior. He completed adjuvant chemotherapy 2 weeks before presentation. His best-corrected visual acuities were 20/35 in both eyes. Fundus examination revealed multiple flame-shaped hemorrhages around the posterior pole, and boat-shaped hemorrhages on the macula in both eyes. Laboratory results showed that he had reduced hemoglobin (5.5 g/dL) and platelet counts (77,000/μL). After transfusion, visual acuities were improved and retinal hemorrhages were resolved along with normalization of the hematological conditions. Conclusions: Many ocular and medical conditions can cause bilateral retinal hemorrhages. This case emphasizes that comprehensive history evaluation, systemic evaluation, and laboratory findings, as well as a detailed fundus examination, are important in the diagnosis of patients with bilateral retinal hemorrhages.

Production of Lipid Containing High Levels of Docosahexaenoic Acid by Cultivation of Aurantiochytrium sp. KRS101 Using Jerusalem Artichoke Extract

주정현,오백록,류승규,허선연,김수연,홍원경,김철호,서정우 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.6

In the present study, we evaluated extract of Jerusalem artichoke tubers (JAT) as a substrate for the production of lipid containing high levels of docosahexaenoic acid (DHA) by cultivated Aurantiochytrium sp. KRS101, an oleaginous protist. The optimal conditions for cultivation determined using response surface methods were as follows: pH, 5.9; enzyme loading, 282.6; and temperature, 27.7°C. The maximal levels of lipid (16.4 g/L) and productivity (3.6 g/L/d) were obtained during fed-batch fermentation, which achieved a DHA yield of 7.9 g/L (>48% of total fatty acids by weight). Moreover, JAT was more economical, reducing costs of expensive yeast extract as a nitrogen source by about 40%. These results suggest that JAT may be further explored as a low-cost feedstock for lipid production using microalgae strains.

분자유전검사로 확진된 상염색체 우성 드루젠

송재신,오백록,박운철,유형곤,이은경 대한안과학회 2021 대한안과학회지 Vol.62 No.1

Purpose: To report a case of autosomal dominant drusen confirmed by molecular genetic testing and the clinical features and findings of a multimodal diagnostic imaging study. Case summary: A 32-year-old male presented with decreased visual acuity in his right eye from 1 year prior to his first visit. On the first visit, his visual acuities were 0.3 in the right eye and 1.2 in the left eye. A fundus examination showed numerous drusen of various sizes widely distributed on the posterior pole of both eyes, as well as subretinal fibrotic change with pigmentation in the right eye. Optical coherence tomography showed extensive hyperreflective deposits beneath the retinal pigment epithelium in both eyes and small amounts of cystic intraretinal fluid in the right eye. Fluorescein angiography and fundus autofluorescence showed the drusen as multiple hyperfluorescent spots, while indocyanine green angiography indicated hypofluorescence corresponding to the drusen. Genetic sequence analysis revealed a pathogenic variant of the EFEMP1 gene, heterozygous c.1033C>T (p.Arg345Trp), which accords with the diagnosis of autosomal dominant drusen. Intravitreal bevacizumab injection was given in the right eye; however, there was no improvement in the amount of intraretinal fluid nor visual acuity. Conclusions: Autosomal dominant drusen is a very rare disease. It is necessary to distinguish it from age-related macular degeneration, as the affected age and clinical features of drusen are different, including the response to anti-vascular endothelial growth factor treatment. 목적: 분자유전검사로 확진된 상염색체 우성 드루젠 1예를 경험하여 이의 임상적 특징 및 여러 진단적 이미지 검사 소견들을 보고하고자 한다. 증례요약: 32세 남성이 1년 전부터 시작된 우안 시력저하를 주소로 내원하였다. 나안시력 우안 0.3, 좌안 1.2였고, 안저검사상 양안 후극부에 광범위하게 분포한 다양한 크기의 수많은 드루젠 및 우안 황반부의 망막하 섬유화 변화와 색소침착이 관찰되었다. 빛간섭단층촬영상 양안 망막색소상피층하 광범위한 고반사 물질의 침착과 우안 소량의 망막내액이 관찰되었다. 이 침착물은 형광안저혈관조영술상 과형광, 인도시아닌그린혈관조영술상 저형광, 안저자가형광검사상 과형광 반점 소견으로 관찰되었다. 분자유전검사 결과 EFEMP1 유전자의 병원성 변이인 이형 접합 c.1033C>T p.Arg345Trp이 검출되어 상염색체 우성 드루젠으로 확진할 수 있었다. 우안 아바스틴 주입술을 시행하였으나, 주사 후 망막내액 및 시력의 호전은 없었다. 결론: 상염색체 우성 드루젠은 매우 드문 질환이나, 일반적인 나이관련황반변성과는 호발 연령 및 드루젠의 임상 양상이 다르고, 항혈관내피성장인자 치료의 반응 또한 차이를 보일 수 있어 감별이 필요하다.

Optimized Production of 2,3-Butanediol by a Lactate Dehydrogenase-deficient Mutant of Klebsiella pneumoniae

Sung-Mok Lee,오백록,Jang Min Park,Anna Yu,Sun-Yeon Heo,Won-Kyung Hong,JEONG-WOO SEO,CHUL HO KIM 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6

To obtain high-yield production of 2,3-butanediol(2,3-BD) from glucose, we optimized the culture conditionsfor a lactate dehydrogenase-deficient mutant (ΔldhA) ofKlebsiella pneumoniae using response surface methodology. 2,3-BD production was successfully improved by optimizingpH (5.6), aeration (3.50 vvm) and concentration of cornsteep liquor (45.0 mL/L) as a nitrogen source, resulting ina maximum level of 2,3-BD production of 148.8 g/L andproductivity of 2.48 g/L/h. 2,3-BD was also obtained withhigh concentration (76.24 g/L) and productivity (2.31 g/L/h)from the K. pneumoniae mutant strain using sugarcanemolasses as a carbon source.

항균활성 특성을 지닌 락토바실러스 루테리 BR301 균주의 분리 및 동정

윤슬기,윤주성,손은심,오백록,윤복근 한국산학기술학회 2024 한국산학기술학회논문지 Vol.25 No.2

In this study, feces from domestic companion animals were used to select strains with excellent acid tolerance, bile tolerance, antioxidant activity, intestinal epithelial cell adhesion, anti-inflammatory effect, and antibacterial activity against harmful microorganisms using the companion animal-derived microbiome. About 110 types of feces-derived lactic acid bacteria were isolated and analyzed for primary and secondary acid resistance and bile resistance, and 10 excellent strains were identified. Then, antioxidant activity, intestinal adhesion ability, and anti-inflammatory properties of these 10 strains were investigated. As a result, homology analysis of the 16S rRNA base sequence was performed on the strain with the best probiotic properties, and the strain was named Lactobacillus reuteri BR301. The antibacterial activity of Lactobacillus reuteri BR301 was not shown against Candida albicans (KCTC 7965), and strong antibacterial activity was observed against Gram-positive and Gram-negative bacteria, including food poisoning strains. Therefore, Lactobacillus reuteri BR301 strain is superior to existing probiotic strains in acid tolerance, bile tolerance, antioxidant activity, intestinal epithelial cell adhesion, anti-inflammatory effect, and antibacterial activity against harmful microorganisms, making it a complex benefit for companion animals. It is believed that it can be used as a beneficial microbial material for companion animals.

Quiescence and Subsequent Anterior Chamber Inflammation in Adalimumab-treated Pediatric Noninfectious Uveitis

Sooyeon Choe,Jang Won Heo,오백록 대한안과학회 2020 Korean Journal of Ophthalmology Vol.34 No.4

Purpose: To evaluate the effect of adalimumab in pediatric uveitis and subsequent changes in anterior chamber inflammationfollowing the inactivation of uveitis. Methods: In this retrospective study, patients with noninfectious uveitis younger than 18 years of age who were treatedwith adalimumab for more than 12 months were included. The rate of complete suppression and the relapse in anteriorchamber inflammation following the initiation of adalimumab therapy were evaluated using anterior chamber cell score andlaser flare photometry (LFP) values, if available. Changes in visual acuity and the sparing effect of topical steroid agents werealso evaluated. Results: Among 22 eyes of 12 pediatric uveitis patients enrolled, 13 eyes were associated with juvenile idiopathic arthritisand nine eyes had idiopathic uveitis. The mean ± standard deviation age was 10.2 ± 3.6 years. Types of uveitis included anterioruveitis (n = 17) and panuveitis (n = 5). Quiescence was observed in 14 eyes (63.6%) at 3 months and in 21 eyes (95.5%) at12 months after initiation, respectively. After achieving inactive uveitis, uveitis relapsed in two eyes at 6 months, even withadalimumab treatment. In 11 eyes, anterior chamber showed 0.5+ cell scores during the rest of the follow-up period and oneof those eyes met the criteria for the relapse based on LFP values. The dosage of topical steroids decreased significantly at3, 9, and 12 months after the initiation of therapy (p ≤ 0.05). Visual acuity did not show improvement. There were no severeadverse effects of anti-tumor necrosis factor-α treatment reported. Conclusions: In this study, adalimumab achieved a quiescent state in most eyes with pediatric noninfectious uveitis for 12months with a relapse rate of 9.5%. LFP values together with the anterior chamber cell score can be utilized to monitor theimprovement or relapse in anterior chamber inflammation in pediatric noninfectious uveitis.

Saccharification of soluble starch and ethanol production by isolated microbial strains

JUNG KON KIM(김중곤),BAEK ROCK OH(오백록),SI WOUK KIM(김시욱) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10