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어현지 ( Hyun Ji Eo ),손용환 ( Yong Hwan Son ),박성혁 ( Sung Hyuk Park ),박광훈 ( Gwang Hun Park ),이경철 ( Kyeong Cheol Lee ),손호준 ( Ho Jun Son ) 한국산림과학회 2021 한국산림과학회지 Vol.110 No.2
This study aims to optimize the appropriate concentration of fertilizers for Sageretia thea by analyzing growth performances (height and root collar diameter) and physiological characteristics (photosynthesis, chlorophyll contents, and chlorophyll fluorescence reaction). As fertilizer concentration was increased to 1.5 g·L<sup>-1</sup>, growth increased, but it decreased at 2.0 g·L<sup>-1</sup> treatment. Root collar diameter growth was reduced because of higher fertilizer concentrations. Photosynthesis reactions showed the highest CO<sub>2</sub> reaction curves, maximum photosynthesis rate, and maximum carboxylation rate in the 1.5 g·L<sup>-1</sup> fertilizer treatment. The chlorophyll fluorescence reaction and SPAD values revealed that fertilizer treatment improves photosynthesis efficiency and robustness compared with untreated control. Therefore, the appropriate fertilizer concentration for producing good seedling quality of Sageretia thea is 1.0~1.5 g·L<sup>-1</sup>.
작약의 Albiflorin, Paeoniflorin 함량과 토양특성 간의 상관관계 연구
어현지(Hyun Ji Eo),박영기(Youngki Park),박광훈(Gwang Hun Park),김지아(Ji-Ah Kim),김다솜(Da Som Kim),강연경(Yeongyeong Kang),김기윤(Kiyoon Kim),장준혁(Jun Hyuk Jang),김현준(Hyun-Jun Kim) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.4
The aim of this study was to investigate the correlation between soil properties and marker compounds contents of Paeonia lactiflora. The methods of determining marker compounds were validated by measuring the linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy and recovery using UPLC analysis. P. lactiflora contained albiflorin at 0.04 ± 0.00 ~ 2.79 ± 0.21%, paeoniflorin at 1.98 ± 0.14 ~ 6.67 ± 0.84%. The root dry weight (RDW) of P. lactiflora was 0.06 ± 0.02 ~ 1.27 ± 0.28 ㎏. The soil properties analysis such as soil pH, electric conductivity (EC), organic matter (OM), total nitrogen (TN), available phosphate (Avail. P2O5), exchangeable cation and cation exchange capacity (CEC) were performed following standard analysis manual. The results of correlation analysis between soil properties and growth characteristics, available P2O5 was positively coreelated with the RDW of P. lactiflora. On the other hand, the RDW of P. lactiflora showed significantly negative correlation with contents of albiflorin and paeoniflorin. The results of this study was might be help to provide useful information on the establish of standard cultivation by the investigate correlation analysis between growth characteristics and marker compound contents of P. lactiflora. 본 연구는 작약의 재배지별 토양특성과 albiflorin, paeoniflorin 함량 간의 상관관계를 구명하기 위하여 수행되었다. UPLC를 사용하여 직선성, LOD, LOQ, 정밀성, 정확성, 회수율을 평가하여 분석법을 검증하였다. 작약의 albiflorin 함량은0.04 ± 0.00 ~ 2.79 ± 0.21%, paeoniflorin은 1.98 ± 0.14 ~ 6.67 ± 0.84%로 확인되었다. 또한 작약의 개체별 뿌리 중량을조사한 결과, 0.06 ± 0.02 ~ 1.27 ± 0.28 ㎏로 지역별 차이를확인하였다. 작약 재배지의 토양특성은 pH, 전기전도도, 유기물 함량, 질소전량, 유효인산, 치환성 칼륨, 치환성 칼슘, 치환성 마그네슘, 치환성 나트륨, 양이온치환용량 등을 분석하였다. 재배지 토양특성과 작약 뿌리 중량 간의 상관관계를 분석한 결과, 토양의 유효인산과 작약 뿌리 중량 간의 유의적인 정의 상관관계를 확인하였다. 또한 작약 뿌리 중량과 지표성분 함량 간의상관관계를 분석한 결과, 작약 뿌리 중량은 지표성분인 albiflorin, paeoniflorin의 함량과 유의적인 부의 상관관계를 보이는 것을확인하였다. 따라서 작약 재배에 있어 지표성분과 생산량을 동시에 증가시키는 재배법 개발에 유용한 정보를 제공할 것으로판단된다.
망개나무 추출물의 NF-κB 및 MAPK 신호전달 억제를 통한 항염증 효과
김다솜(Da Som Kim),어현지(Hyun Ji Eo),강연경(Yeongyeong Kang),박광훈(Gwang Hun Park) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.1
Berchemia berchemiaefolia (Makino) Koidz. is found not only in korea, but also in japan and is known as a rare plant all over the world. In this study, we evaluated anti-inflammatory effect of B. berchemiaefolia (Makino) Koidz. leaves (BBK-L) in LPS-stimulated RAW264.7 cells. BBK-L inhibited the generation of NO through the suppression of iNOS experession. BBK-L attenuated the expression of iNOS, COX-2, TNF-α, IL-1β, IL-6 induced by LPS. BBK-L decreased LPS-mediated IκB-α degradation inhibite which resulted in the inhibition of NF-κB activation in RAW264.7 cells. In addition, BBK-L suppressed ERK1/2, JNK phosphorylation induced by LPS. BBK-L showed anti-inflammatory effect through blocking the generation of the inflammatory mediators such as NO, iNOS, COX-2, TNF-α, IL-1β, IL-6 via the inhibiting of NF-κB and MAPK signaling activation. These results suggests that BBK-L may have great potential for the development of anti-inflammatory drug to treat acute and chronic inflammatory disorders.
적변에 따른 연근별 산양삼 생육특성과 진세노사이드 함량
김기윤(Kiyoon Kim),어현지(Hyun-Ji Eo),김현준(Hyun-Jun Kim),엄유리(Yurry Um),정대희(Dae-Hui Jeong),허정훈(Jeong-Hoon Huh),전권석(Kwon-Seok Jeon) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.5
The aim of this study was to investigate the growth characteristic and ginsenoside contents of 7 and 13 year-old wild-simulated ginseng (Panax ginseng C.A. Meyer) according to rusty root. The root growth characteristics of wild-simulated ginseng were did not shows significant difference according to the rusty root. The results of ginsenoside contents of wild-simulated ginseng according to rusty root, ginsenoside Rb1 and Rg1 of 7 year-old wild-simulated ginseng were had shows a significantly higher in rusty root compare to general root. On the other hand, ginsenodie Rc, Rd, Re and Rg2 were significantly higher in gerneral root. In the case of 13 year-old wild-simulated ginseng, the contents of ginsenoside did not shows to significant difference according to rusty root. The results of correlation analysis between growth characteristics and ginsenoside content of general root, the ginsenoside Rb2, Rc, Rd, Rf, Rg1 were positive correlation with root length, while as the ginsenoside Rd of rusty root was shows significantly negative correlation with root length. The results of this study was might be able to improve awareness of consumer related to rusty root of wild-simulated ginseng. Moreover, might be help to provide useful information on the establish quality standard and distribution system of wild-simulated ginseng.
짝자래나무[Rhamnus yoshinoi] 가지 추출물에 의한 전립선암세포의 Wnt/β-catenin 분해 유도 활성 및 GC/MS 분석
강연경(Yeongyeong Kang),어현지(Hyun Ji Eo),김다솜(Da Som Kim),박영기(Youngki Park),박광훈(Gwang Hun Park) 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.2
We evaluated the anti-cancer activity against human prostate cancer cells and the associated molecular mechanism of extracts from the branches of Rhamnus yoshinoi (RYB). Treatment with RYB suppressed viability of human prostate cancer cells (PC-3) and decreased protein levels of both β-catenin and T-cell factor 4 (TCF4). This was reflected in reduced TCF4 mRNA, but not decreased β- catenin mRNA. PC-3 cells were pretreated with the proteosome inhibitor MG132 before treatment with RYB, which blocked RYB-mediated down regulation of β-catenin in PC-3 cells, thus confirming that RYB promotes the proteasomal degradation of β-catenin. RYB induced β-catenin phosphorylation, and GSK-3β inhibition by LiCl blocked the phosphorylation and proteasomal degradation of β- catenin by RYB. These results suggest that GSK-3β may be an important upstream kinase for RYB-mediated regulation of β-catenin. Finally, GC/MS analysis of RYB identified 18 compounds. Based on these findings, RYB shows potential for development as a therapeutic agent for prostate cancer
상동나무(Sageretia thea) 가지추출물의 대장암세포에서 NF-κB 신호전달 활성화를 통한 세포사멸 유도활성
김정동(Jeong Dong Kim),박수빈(Su Bin Park),어현지(Hyun Ji Eo),박광훈(Gwang Hun Park),정진부(Jin Boo Jeong) 한국자원식물학회 2020 한국자원식물학회지 Vol.33 No.5
본 연구에서 상동나무 가지 추출물(STB-E100)은 대장암 세포에서 세포사멸을 유도하여 세포생육을 억제하였다. 또한 Iκ B-α인산화를 통한 IκB-α단백질 분해를 유도하며 이로 인해P65 핵내 전이를 유도하여 NF-κB 신호전달을 활성화시킨다. NF-κB 신호전달 활성화는 GSK3β활성화를 통해 P65 핵내 전이를 유도에 의한 것이지만 IκB-α분해는 GSK3β의존성이 아니다. 상동나무 가지 추출물은 이러한 신호전달 활성화를 통해 세포사멸을 유도하여 대장암의 세포생육을 억제한다. 본 결과를바탕으로 상동나무 가지가 암 예방 및 치료를 목적으로 한 표적요법에서 항암제 개발의 잠재적 활용 소재로서 이용 가능하다고 사료된다. 그러나 대장암 세포에서 상동나무 가지 추출물에의해 유도된 NF-κB 신호전달 작용기전을 좀더 구체적으로 구명할 필요가 있고 대장암에 대한 세포사멸과 작용기전의 정확한 관련성을 조사하기 위해 추가적인 연구가 필요하다. In this study, we evaluated the inhibitory effect against cell growth and potential molecular mechanism of 100% ethanol extracts of branch from Sageretia thea in human colorectal cancer cells, HCT116. Ethanol dose-dependently extracts of STB significantly suppressed the growth of HCT116 cells through apoptosis. STB activated NF-κB signaling pathway through IκB-α proteasomal degradation and inducing p65 accumulation in nucleus. The inhibition of GSK3β by LiCl didn’t affect STB mediated degradation IκB-α but STB mediated p65 accumulation in nucleus. In addition, STB phosphorylated GSK3β. Based on these findings, STB may be a potential candidate for the development of anti-cancer agents for human colorectal cancer.
일본잎갈나무림과 침활혼효림의 입지환경이 산양삼 종묘의 초기 생육에 미치는 영향
김기윤 ( Kiyoon Kim ),엄유리 ( Yurry Um ),정대희 ( Dae-hui Jeong ),어현지 ( Hyun-ji Eo ),전권석 ( Kwon-seok Jeon ),김현준 ( Hyun-jun Kim ) 한국산림과학회 2020 한국산림과학회지 Vol.109 No.3
This study investigated the environmental location effects that Larix kaempferi and mixed forests had on the early growth of wild-simulated ginseng (Panax ginseng C. A. Meyer) seedlings. Experimental plots were created in Larix kaempferi and mixed forests, and confirmed the location environments and growth characteristics of wild-simulated ginseng seedling. Our results showed that the soil properties, such as soil organic matter, total nitrogen, and cation exchangeable capacity were significantly higher in the mixed forest soil compared to the Larix kaempferiforest soil. Likewise, the growth characteristics of wild-simulated ginseng seedlings were significantly higher in mixed forestsd compare to the Larix kaempferi forest. Correlation analysis between tree species ratio, soil properties, and growth characteristics in the experimental plots determined that the soil properties and growth characteristics had significant positive correlation with the broad-leaved forest percentage. Growth characteristics of the wild-simulated ginseng seedlings were shown to have a significant positive correlation with organic matter, total nitrogen, and cation exchange capacities. This study has clearly demonstrated that the tree species ratio, and soil properties in Larix kaempferi and mixed forests were significantly correlated with the early growth of wildsimulated ginseng seedlings. These results could help to improve the selection of suitable cultivation sites for wild-simulated ginseng.
이산화염소의 활성산소 생성 유도에 의한 항암 및 항바이러스 활성
김용균 ( Yong Gyun Kim ),수닐쿠마르 ( Sunil Kumar ),천원수 ( Won Su Cheon ),어현지 ( Hyun Ji Eo ),권혁 ( Hyeok Kwon ),전용호 ( Yong Ho Jeon ),정진부 ( Jin Boo Jung ),김욱 ( Wook Kim ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.1
Chlorine dioxide has been used for a disinfectant by exhibiting antimicrobial activity and is also potent to kill insect pests infesting stored grains. This study aimed to extend the usefulness of chlorine dioxide with respect to anticancer and antiviral activities. Cytotoxicity of chlorine dioxide was assessed against five different human cancer cell lines. Chlorine dioxide exhibited significant cytotoxicity against two breast cancer cell lines (MCF-7, MDA-MB-231) and three colorectal cancer cell lines (LoVo, HCT-116, SW-480). This cytotoxicity appeared to be ass℃iated with the capacity of chlorine dioxide to induce the production of reactive oxygen species (ROS). Compared to control insect cell lines, the cancer cell lines possessed much higher levels of ROS. On the other hand, a treatment of an antioxidant, vitamin E, significantly reduced the cytotoxicity, suggesting that the cytotoxicity was induced by high levels of ROS production. Chlorine dioxide exhibited antiviral activity against different viruses. A baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), is a dsDNA insect virus and lost its viral activity to form polyhedral viral particles in response to chlorine dioxide. The antiviral activity against AcNPV was dependent on the incubation time with chlorine dioxide. Tobacco mosaic virus is a ssRNA plant virus and was reduced in its population after exposure to chlorine dioxide along with significant decrease of viral symptoms. These results indicate that chlorine dioxide possesses anticancer and antiviral activities probably due to its inducing activity of ROS production.